Additional file 10: of BMP-dependent, injury-induced stem cell niche as a mechanism of heterotopic ossification

Figure S7. Gli1-creERT-mediated DTA expression inhibited injury-induced HO. A&B) Typical x-ray images of control (A) and TAM treated (B) Nse-BMP4;Gli-creERT;ROSA26-eGFP-DTA mice after injury. C) HO incidence in control and TAM treated group. D) Quantification of wet weight of HO in the control and TAM treated groups. Note that depletion of Gli1-creERT-labeled cells partially inhibited but did not completely block HO. E) Typical fluorescence images from TAM treated (E) and control (F) Nse-BMP4;Gli1-creERT;ROSA26-eGFP-DTA mice. Note that in the TAM treated group (E), GFP- (recombined) cells were rarely found. G&H) H&E staining of sections from TAM treated (G) and control (H) Nse-BMP4;Gli1-creERT;ROSA26-eGFP-DTA mice. Note that both fluorescence images and H&E staining suggest that the proposed MSC domain (within dashed lines) was thinner in the TAM treated group. E-H are on the same scale, Bar = 50 μm. (TIF 15685 kb)