Description
Accession Number: GSE12493
Platform:
GPL6480: Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
GPL6848: Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Probe Name version)
Organism: Homo sapiens
Published on 2009-09-15
Summary:
Primary dermal fibroblasts from patients with dSSc and healthy controls were treated with TGF beta for up to 24h and the genome-wide patterns of gene expression measured on DNA microarrays. 894 genes were identified as TGF beta-responsive in 4 independent cultures of dermal fibroblasts (2 healthy control and 2 dSSc patients). The 894 genes in the TGF beta-responsive signature are associated with induction of growth factor signaling, collagen synthesis and extracellular matrix deposition.
Overall Design:
Common reference design; we used Universal Human Reference RNA (Stratagene) as our reference for every array; For every hybridization sample RNA was always labeled with Cy3, and reference RNA was always labeled with Cy5 All processed data is displayed as log2 of the LOWESS-normalized Cy5/Cy3 ratio and was multiplied by negative 1 for analysis in the associated publication.
Contact:
Name: Jennifer L Sargent
Organization: Dartmouth Medical School
Laboratory: Whitfield
Deparment: Genetics
Address: Dartmouth College; HB7400 Hanover NH 03755 USA
Email: [email protected]
Organization: Agilent Technologies
Address: Palo Alto CA 94304 USA
Email: [email protected]
Phone: 877-424-4536
Web-Link: www.agilent.com
Platform:
GPL6480: Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
GPL6848: Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Probe Name version)
Organism: Homo sapiens
Published on 2009-09-15
Summary:
Primary dermal fibroblasts from patients with dSSc and healthy controls were treated with TGF beta for up to 24h and the genome-wide patterns of gene expression measured on DNA microarrays. 894 genes were identified as TGF beta-responsive in 4 independent cultures of dermal fibroblasts (2 healthy control and 2 dSSc patients). The 894 genes in the TGF beta-responsive signature are associated with induction of growth factor signaling, collagen synthesis and extracellular matrix deposition.
Overall Design:
Common reference design; we used Universal Human Reference RNA (Stratagene) as our reference for every array; For every hybridization sample RNA was always labeled with Cy3, and reference RNA was always labeled with Cy5 All processed data is displayed as log2 of the LOWESS-normalized Cy5/Cy3 ratio and was multiplied by negative 1 for analysis in the associated publication.
Contact:
Name: Jennifer L Sargent
Organization: Dartmouth Medical School
Laboratory: Whitfield
Deparment: Genetics
Address: Dartmouth College; HB7400 Hanover NH 03755 USA
Email: [email protected]
Organization: Agilent Technologies
Address: Palo Alto CA 94304 USA
Email: [email protected]
Phone: 877-424-4536
Web-Link: www.agilent.com
| Date made available | Aug 19 2008 |
|---|---|
| Publisher | Gene Expression Omnibus |