Additional file 2: Table S1. of New ligation independent cloning vectors for expression of recombinant proteins with a self-cleaving CPD/6xHis-tag

List of proteins examined for expression and solubility. IDP# Identification protein number relative to CSGID targets (http://csgid.org/); Locus ID for NCBI; Expression: expressed proteins are highlighted in yellow (1-Low expression, 2-Medium expression, 3-High expression). Non-expressed proteins are highlighted in white; Solubility: soluble proteins are highlighted in yellow (1-Low solubility, 2-Medium solubility, 3-High solubility). Non-soluble proteins are highlighted in white (0-undetectable solubility); Purified: proteins purified by single step on Ni-NTA resin and desalting. Purified proteins are highlighted in yellow (1-Low solubility, 2-Medium, 3-High). Non-purified proteins are highlighted in white (0-undetectable); InsP6 cleavage: proteins cleaved by InsP6 CPD autoprocessing activation and purified by â negativeâ Ni-NTA purification step. Soluble proteins after the cleavage are highlighted in yellow (1-Low solubility, 2-Medium, 3-High). Proteins lost after InsP6-cleavage/Ni-NTA purification are highlighted in white (0-undetectable). (XLSX 16 kb)