Additional file 8: Figure S8. of Expression and functional analysis of the Wnt/beta-catenin induced mir-135a-2 locus in embryonic forebrain development

Expression of bioinformatically predicted miR-135a targets. (A-D) Coronal sections of E12.5 controls (mir-135a-2OE) and mutant (Emx1::Cre;mir-135a2-OE) brains, processed for immunohistochemistry (IHC) (A-B) or in situ hybridization (C, D). phospho-Smad (1/5/8) IHC on control (A) and mutant (B) brain coronal sections shows reduced signal in the cortical hem and the choroid plexus domains of mutants (arrowheads). Panels C and D show a reduced Msx2 expression domain. ch, cortical hem; cp, choroid plexus. Scale bar 25 μm in panels A and B; 50 μm in panels C and D. (TIF 5061 kb)