Additional file 9 of RNA-based amplicon sequencing is ineffective in measuring metabolic activity in environmental microbial communities

Additional file 9: Supplementary Figure 3. Determining microbial biomass of realistic community samples with(out) spike-in (part 2). qPCR was performed targeting 16S rRNA gene V4 region in DNA and RNA (cDNA) extractions from samples collected from four computer screens, four computer mice, three human saliva and three soil. Each subject was sampled in parallel, with one set spiked with 102CFU (computer screens and computer mice) or 107 CFU (saliva and soil) E. coli culture.