1ZTM : Structure of the Uncleaved Paramyxovirus (hPIV3) Fusion Protein

  • Reay G. Paterson (Contributor)
  • Robert A Lamb (Contributor)
  • Theodore S. Jardetzky (Contributor)
  • Hsien Shen Yin (Contributor)
  • Xiaolin Wen (Contributor)



Experimental Technique/Method:X-RAY DIFFRACTION
Classification:VIRAL PROTEIN
Release Date:2005-07-19
Deposition Date:2005-05-27
Revision Date:2008-04-30#2011-07-13#2017-10-11
Molecular Weight:163175.92
Macromolecule Type:Protein
Residue Count:1470
Atom Site Count:9431

Class I viral fusion proteins share common mechanistic and structural features but little sequence similarity. Structural insights into the protein conformational changes associated with membrane fusion are based largely on studies of the influenza virus hemagglutinin in pre- and postfusion conformations. Here, we present the crystal structure of the secreted, uncleaved ectodomain of the paramyxovirus, human parainfluenza virus 3 fusion (F) protein, a member of the class I viral fusion protein group. The secreted human parainfluenza virus 3 F forms a trimer with distinct head, neck, and stalk regions. Unexpectedly, the structure reveals a six-helix bundle associated with the postfusion form of F, suggesting that the anchor-minus ectodomain adopts a conformation largely similar to the postfusion state. The transmembrane anchor domains of F may therefore profoundly influence the folding energetics that establish and maintain a metastable, prefusion state.
Date made available2005

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