Description
Experimental Technique/Method:X-RAY DIFFRACTION
Resolution:2.2
Classification:VIRUS
Release Date:2016-09-07
Deposition Date:2015-05-06
Revision Date:2016-09-28
Molecular Weight:69122.39
Macromolecule Type:Protein
Residue Count:645
Atom Site Count:4772
DOI:10.2210/pdb4zor/pdb
Abstract:
Virus-like particles are used to encapsulate drugs, imaging agents, enzymes, and other biologically active molecules in order to enhance their function. However, the size of most virus-like particles is inflexible, precluding the design of appropriately sized containers for different applications. Here, we describe a chromatographic selection for virus-like particle assembly. Using this selection, we identified a single amino acid substitution to the coat protein of bacteriophage MS2 that mediates a uniform switch in particle geometry from T = 3 to T = 1 icosahedral symmetry. The resulting smaller particle retains the ability to be disassembled and reassembled in vitro and to be chemically modified to load cargo into its interior cavity. The pair of 27 and 17 nm MS2 particles will allow direct examination of the effect of size on function in established applications of virus-like particles, including drug delivery and imaging.
Resolution:2.2
Classification:VIRUS
Release Date:2016-09-07
Deposition Date:2015-05-06
Revision Date:2016-09-28
Molecular Weight:69122.39
Macromolecule Type:Protein
Residue Count:645
Atom Site Count:4772
DOI:10.2210/pdb4zor/pdb
Abstract:
Virus-like particles are used to encapsulate drugs, imaging agents, enzymes, and other biologically active molecules in order to enhance their function. However, the size of most virus-like particles is inflexible, precluding the design of appropriately sized containers for different applications. Here, we describe a chromatographic selection for virus-like particle assembly. Using this selection, we identified a single amino acid substitution to the coat protein of bacteriophage MS2 that mediates a uniform switch in particle geometry from T = 3 to T = 1 icosahedral symmetry. The resulting smaller particle retains the ability to be disassembled and reassembled in vitro and to be chemically modified to load cargo into its interior cavity. The pair of 27 and 17 nm MS2 particles will allow direct examination of the effect of size on function in established applications of virus-like particles, including drug delivery and imaging.
| Date made available | 2016 |
|---|---|
| Publisher | RCSB-PDB |