Gregory B Waypa

  • 2050 Citations
19962020
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Personal profile

Research Interests

Gregory B. Waypa, Ph.D. received his B.S. in Biomedical Engineering Sciences from the University of California, San Diego in 1991. He then enrolled as a graduate student at Albany Medical College in Albany, New York, where, under Fred L. Minnear, Ph.D., he studied increased pulmonary vascular permeability associated acute respiratory distress syndrome (ARDS) and explored the possibility of using cAMP-enhancing agents as a possible therapy for the disease. Dr. Waypa received his M.S., and later his Ph.D., in Physiology and Cell Biology from Albany Medical College in 1997 and 1998, respectively. From 1998 to 2002, Dr. Waypa was a postdoctoral fellow with Paul T. Schumacker, Ph.D. at The University of Chicago. In 2002, Dr. Waypa received an appointment from The University of Chicago as a Research Associate (Assistant Professor) where he continued to work with Dr. Schumacker. In 2004, Dr. Waypa received an appointment from Northwestern University as a Research Assistant Professor where he is continuing his collaboration with Dr. Schumacker. In 2015, Dr. Waypa was promoted to Research Associate Professor in the Department of Pediatrics and Neonatology. Currently, Dr. Waypa is studying hypoxic pulmonary vasoconstriction (HPV), which is a physiological response to low alveolar oxygen tension and, in cases where a small percentage of the lung alveoli are hypoxic, HPV improves lung gas exchange by redistributing blood flow away from those areas toward regions with better oxygenation. Key to this research is understanding the mechanism of O2 sensing by the pulmonary vasculature and how this results in the generation of the HPV response.

None.

Currently, I am studying acute and chronic hypoxic pulmonary vasoconstriction (HPV), a physiological response where pulmonary arteries constrict in response to alveolar hypoxia. During chronic alveolar hypoxia, this pulmonary vasoconstriction can result in the development of pulmonary hypertension, right ventricular hypertrophy, cor pulmonale, and death. An important unresolved question in this field relates to the underlying mechanism by which the vascular cells detect a decrease in oxygen tension and translate that into a signal that triggers the functional response. I have been collaborating with Dr. Schumacker in testing the hypothesis that the mitochondria function as O2 sensors by initiating a reactive oxygen species signaling pathway that triggers the HPV response. I have focused my efforts on the use of imaging methodology to address this question, using ratiometric, genetically encoded fluorescent sensors (roGFP) targeted to subcellular compartments including the cytosol, mitochondrial matrix, and intermembrane space of mitochondria. I have also used genetically encoded, ratiometric calcium sensors targeted to the cytosol or the mitochondrial matrix to assess calcium signaling using imaging techniques. I am currently extending our imaging technology by engineering transgenic mice expressing cell-specific, targeted roGFP. By employing these mice, we will be able to induce the expression of the roGFP in endothelial cells or in smooth muscle cells. In addition, I’ve begun to measure changes in pulmonary hemodynamics in mice challenged with acute and chronic hypoxia. We will use these approaches to assess changes in pulmonary hemodynamics in whole mice and redox and calcium signaling in vascular cells in precision cut lung slices as well as in intact lungs of mice subjected to acute hypoxia, chronic hypoxia, or control conditions.

Education/Academic qualification

PhD, Albany Medical College

… → 1998

Research interests

  • Angiogenesis
  • Apoptosis
  • Bioengineering
  • Cell Biology
  • Circulation
  • Molecular Biology
  • Physiology
  • Signal Transduction

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  • 14 Similar Profiles
Reactive Oxygen Species Medicine & Life Sciences
Lung Medicine & Life Sciences
Vasoconstriction Medicine & Life Sciences
Oxidants Medicine & Life Sciences
Pulmonary Artery Medicine & Life Sciences
Smooth Muscle Myocytes Medicine & Life Sciences
Mitochondria Medicine & Life Sciences
Oxidation-Reduction Medicine & Life Sciences

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Grants 2005 2020

Pulmonary Hypertension
Lung
Pulmonary Artery
Oxidation-Reduction
Smooth Muscle Myocytes
Vasoconstriction
Reactive Oxygen Species
Lung
Electron Transport
Cytosol
Cell Hypoxia
Hypoxia-Inducible Factor 1
Reactive Oxygen Species
Mitochondria
Prolyl Hydroxylases

Research Output 1996 2018

  • 2050 Citations
  • 23 Article
  • 4 Review article
  • 1 Chapter
  • 1 Conference contribution
4 Citations (Scopus)

JNK2 up-regulates hypoxia-inducible factors and contributes to hypoxia-induced erythropoiesis and pulmonary hypertension

Sala, M. A., Chen, C., Zhang, Q., Do-Umehara, H. C., Wu, W., Misharin, A. V., Waypa, G. B., Fang, D., Budinger, G. R. S., Liu, S., Chandel, N. S., Schumacker, P. T., Sznajder, J. I. & Liu, J., Jan 1 2018, In : Journal of Biological Chemistry. 293, 1, p. 271-284 14 p.

Research output: Contribution to journalArticle

Hypoxia-Inducible Factor 1
Erythropoiesis
Pulmonary Hypertension
Up-Regulation
Protein Kinases

Aberrant cGMP signaling persists during recovery in mice with oxygen-induced pulmonary hypertension

Perez, M., Lee, K. J., Cardona, H. J., Taylor, J. M., Robbins, M. E., Waypa, G. B., Berkelhamer, S. K. & Farrow, K. N., Jan 1 2017, In : PloS one. 12, 8, p. e0180957

Research output: Contribution to journalArticle

bronchopulmonary dysplasia
guanosine monophosphate
Bronchopulmonary Dysplasia
Cyclic GMP
Recovery Room
36 Citations (Scopus)

Redox signaling during hypoxia in mammalian cells

Smith, K. A., Waypa, G. B. & Schumacker, P. T., Oct 1 2017, In : Redox Biology. 13, p. 228-234 7 p.

Research output: Contribution to journalReview article

Oxidation-Reduction
Reactive Oxygen Species
Cells
Mitochondria
NADPH Oxidase
52 Citations (Scopus)

O2 sensing, mitochondria and ROS signaling: The fog is lifting

Waypa, G. B., Smith, K. A. & Schumacker, P. T., Feb 1 2016, In : Molecular Aspects of Medicine. 47-48, p. 76-89 14 p.

Research output: Contribution to journalReview article

Mitochondria
Weather
Fog
Reactive Oxygen Species
Oxygen
90 Citations (Scopus)

Regulation of hypoxia-induced pulmonary hypertension by vascular smooth muscle hypoxia-inducible factor-1α

Ball, M. K., Waypa, G. B., Mungai, P. T., Nielsen, J. M., Czech, L., Dudley, V. J., Beussink, L., Dettman, R., Berkelhamer, S. K., Steinhorn, R. H., Shah, S. J. & Schumacker, P. T., Feb 1 2014, In : American Journal of Respiratory and Critical Care Medicine. 189, 3, p. 314-324 11 p.

Research output: Contribution to journalArticle

Hypoxia-Inducible Factor 1
Vascular Smooth Muscle
Pulmonary Hypertension
Smooth Muscle
Tamoxifen