Grants per year
Personal profile
Research Interests
Our laboratory studies the neuroinvasive herpesviruses with a focus on the molecular mechanisms by which these pathogens propagate and disseminate within the nervous system. The outcome of infection is closely tied to the route the viruses spread within the nervous system, and can range from no overt disease to blindness or lethal encephalitis. We use a combination of live-cell fluorescence microscopy, molecular genetics and neuronal cell biology to examine the dynamics of viral particle assembly and intracellular axon transport. These molecular mechanisms are then examined in the larger context of neuroinvasion and pathogenesis. Two long-term goals of our research are to identify novel targets in the viral infectious cycle for development of antivirals, and engineering recombinant viral particles as effective gene delivery vehicles. (Keywords: neuroinvasion, neuron, axon, microtubule, cytoskeleton, dynein, kinesin, transport, virus, virion, herpesvirus, herpes, capsid, tegument, egress, nucleus, GFP, BAC, bacmid, recombination, GS1783)
Training Experience
2001 | Postdoctoral Fellowship, Princeton University |
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
Education/Academic qualification
PhD, Microbiology, University of Pennsylvania
… → 1996
Research interests keywords
- Cell Biology
- Microbiology
- Microscopy
- Molecular Biology
- Molecular Genetics
- Neuroscience
- Viral Pathogenesis
- Virology
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Collaborations and top research areas from the last five years
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Production and assessment of a UL37R2 attenuated Marek’s Disease Virus
Smith, G. A. (PD/PI) & Smith, G. A. (PD/PI)
6/12/23 → 6/30/25
Project: Research project
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Neurotropic herpesvirus envelopment and microtubule-mediated transport
Smith, G. A. (PD/PI)
Albert Einstein College of Medicine, National Institute of Allergy and Infectious Diseases
12/1/22 → 11/30/27
Project: Research project
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ALPHA-HERPESVIRUS TRANSPORT IN AXONS
Smith, G. A. (PD/PI), Smith, G. A. (PD/PI) & Smith, G. A. (PD/PI)
National Institute of Allergy and Infectious Diseases
7/1/20 → 6/30/26
Project: Research project
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Virus-host interactions governing alpha-herpesvirus genome delivery and neuroinvasion
Smith, G. A. (PD/PI), Smith, G. A. (PD/PI) & Smith, G. A. (PD/PI)
National Institute of Allergy and Infectious Diseases
2/1/20 → 1/31/26
Project: Research project
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Pseudorabies virus infection triggers pUL46-mediated phosphorylation of connexin-43 and closure of gap junctions to promote intercellular virus spread
Tishchenko, A., Romero, N., van Waesberghe, C., Delva, J. L., Vickman, O., Smith, G. A., Mettenleiter, T. C., Fuchs, W., Klupp, B. G. & Favoreel, H. W., Jan 2025, In: PLoS pathogens. 21, 1, e1012895.Research output: Contribution to journal › Article › peer-review
Open Access -
Tethered release of the pseudorabies virus deubiquitinase from the capsid promotes enzymatic activity
Antinone, S. E., Miller, J. S., Huffmaster, N. J., Pickard, G. E. & Smith, G. A., Jan 2025, In: Journal of virology. 99, 1Research output: Contribution to journal › Article › peer-review
Open Access -
The gE/gI complex is necessary for kinesin-1 recruitment during alphaherpesvirus egress from neurons
Diwaker, D., Kim, D. H., Cordova-Martinez, D., Pujari, N., Jordan, B. A., Smith, G. A. & Wilson, D. W., Jan 2025, In: Journal of virology. 99, 1Research output: Contribution to journal › Article › peer-review
Open Access -
Human TMEFF1 is a restriction factor for herpes simplex virus in the brain
Chan, Y. H., Liu, Z., Bastard, P., Khobrekar, N., Hutchison, K. M., Yamazaki, Y., Fan, Q., Matuozzo, D., Harschnitz, O., Kerrouche, N., Nakajima, K., Amin, P., Yatim, A., Rinchai, D., Chen, J., Zhang, P., Ciceri, G., Chen, J., Dobbs, K. & Belkaya, S. & 29 others, , Aug 8 2024, In: Nature. 632, 8024, p. 390-400 11 p.Research output: Contribution to journal › Article › peer-review
Open Access6 Scopus citations -
Leveraging biotin-based proximity labeling to identify cellular factors governing early alphaherpesvirus infection
Quan, J., Fan, Q., Simons, L. M., Smukowski, S. N., Pegg, C., Longnecker, R., Savas, J. N., Hultquist, J. F. & Smith, G. A., Aug 2024, In: mBio. 15, 8Research output: Contribution to journal › Article › peer-review
Open Access3 Scopus citations
Datasets
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Crystal Structure of N-terminal half of Pseudorabiesvirus UL37 protein
Pitts, J. D. (Contributor), Klabis, J. (Contributor), Richards, A. L. (Contributor), Smith, G. A. (Contributor) & Heldwein, E. E. (Contributor), Protein Data Bank (PDB), Mar 19 2014
DOI: 10.2210/pdb4K70/pdb, https://www.wwpdb.org/pdb?id=pdb_00004k70
Dataset
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PRV UL37 N-terminal half (R2 mutant)
Richards, A. L. (Contributor), Sollars, P. J. (Contributor), Pitts, J. D. (Contributor), Stults, A. M. (Contributor), Heldwein, E. E. (Contributor), Pickard, G. E. (Contributor) & Smith, G. A. (Contributor), Protein Data Bank (PDB), Oct 4 2017
DOI: 10.2210/pdb5J2Z/pdb, https://www.wwpdb.org/pdb?id=pdb_00005j2z
Dataset
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Additional file 2: of New tools to convert bacterial artificial chromosomes to a self-excising design and their application to a herpes simplex virus type 1 infectious clone
Richards, A. L. (Contributor), Sollars, P. J. (Creator) & Smith, G. A. (Creator), figshare, 2016
DOI: 10.6084/m9.figshare.c.3639116_d2, https://springernature.figshare.com/articles/dataset/Additional_file_2_of_New_tools_to_convert_bacterial_artificial_chromosomes_to_a_self-excising_design_and_their_application_to_a_herpes_simplex_virus_type_1_infectious_clone/4451186
Dataset
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New tools to convert bacterial artificial chromosomes to a self-excising design and their application to a herpes simplex virus type 1 infectious clone
Richards, A. L. (Contributor), Sollars, P. J. (Creator) & Smith, G. A. (Creator), figshare, 2016
DOI: 10.6084/m9.figshare.c.3639116, https://figshare.com/collections/New_tools_to_convert_bacterial_artificial_chromosomes_to_a_self-excising_design_and_their_application_to_a_herpes_simplex_virus_type_1_infectious_clone/3639116
Dataset