• Source: Scopus
20062021

Research activity per year

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Personal profile

Research Interests

Faculty Member, Center for Synthetic Biology We develop high-throughput methods for protein biophysics and protein design, with a focus on protein therapeutics. Key questions include: How do protein sequence and structure determine folding stability, conformational dynamics, and resistance to aggregation/degradation-inducing stresses? Can we quantitatively predict these protein "phenotypes" from genotype (sequence) using computational modeling? How do we design protein therapeutics that optimize these phenotypes for a particular application? To answer these questions, we combine large-scale de novo computational protein design with high-throughput methods such as display selections, mass spectrometry proteomics, and next-generation sequencing, enabling us to test thousands of proteins in parallel. By combining these technologies, we seek to develop efficient "design-test-analyze" cycles, iterating our way to an improved, quantitative understanding of protein biophysics and more advanced protein therapeutics.

Training Experience

2019Postdoctoral Fellowship, University of Washington, Institute for Protein Design

Education/Academic qualification

Biophysics, PhD, University of California, San Francisco

… → 2013

Research interests

  • Biochemistry: Proteins
  • Bioengineering
  • Biologic Therapies
  • Biophysics
  • Computational Biology
  • Drug Discovery
  • Proteins / Macromolecules
  • Proteomics

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  • The influence of proline isomerization on potency and stability of anti-HIV antibody 10E8

    Guttman, M., Padte, N. N., Huang, Y., Yu, J., Rocklin, G. J., Weitzner, B. D., Scian, M., Ho, D. D. & Lee, K. K., Dec 1 2020, In: Scientific reports. 10, 1, 14313.

    Research output: Contribution to journalArticlepeer-review

    Open Access
    2 Scopus citations
  • Global analysis of protein folding using massively parallel design, synthesis, and testing

    Rocklin, G., Chidyausiku, T. M., Goreshnik, I., Ford, A., Houliston, S., Lemak, A., Carter, L., Ravichandran, R., Mulligan, V. K., Chevalier, A., Arrowsmith, C. H. & Baker, D., Jul 14 2017, In: Science. 357, 6347, p. 168-175 8 p.

    Research output: Contribution to journalArticlepeer-review

    Open Access
    164 Scopus citations
  • Massively parallel de novo protein design for targeted therapeutics

    Chevalier, A., Silva, D. A., Rocklin, G., Hicks, D. R., Vergara, R., Murapa, P., Bernard, S. M., Zhang, L., Lam, K. H., Yao, G., Bahl, C. D., Miyashita, S. I., Goreshnik, I., Fuller, J. T., Koday, M. T., Jenkins, C. M., Colvin, T., Carter, L., Bohn, A., Bryan, C. M. & 8 others, Fernández-Velasco, D. A., Stewart, L., Dong, M., Huang, X., Jin, R., Wilson, I. A., Fuller, D. H. & Baker, D., Oct 5 2017, In: Nature. 550, 7674, p. 74-79 6 p.

    Research output: Contribution to journalArticlepeer-review

    163 Scopus citations
  • Accurate de novo design of hyperstable constrained peptides

    Bhardwaj, G., Mulligan, V. K., Bahl, C. D., Gilmore, J. M., Harvey, P. J., Cheneval, O., Buchko, G. W., Pulavarti, S. V. S. R. K., Kaas, Q., Eletsky, A., Huang, P. S., Johnsen, W. A., Greisen, P. J., Rocklin, G., Song, Y., Linsky, T. W., Watkins, A., Rettie, S. A., Xu, X., Carter, L. P. & 7 others, Bonneau, R., Olson, J. M., Coutsias, E., Correnti, C. E., Szyperski, T., Craik, D. J. & Baker, D., 2016, In: Nature. 538, 7625, p. 329-335 7 p.

    Research output: Contribution to journalArticlepeer-review

    169 Scopus citations
  • Blind prediction of charged ligand binding affinities in a model binding site

    Rocklin, G., Boyce, S. E., Fischer, M., Fish, I., Mobley, D. L., Shoichet, B. K. & Dill, K. A., Nov 15 2013, In: Journal of Molecular Biology. 425, 22, p. 4569-4583 15 p.

    Research output: Contribution to journalArticlepeer-review

    36 Scopus citations