Description
Experimental Technique/Method:X-RAY DIFFRACTION
Resolution:1.9
Classification:STRUCTURAL GENOMICS, UNKNOWN FUNCTION
Release Date:2013-05-29
Deposition Date:2013-05-08
Revision Date:2016-11-23#2017-11-15
Molecular Weight:122714.66
Macromolecule Type:Protein
Residue Count:1084
Atom Site Count:8424
DOI:10.2210/pdb4kmq/pdb
Abstract:
Here we employ a 'systems structural biology' approach to functionally characterize an unconventional α-glucan metabolic pathway from the food-borne pathogen Listeria monocytogenes (Lm). Crystal structure determination coupled with basic biochemical and biophysical assays allowed for the identification of anabolic, transport, catabolic and regulatory portions of the cycloalternan pathway. These findings provide numerous insights into cycloalternan pathway function and reveal the mechanism of repressor, open reading frame, kinase (ROK) transcription regulators. Moreover, by developing a structural overview we were able to anticipate the cycloalternan pathway's role in the metabolism of partially hydrolysed starch derivatives and demonstrate its involvement in Lm pathogenesis. These findings suggest that the cycloalternan pathway plays a role in interspecies resource competition-potentially within the host gastrointestinal tract-and establish the methodological framework for characterizing bacterial systems of unknown function.
Resolution:1.9
Classification:STRUCTURAL GENOMICS, UNKNOWN FUNCTION
Release Date:2013-05-29
Deposition Date:2013-05-08
Revision Date:2016-11-23#2017-11-15
Molecular Weight:122714.66
Macromolecule Type:Protein
Residue Count:1084
Atom Site Count:8424
DOI:10.2210/pdb4kmq/pdb
Abstract:
Here we employ a 'systems structural biology' approach to functionally characterize an unconventional α-glucan metabolic pathway from the food-borne pathogen Listeria monocytogenes (Lm). Crystal structure determination coupled with basic biochemical and biophysical assays allowed for the identification of anabolic, transport, catabolic and regulatory portions of the cycloalternan pathway. These findings provide numerous insights into cycloalternan pathway function and reveal the mechanism of repressor, open reading frame, kinase (ROK) transcription regulators. Moreover, by developing a structural overview we were able to anticipate the cycloalternan pathway's role in the metabolism of partially hydrolysed starch derivatives and demonstrate its involvement in Lm pathogenesis. These findings suggest that the cycloalternan pathway plays a role in interspecies resource competition-potentially within the host gastrointestinal tract-and establish the methodological framework for characterizing bacterial systems of unknown function.
Date made available | 2013 |
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Publisher | RCSB-PDB |