Additional file 5: of BMP-dependent, injury-induced stem cell niche as a mechanism of heterotopic ossification

Figure S3. Validation of the dual-pulse CldU and IdU labeling procedure. To validate this procedure, we used two positive controls (A&B), and two negative controls (C&D). As expected, we observed plentiful specific staining, including CldU+/IdU− (red arrows), CldU+/IdU+ (yellow arrows) and CldU−/IdU+ (green arrows) cells in intestinal mucosa (A) & hair follicles (B) but not in muscle (C&D). E) Experimental paradigm for dual-pulse labeling procedure. A-D are on the same scale, Bar = 50 μm. (TIF 9688 kb)