4Y5F : PAS-GAF fragment from Deinococcus radiodurans BphP assembled with BV - Y307S, high dose

  • Feifei Li (Contributor)
  • E. Sethe Burgie (Contributor)
  • Tao Yu (Contributor)
  • Annie Héroux (Contributor)
  • George C Schatz (Contributor)
  • Richard D. Vierstra (Contributor)
  • Allen M. Orville (Contributor)

Dataset

Description

Experimental Technique/Method:X-RAY DIFFRACTION
Resolution:1.7
Classification:TRANSFERASE
Release Date:2015-05-20
Deposition Date:2015-02-11
Revision Date:2017-09-06#2017-11-22
Molecular Weight:35048.99
Macromolecule Type:Protein
Residue Count:319
Atom Site Count:2570
DOI:10.2210/pdb4y5f/pdb

Abstract:
We report that in the red light-absorbing (Pr) state, the bilin chromophore of the Deinococcus radiodurans proteobacterial phytochrome (DrBphP) is hypersensitive to X-ray photons used in typical synchrotron X-ray protein crystallography experiments. This causes the otherwise fully protonated chromophore to deprotonate without additional major structural changes. These results have major implications for our understanding of the structural and chemical characteristics of the resting and intermediate states of phytochromes and other photoreceptor proteins.
Date made available2015
PublisherRCSB-PDB

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