Description
Experimental Technique/Method:X-RAY DIFFRACTION
Resolution:2.5
Classification:TRANSCRIPTION
Release Date:2003-09-16
Deposition Date:2003-07-15
Revision Date:2008-04-29#2011-07-13
Molecular Weight:30906.27
Macromolecule Type:Protein
Residue Count:270
Atom Site Count:1974
DOI:10.2210/pdb1q07/pdb
Abstract:
The earliest of a series of copper efflux genes in Escherichia coli are controlled by CueR, a member of the MerR family of transcriptional activators. Thermodynamic calibration of CueR reveals a zeptomolar (10(-21) molar) sensitivity to free Cu+, which is far less than one atom per cell. Atomic details of this extraordinary sensitivity and selectivity for +1transition-metal ions are revealed by comparing the crystal structures of CueR and a Zn2+-sensing homolog, ZntR. An unusual buried metal-receptor site in CueR restricts the metal to a linear, two-coordinate geometry and uses helix-dipole and hydrogen-bonding interactions to enhance metal binding. This binding mode is rare among metalloproteins but well suited for an ultrasensitive genetic switch.
Resolution:2.5
Classification:TRANSCRIPTION
Release Date:2003-09-16
Deposition Date:2003-07-15
Revision Date:2008-04-29#2011-07-13
Molecular Weight:30906.27
Macromolecule Type:Protein
Residue Count:270
Atom Site Count:1974
DOI:10.2210/pdb1q07/pdb
Abstract:
The earliest of a series of copper efflux genes in Escherichia coli are controlled by CueR, a member of the MerR family of transcriptional activators. Thermodynamic calibration of CueR reveals a zeptomolar (10(-21) molar) sensitivity to free Cu+, which is far less than one atom per cell. Atomic details of this extraordinary sensitivity and selectivity for +1transition-metal ions are revealed by comparing the crystal structures of CueR and a Zn2+-sensing homolog, ZntR. An unusual buried metal-receptor site in CueR restricts the metal to a linear, two-coordinate geometry and uses helix-dipole and hydrogen-bonding interactions to enhance metal binding. This binding mode is rare among metalloproteins but well suited for an ultrasensitive genetic switch.
| Date made available | 2003 |
|---|---|
| Publisher | RCSB-PDB |