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Personal profile

Research Interests

Cilia Polarity. The ability of ciliated epithelia to generate directed fluid flow is an important aspect of diverse developmental and physiological processes including proper respiratory function. To achieve directed flow, ciliated cells must generate 100-200 cilia that are polarized along a common axis both within and between cells. My lab is currently working towards understanding the molecular mechanisms for how cell polarity is coordinated as well as how individual cilia interpret the cells polarity. Centriole Duplication. Centrioles are microtubule based structures with nine fold symmetry that are involved in both centrosome organization and aster formation during cell division. During the normal cell cycle centrioles duplicate once, generating a mother / daughter pair, and in most post-mitotic vertebrate cells the mother centriole then goes on to form the basal body of a sensory cilium. Abnormalities in the duplication of centrioles (and centrosomes) are prevalent in many cancers suggesting a link between centriole duplication and cancer progression. My laboratory is addressing this fundamental question in cell biology from a novel direction with the use of Xenopus motile ciliated cells. Ciliated cells are unique among vertebrate cells in that they naturally generate hundreds of centrioles therefore providing a great system for studying the regulation of centriole duplication. Understanding how nature has overcome the typically tight regulation of centriole duplication will lend insight into the molecular mechanisms of cancer progression.

Education/Academic qualification

PhD, University of North Carolina

… → 2001

Keywords

  • Cancer Biology
  • Cell Biology
  • Cell Imaging
  • Developmental Biology
  • Respiratory Diseases

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  • 5 Similar Profiles
Cilia Medicine & Life Sciences
Cell Polarity Medicine & Life Sciences
Centrioles Medicine & Life Sciences
Xenopus Medicine & Life Sciences
Microtubules Medicine & Life Sciences
Basal Bodies Medicine & Life Sciences
Epithelium Medicine & Life Sciences
Kartagener Syndrome Medicine & Life Sciences

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Grants 2008 2021

Microtubules
Epithelium
Biological Phenomena
Neoplasms
Molecular Imaging
Equipment and Supplies
Microtubules
Epithelium
Biological Phenomena
Neoplasms
Cilia
Centrioles
Epithelium
Cues
Xenopus
Cilia
Centrioles
Epithelium
Equipment and Supplies
Cues

Research Output 2002 2019

Lack of GAS2L2 Causes PCD by Impairing Cilia Orientation and Mucociliary Clearance

Bustamante-Marin, X. M., Yin, W. N., Sears, P. R., Werner, M. E., Brotslaw, E. J., Mitchell, B. J., Jania, C. M., Zeman, K. L., Rogers, T. D., Herring, L. E., Refabért, L., Thomas, L., Amselem, S., Escudier, E., Legendre, M., Grubb, B. R., Knowles, M. R., Zariwala, M. A. & Ostrowski, L. E., Feb 7 2019, In : American Journal of Human Genetics. 104, 2, p. 229-245 17 p.

Research output: Contribution to journalArticle

Kartagener Syndrome
Mucociliary Clearance
Cilia
Growth
Proteins
6 Citations (Scopus)

CLA MP/Spef1 regulates planar cell polarity signaling and asymmetric microtubule accumulation in the Xenopus ciliated epithelia

Kim, S. K., Zhang, S., Werner, M. E., Brotslaw, E. J., Mitchell, J. W., Altabbaa, M. M. & Mitchell, B. J., May 1 2018, In : Journal of Cell Biology. 217, 5, p. 1633-1641 9 p.

Research output: Contribution to journalArticle

Cell Polarity
Xenopus
Microtubules
Epithelium
Cilia
4 Citations (Scopus)

Epha2/ephrin-A1 mediate corneal epithelial cell compartmentalization via ADAM10 regulation of EGFR signaling

Kaplan, N., Ventrella, R., Peng, H., Pal-Ghosh, S., Arvanitis, C., Rappoport, J. Z., Mitchell, B. J., Stepp, M. A., Lavker, R. M. & Getsios, S., Jan 1 2018, In : Investigative Ophthalmology and Visual Science. 59, 1, p. 393-406 14 p.

Research output: Contribution to journalArticle

Ephrin-A1
Epidermal Growth Factor Receptor
Epithelial Cells
Corneal Epithelium
EphA2 Receptor

The small molecule AMBMP disrupts microtubule growth, ciliogenesis, cell polarity, and cell migration

Werner, M., del Castillo, U., Ventrella, R., Brotslaw, E. & Mitchell, B. J., Oct 1 2018, In : Cytoskeleton. 75, 10, p. 450-457 8 p.

Research output: Contribution to journalArticle

Cell Polarity
Microtubules
Cell Movement
Growth
Nocodazole
5 Citations (Scopus)

Basal bodies in Xenopus

Zhang, S. & Mitchell, B. J., Feb 3 2016, In : Cilia. 5, 1, 2.

Research output: Contribution to journalReview article

Basal Bodies
Xenopus
Clustered Regularly Interspaced Short Palindromic Repeats
Cilia
Transmission Electron Microscopy