2W0F : Potassium Channel KcsA-Fab Complex with Tetraoctylammonium

  • Michael J. Lenaeus (Contributor)
  • Tobias Wagner (Contributor)
  • Pamela J Focia (Contributor)
  • Adrian Gross (Contributor)

Dataset

Description

Experimental Technique/Method:X-RAY DIFFRACTION
Resolution:2.4
Classification:IMMUNE SYSTEM/METAL TRANSPORT
Release Date:2009-11-17
Deposition Date:2008-08-14
Revision Date:2014-08-27#2014-09-03
Molecular Weight:61653.38
Macromolecule Type:Protein
Residue Count:555
Atom Site Count:4239
DOI:10.2210/pdb2w0f/pdb

Abstract:
Potassium channels allow for the passive movement of potassium ions across the cell membrane and are instrumental in controlling the membrane potential in all cell types. Quaternary ammonium (QA) compounds block potassium channels and have long been used to study the functional and structural properties of these channels. Here we describe the interaction between three symmetrical hydrophobic QAs and the prokaryotic potassium channel KcsA. The structures demonstrate the presence of a hydrophobic pocket between the inner helices of KcsA and provide insight into the binding site and blocking mechanism of hydrophobic QAs. The structures also reveal a structurally hidden pathway between the central cavity and the outside membrane environment reminiscent of the lateral fenestration observed in sodium channels that can be accessed through small conformational changes in the pore wall. We propose that the hydrophobic binding pocket stabilizes the alkyl chains of long-chain QA molecules and may play a key role in hydrophobic drug binding in general.
Date made available2009
PublisherRCSB-PDB

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