Additional file 1: Figure S1. of Expression and functional analysis of the Wnt/beta-catenin induced mir-135a-2 locus in embryonic forebrain development

Rmst expression during forebrain development. (A-F) Rmst ish on coronal sections of wild type brains from E11.5 to adult stage. (A) At E11.5, Rmst is expressed in the epithalamus, medial wall of the dorsal telencephalon, the eminentia thalami, and the septum. (B) At E12.5 (as in Fig. 1), Rmst was detected in the medial wall of the telencephalon and in the diandephalon. (C) At E14.5, Rmst is still highly expressed in the cortical hem and hippocampus primordium, thalamus and hypothalamus. (D) At E16.5, Rmst expression is high in the hippocampus and fimbria but low in scattered cells of the migratory stream. Expression in the thalamus is maintained. (E) At post-natal stages P1, Rmst is restricted to the fimbria, yet expressed outside of the hippocampal formation to become virtually undetectable in the hippocampus of adult mice (F) (hippocampal staining is not considered specific to the Rmst probe). dm, dorso-medial wall of telencephalon; emT, eminentia thalami; Et, epithalamus; tel, telencephalon; v3, third ventricle; lv, lateral ventricle; Sp, septum; d, diencephalon; hp, hippocampus; ch, cortical hem; cp, choroid plexus; ncx, neocortex; LGE, lateral ganglionic eminences; th, thalamus, hy, hypothalamus; fm, fimbria; ms, migratory stream; CA1 and CA3, hippocampal fieds; DG, dentate gyrus. Scale bar 400 μm in panels A, C and D; 100 μm in panel B; 200 μm in panels E and F. (TIF 1931 kb)