Description
Experimental Technique/Method:X-RAY DIFFRACTION
Resolution:2.45
Classification:TRANSCRIPTION
Release Date:2011-06-08
Deposition Date:2011-05-17
Revision Date:2011-07-13#2012-01-11
Molecular Weight:22104.85
Macromolecule Type:Protein
Residue Count:191
Atom Site Count:1299
DOI:10.2210/pdb3s32/pdb
Abstract:
Absent, small or homeotic discs-like 2 (ASH2L) is a trithorax group (TrxG) protein and a regulatory subunit of the SET1 family of lysine methyltransferases. Here we report that ASH2L binds DNA using a forkhead-like helix-wing-helix (HWH) domain. In vivo, the ASH2L HWH domain is required for binding to the β-globin locus control region, histone H3 Lys4 (H3K4) trimethylation and maximal expression of the β-globin gene (Hbb-1), validating the functional importance of the ASH2L DNA binding domain.
Resolution:2.45
Classification:TRANSCRIPTION
Release Date:2011-06-08
Deposition Date:2011-05-17
Revision Date:2011-07-13#2012-01-11
Molecular Weight:22104.85
Macromolecule Type:Protein
Residue Count:191
Atom Site Count:1299
DOI:10.2210/pdb3s32/pdb
Abstract:
Absent, small or homeotic discs-like 2 (ASH2L) is a trithorax group (TrxG) protein and a regulatory subunit of the SET1 family of lysine methyltransferases. Here we report that ASH2L binds DNA using a forkhead-like helix-wing-helix (HWH) domain. In vivo, the ASH2L HWH domain is required for binding to the β-globin locus control region, histone H3 Lys4 (H3K4) trimethylation and maximal expression of the β-globin gene (Hbb-1), validating the functional importance of the ASH2L DNA binding domain.
| Date made available | 2011 |
|---|---|
| Publisher | RCSB-PDB |