CDC25A is a critical target of checkpoint, and its overexpression is observed in various cancers. Here we demonstrate that in vivo levels of Cdc25A expression determine the efficiency of transformation and tumorigenesis. Transgenic expression of CDC25A in murine mammary glands cooperates with tumorigenesis induced by expression of ras or neu. CDC25A- overexpressing tumors display aggressiveness and genomic instability with changes in fragile chromosomal regions, including the region orthologous to human 1p32-36. Experiment Overall Design: Genomic DNA from a MMTV-cdc25a;MMTV-neu double transgenic murine cell line derived from a mammary tumor was compared to normal mammary tissue DNA from the parental strain. A total of two hybridizations were completed.
|Date made available||2008|