Additional file 11: of BMP-dependent, injury-induced stem cell niche as a mechanism of heterotopic ossification

Figure S8. Evidence of depletion of Gli1 in the target cells. The depletion of Gli1 in the target cells was confirmed by staining the tissue sections of Nse-BMP4;Gli1-creERT−/−;Zsgreen mice (A, low power &B, high power), and the tissues of Nse-BMP4;Gli1-creERT+/−;Zsgreen (C, low power &D, high power) mice with Gli1 antibody. Note that there is no specific staining of Gli1 (red) in the lesional tissues from Nse-BMP4;Gli1-creERT−/−;Zsgreen mice, while the specific staining of Gli1 (red) was observed in the Zsgreen+ cells in the proposed MSC niche in lesional tissues from Nse-BMP4;Gli1-creERT+/−;Zsgreen mice. (JPG 822 kb)