Center for Advanced Microscopy and Nikon Imaging Center

Equipment/facility: Facility

Description

The Center for Advanced Microscopy (CAM) at Northwestern offers state-of-the art instrumentation and services for the study of biological processes at the whole animal, tissue, cellular and subcellular levels. The facility's basic services include electron microscopy, super resolution microscopy (SIM & STORM), fluorescent laser scanning and spinning disk microscopy, fluorescent lifetime imaging, fluorescence correlation spectroscopy microscopy, automated high throughput tissue cytometry, laser capture microdissection, mutliphoton imaging, and whole animal bioluminescent and fluorescent imaging. Additionally we provide microinjection equipment, chambers for stable live cell observation, and anesthesia equipment. CAM staff members provide training on numerous different instrument platforms, consultation on experiment design, as well as digital image processing and image analysis. We are a reference site for several companies including TissueGnostics and ISS (developer of the ISS ALBA). CAM is one of three Nikon Imaging Centers in the US, allowing us access and excellent support from Nikon to develop innovative solutions for the cutting edge imaging needs of users. This relationship has allowed our users to push super-resolution technologies to permit multi-color, 3D and live-cell applications. Additionally, we have been working together with Nikon to add confocal laser scanning to a low magnification upright dissection microscope, which is particularly useful to researchers interested in visualizing thick samples (such as epidermal raft cultures) at increased cellular resolution. We are also currently expanding our full service electron microscopy offerings. Recently, we obtained a Leica Freeze Substitution Unit for TEM sample preparation allowing for improved sample quality and streamlined work-flow. Please feel free to contact us with questions.

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Microscopic examination
Microscopy
Imaging techniques
Lasers
Electron Microscopy
Equipment and Supplies
Electron microscopy
Animals
Tissue
Scanning
Freeze Substitution
Laser Capture Microdissection
Workflow
Fluorescence Spectrometry
Microinjections
Biological Processes
Dissection
Referral and Consultation
Anesthesia
Color

Categories

  • Electron Microscopy
  • Imaging
  • Transmission electron microscopy (TEM)
  • Ultramicrotomy
  • Light Microscopy
  • Training
  • Confocal Microscopy
  • Fluorescence/Photonic Microscopy
  • Image Analysis
  • Imaging Software
  • In vivo Animal Imaging
  • Two-Photon Imaging

Resources

  • Fluorescence-based non-invasive small animal imaging
  • IVIS Spectrum in vivo bioluminescence/fluorescence imager
  • Zeiss LSM 510 META laser scanning confocals (two systems)
  • Nikon/Yokogawa spinning disc confocal
  • TissueGnostics Tissue/Cell High Throughput Imaging and Analysis System
  • Olympus DSU spinning disc confocal
  • Zeiss Axioskop w/ CRi Nuance spectral camera
  • Eppendorf FemtoJet injector on Zeiss Axiovert fluorescence microscope
  • Leica UC6 Ultramicrotome
  • Leica Ultracut UCT Ultramicrotome
  • Leica Ultracut E Ultramicrotome
  • Baltec Rotary Shadower
  • Zeiss LSM software
  • ImageJ
  • MetaMorph
  • AutoQuant X for deconvolution
  • Photoshop
  • TissueQuest
  • HistoQuest
  • FRAP-FLIP Calc
  • FEI Tecnai Spirit G2 transmission electron microscope
  • Nikon Biostation Incubator Microscope
  • Perkin Elmer Xenogen IVIS Spectrum Bioluminescence System
  • Total Internal Reflection Fluorescence (TIRF)
  • Nikon C2 Laser Scanning Confocal
  • Nikon A1R Laser Scanning Confocal
  • Nikon A1 MP Laser Scanning Confocal
  • Nikon AZ100 Fluorescent Dissection Microscope
  • HP DesignJet 60 inch Poster Printer
  • Nikon N-SIM Structured Illumination Microscope
  • Nikon Biostation
  • ISS Alba FCS FLIM Anisotropy Microscope
  • Nikon N-Storm Stochastic Optical Reconstruction Microscope
  • FRET
  • FRAP
  • Volocity
  • Microinjection
  • Needle puller
  • Electron microscopy of tissue and cultured cell specimens
  • Full service sample preparation and sectioning
  • Critical point drying
  • Immunogold EM
  • Platinum-replica electron microscopy
  • Correlative light and electron microscopy CLEM
  • Sputter-coating
  • Digital and conventional EM image acquisition
  • Fluorescence-based minimally invasive small animal intravital imaging
  • Individual training on each instrument
  • bioluminescence
  • Poster Printing