Mouse Hepatocellular Carcinoma Study

  • Ju Seog Lee (Contributor)
  • In Sun Chu (Contributor)
  • Arsen Mikaelyan (Contributor)
  • Diego F. Calvisi (Contributor)
  • Jeonghoon Heo (Contributor)
  • Janardan K. Reddy (Contributor)
  • Snorri S. Thorgeirsson (Contributor)



Accession Number: GSE1897


Organism: Mus musculus

Published on 2004-10-28

Genetically modified mice have been extensively used for analyzing the molecular events that occur during the tumor development. However, in many cases, if not all, it is uncertain to what extent the mouse models reproduce features observed in the corresponding human conditions. This is due largely to lack of precise methods for direct and comprehensive comparison at the molecular level of the mouse and human tumors. We used global gene expression patterns of 68 hepatocellular carcinoma (HCC) from 7 different mouse models and 91 human HCC from pre-defined subclasses to obtain direct comparison of the molecular features of mouse and human HCC. Total RNAs were isolated from frozen liver tissue using CsCl density gradient centrifugation methods. Total RNA from the livers of 10 wild type mice were pooled and used as reference in entire microarray experiments. To obtain gene expression profile data from four transgenic HCC mouse models, 20 μg of total RNAs from tissues were used to drive fluorescently (Cy-5 or Cy-3) labeled cDNA. At least two hybridizations were carried out for each tissue using dye-swap strategy to eliminate dye labeling bias. Keywords: other

Name: Snorri S Thorgeirsson
Organization: National Cancer institute/NIH
Laboratory: Cellular and Molecular Biology Section
Deparment: Laboratory of Experimental Carcinogenesis
Address: 37 Convent Dr. # 4146 Bethesda MD 20892 USA
Email: [email protected]
Phone: (301) 496-5688 x204

Name: Lisa M Gangi
Organization: NCI
Laboratory: Microarray Research Facility
Deparment: LMT-Frederick
Address: 915 Tollhouse Ave Frederick MD 21701 USA
Email: [email protected]
Phone: 301-846-6296
Date made availableOct 27 2004
PublisherGene Expression Omnibus

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