1LM5 : Structures of two intermediate filament-binding fragments of desmoplakin reveal a unique repeat motif structure

  • Hee Jung Choi (Contributor)
  • Shaun Park-Snyder (Contributor)
  • Lauren T. Pascoe (Contributor)
  • Kathleen Janee Green (Contributor)
  • William I. Weis (Contributor)



Experimental Technique/Method:X-RAY DIFFRACTION
Release Date:2002-07-31
Deposition Date:2002-04-30
Revision Date:2008-04-28#2011-07-13
Molecular Weight:46599.05
Macromolecule Type:Protein
Residue Count:428
Atom Site Count:2928

Desmosomes are intercellular junctions in which cadherin cell adhesion molecules are linked to the intermediate filament (IF) system. Desmoplakin is a member of the plakin family of IF-binding proteins. The C-terminal domain of desmoplakin (DPCT) mediates binding to IFs in desmosomes. The DPCT sequence contains three regions, termed A, B and C, consisting of 4.5 copies of a 38-amino acid repeat motif. We demonstrate that these regions form discrete subdomains that bind to IFs and report the crystal structures of domains B and C. In contrast to the elongated structures formed by other kinds of repeat motifs, the plakin repeats form a globular structure with a unique fold. A conserved basic groove found on the domain may represent an IF-binding site.
Date made available2002

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