Description
Accession Number: GSE103234
Platform:
GPL11203: Illumina Genome Analyzer IIx (Drosophila melanogaster)
Organism: Drosophila melanogaster
Published on 2017-09-15
Summary:
The cellular abundance of mature microRNAs (miRNAs) is dictated by the efficiency of nuclear processing of primary miRNA transcripts (pri-miRNAs) into pre-miRNA intermediates. The Microprocessor complex of Drosha and DGCR8 carries this out, but it has been unclear what controls Microprocessor's differential processing of various pri-miRNAs. Here, we show that Drosophila DGCR8 (Pasha) directly associates with the C terminal domain of the RNA polymerase II elongation complex when it is phosphorylated by the Cdk9 kinase (pTEFb). When association is blocked by loss of Cdk9 activity, a global change in pri-miRNA processing is detected. Processing of pri-miRNAs with a UGU sequence motif in their apical junction domain increases, while processing of pri-miRNAs lacking this motif decreases. Therefore, phosphorylation of RNA polymerase II recruits Microprocessor for co-transcriptional processing of non-UGU pri-miRNAs that would otherwise be poorly processed. In contrast, UGU-positive pri-miRNAs are robustly processed by Microprocessor independent of RNA polymerase association.
Overall Design:
There are 2 samples, one control and one experimental
Contact:
Name: Richard W. Carthew
Organization: Northwestern University
Deparment: Molecular Biosciences
Address: 2153 North Campus Drive, Hogan Hall Room 4-120 Evanston IL 60208-3500 USA
Email: [email protected]
Phone: 847-467-4891
Organization: GEO
Address: USA
Platform:
GPL11203: Illumina Genome Analyzer IIx (Drosophila melanogaster)
Organism: Drosophila melanogaster
Published on 2017-09-15
Summary:
The cellular abundance of mature microRNAs (miRNAs) is dictated by the efficiency of nuclear processing of primary miRNA transcripts (pri-miRNAs) into pre-miRNA intermediates. The Microprocessor complex of Drosha and DGCR8 carries this out, but it has been unclear what controls Microprocessor's differential processing of various pri-miRNAs. Here, we show that Drosophila DGCR8 (Pasha) directly associates with the C terminal domain of the RNA polymerase II elongation complex when it is phosphorylated by the Cdk9 kinase (pTEFb). When association is blocked by loss of Cdk9 activity, a global change in pri-miRNA processing is detected. Processing of pri-miRNAs with a UGU sequence motif in their apical junction domain increases, while processing of pri-miRNAs lacking this motif decreases. Therefore, phosphorylation of RNA polymerase II recruits Microprocessor for co-transcriptional processing of non-UGU pri-miRNAs that would otherwise be poorly processed. In contrast, UGU-positive pri-miRNAs are robustly processed by Microprocessor independent of RNA polymerase association.
Overall Design:
There are 2 samples, one control and one experimental
Contact:
Name: Richard W. Carthew
Organization: Northwestern University
Deparment: Molecular Biosciences
Address: 2153 North Campus Drive, Hogan Hall Room 4-120 Evanston IL 60208-3500 USA
Email: [email protected]
Phone: 847-467-4891
Organization: GEO
Address: USA
Date made available | Aug 29 2017 |
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Publisher | Gene Expression Omnibus |