BioCryo (Biological and Cryogenic Electron Microscopy) Facility

    Equipment/facility: Facility

    • Location

      Hogan Hall, 5-150 2205 Tech Drive Evanston, IL 60208

      United States

    Description

    The integrated BioCryo Facility provides a comprehensive source for characterization of biological, soft matter and hybrid samples with TEM (Transmission Electron Microscopy), SEM (Scanning Electron Microscopy), and STEM (Scanning Transmission Electron Microscopy) at cryogenic or at ambient temperature. This facility provides expertise and equipment for cryofixation and processing of frozen hydrated samples by high-pressure freezing, plunge freezing, cryo fracturing, cryo ultramicrotomy, freeze substitution, and turbo freeze-drying. Conventional samples can be processed at ambient temperature with chemical fixation, dehydration, and resin embedding, or critical point drying. The BioCryo Facility also offers analytical STEM with EDS (Energy Dispersive X-ray Spectroscopy) and EELS (Electron Energy Loss Spectroscopy), serial sectioning for 3D reconstruction, and immunolabeling, as well as image processing. This repertoire of capabilities is constantly expanded by implementation of additional innovative instrumentation and techniques into BioCryo workflows. In addition to offering consultation and training on instruments and sample preparation techniques, BioCryo staff mentors new users, advises on experimental design, and collaborates with researchers in experiments with challenging and unconventional samples. Similar to the other NUANCE facilities (EPIC, Keck-II, SPID), the BioCryo Facility participates in numerous educational and outreach events, e.g. workshops, seminars, demos, and facility tours.

    Details

    NameBioCryo
    Acquisition date1/3/18

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    cryogenics
    electron microscopy
    freezing
    transmission electron microscopy
    scanning electron microscopy
    ambient temperature
    freeze drying
    fracturing
    dehydration
    embedding
    resins
    spectroscopy
    drying
    image processing
    critical point
    education
    energy dissipation
    electron energy
    substitutes
    preparation

    Categories

    • Chemical Mapping
    • Critical point drying
    • cryo SEM
    • cryo STEM
    • cryo TEM
    • Cryo/Low-Temp Preparation
    • Electron Microscopy
    • High–pressure freezing
    • Image Analysis
    • Imaging
    • Life science imaging
    • Sample Preparation
    • Scanning electron microscopy (SEM)
    • Scanning transmission electron microscopy (STEM)
    • Specimen Preparation
    • Specimen Preparation Facility Equipment
    • Training
    • Transmission electron microscopy (TEM)
    • Ultramicrotomy

    Resources

    • Hitachi HD-2300A Dual EDS Cryo S/TEM
    • Hitachi HT-7700 Biological S/TEM
    • Hitachi S4800-II cFEG SEM
    • JEOL 1230 TEM
    • High Pressure Freezer HPM100 (Leica)
    • FEI Vitrobot Plunge Freezer
    • Leica UC7/FC7 Cryo-Ultramicrotome
    • Leica EM ACE600 High Vacuum Coater
    • BioCryo Leica EM AFS2 Freeze Substitution & Low Temperature Embedding System
    • BioCryo Tousimis Critical Point Dryer (Samdri-795)
    • BioCryo VT1200 S Vibrating-blade Microtome
    • BioCryo Cressington 208HR Sputter Coater