Description
Accession Number: GSE44939
Platform:
GPL16288: AB 5500xl Genetic Analyzer (Homo sapiens)
Organism: Homo sapiens
Published on 2013-08-28
Summary:
We report the application of high throughput sequencing technology for investigating the transcriptional regulatory network of the human innate immune response. With ChIP-seq, we generated genome-wide virus-activated transcription factor and transcription machinery maps of infected and uninfected human Namalwa B cells. Analysis of ChIP-seq data reveals extensive collaboration of IRF3 and NF-κB with Mediator throughout the human genome, and implicates additional transcription factor partners in antiviral responses. Moreover, analysis of Pol II occupancy and elongation status during virus infection indicates that IRF3 and NF-κB drive both de novo polymerase recruitment and mediate polymerase pause-release at their target sites, stimulating the expression of a variety of protein-coding, non-coding, and unannotated loci.
Overall Design:
Examination of 6 different proteins before and after virus infection in 1 cell type.
Contact:
Name: Jonathan E Freaney
Organization: Northwestern University
Address: 2200 Campus Drive Evanston Illinois 60208 USA
Email: jfreaney@u.northwestern.edu
Organization: GEO
Address: USA
Platform:
GPL16288: AB 5500xl Genetic Analyzer (Homo sapiens)
Organism: Homo sapiens
Published on 2013-08-28
Summary:
We report the application of high throughput sequencing technology for investigating the transcriptional regulatory network of the human innate immune response. With ChIP-seq, we generated genome-wide virus-activated transcription factor and transcription machinery maps of infected and uninfected human Namalwa B cells. Analysis of ChIP-seq data reveals extensive collaboration of IRF3 and NF-κB with Mediator throughout the human genome, and implicates additional transcription factor partners in antiviral responses. Moreover, analysis of Pol II occupancy and elongation status during virus infection indicates that IRF3 and NF-κB drive both de novo polymerase recruitment and mediate polymerase pause-release at their target sites, stimulating the expression of a variety of protein-coding, non-coding, and unannotated loci.
Overall Design:
Examination of 6 different proteins before and after virus infection in 1 cell type.
Contact:
Name: Jonathan E Freaney
Organization: Northwestern University
Address: 2200 Campus Drive Evanston Illinois 60208 USA
Email: jfreaney@u.northwestern.edu
Organization: GEO
Address: USA
| Date made available | 2013 |
|---|---|
| Publisher | Gene Expression Omnibus |