1NZG : Crystal structure of A-DNA decamer GCGTA(3ME)ACGC, with a modified 5-methyluridine

  • Marija Prhavc (Contributor)
  • Thazha P. Prakash (Contributor)
  • George Minasov (Contributor)
  • Martin Egli (Contributor)
  • Muthiah Manoharan (Contributor)

Dataset

Description

Experimental Technique/Method:X-RAY DIFFRACTION
Resolution:1.6
Classification:DNA
Release Date:2003-08-26
Deposition Date:2003-02-17
Revision Date:2008-04-29#2011-07-13#2011-11-02
Molecular Weight:6352.36
Macromolecule Type:DNA
Residue Count:20
Atom Site Count:422
DOI:10.2210/pdb1nzg/pdb

Abstract:
[structure: see text] Oligonucleotides with a novel, 2'-O-[2-[2-(N,N-dimethylamino)ethoxy]ethyl] (2'-O-DMAEOE) modification have been synthesized. This modification, a cationic analogue of the 2'-O-(2-methoxyethyl) (2'-O-MOE) modification, exhibits high binding affinity to target RNA (but not to DNA) and exceptional resistance to nuclease degradation. Analysis of the crystal structure of a self-complementary oligonucleotide containing a single 2'-O-DMAEOE modification explains the importance of charge factors and gauche effects on the observed antisense properties. 2'-O-DMAEOE modified oligonucleotides are ideal candidates for antisense drugs.
Date made available2003
PublisherRCSB-PDB

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