Biological Imaging Facility

Equipment/facility: Facility

Description

The Biological Imaging Facility (BIF) is a shared-use research and training resource available to all NU researchers. BIF is organized so users can prepare samples, capture and analyze images, and create final presentations in one facility. Training for all instruments is available on a regular basis so users can acquire data quickly and efficiently. We are continuously looking for new ways to enhance existing equipment, acquire new tools, and keep pace with current techniques.

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Imaging techniques

Categories

  • Cryo/Low-Temp Preparation
  • Electron Microscopy
  • High–pressure freezing
  • Imaging
  • Specimen Preparation
  • Specimen Preparation Facility Equipment
  • Transmission electron microscopy (TEM)
  • Ultramicrotomy
  • Light Microscopy
  • Consultation
  • Training
  • Confocal Microscopy
  • Fluorescence/Photonic Microscopy
  • Image Analysis
  • Imaging Software
  • Life science imaging
  • Critical point drying
  • cryo TEM

Resources

  • Cryoultramicrotomy
  • cryosectioning
  • ImageJ
  • MetaMorph
  • Applied Precision DeltaVision Core Microscope System
  • Cressington 626 Cryotransfer System
  • Cressington Quick Freeze Deep Etch (QFDE) - Freeze-Fracture
  • Cressington Sputter Coater
  • Denton Vacuum Evaporator
  • FEI Vitrobot - plunge-freezing
  • HP DesignJet 5500 PS large format printer for posters
  • JEOL 1230 120 kV Transmission Electron Microscope - TEM
  • cryoTEM
  • Leica AFS2 Automatic Freeze Substitution - FS
  • Leica EMPACT2 High Pressure Freezer - HPF
  • Leica MM80 Impact Freezer
  • slam-freezing
  • metal mirror
  • Leica SP2 Laser Scanning Confocal Microscope - LSCM
  • Leica SP5 Laser Scanning Confocal Microscope
  • Leica Ultracut S Ultramicrotome - with FCS cryoultramicrotomy module
  • Leica Widefield Fluorescence Microscope
  • Pelco BioWave Microwave
  • Polaron Critical Point Dryer
  • RMC 6000 Ultramicrotome
  • RMC PT-PC Ultramicrotome
  • Zeiss 510 Meta/ConfoCor3 Laser Scanning Confocal Microscope - LSCM
  • FCS
  • Adobe Creative Suite - Photoshop
  • Illustrator
  • InDesign
  • Acrobat
  • Fluorescence Correlation Spectroscopy - FCS
  • ImageProPlus
  • Molecular Devices MetaMorph Image Analytical Software
  • Networked Mac or PC computer workstations with CD/DVD drive
  • USB drive
  • Firewire drive
  • Training on BIF instruments
  • Volocity 3D Image Analysis Software