Grants per year
Personal profile
Research Interests
We are interested in the basic mechanisms of cell cycle control, cellular senescence/immortalization and malignant transformation, with a focus on protein regulation by ubiquitination. We previously demonstrated that cell cycle regulators such as p27Kip1, CDK4 and CDC25A play highly tissue-specific roles in development and oncogenesis. Ubiquitination, the covalent modification of substrate proteins with the small 76-residue protein ubiquitin, exerts diverse regulation of the fate of substrates, including the cell cycle regulators, e.g, promoting proteolysis, altering subcellular localization and modulating enzymatic activities. Our current research is aimed at revealing novel functions of ubiquitination enzymes and their substrates in development and cancer, which is expected to identify new therapeutic targets against human diseases. The laboratory uses a combination of protein engineering, proteomics, bioinformatics, cell biological techniques such as time-lapse microscopy and 3-D culture, and genetically engineered mouse models. Keywords: cell cycle, ubiquitin, ubiquitination, cancer initiation, cancer progression, knockout mice, transgenic mice, breast cancer, cyclin, diabetes, pituitary, development.
Training Experience
1994 | Postdoctoral Fellowship, Memorial Sloan-Kettering Cancer Center |
1995 | Postdoctoral Fellowship, Memorial Sloan-Kettering Cancer Center |
Education/Academic qualification
PhD, Osaka University
… → 1990
MD, Osaka University
… → 1986
Research interests
- Biochemistry: Proteins
- Bioinformatics
- Breast Cancer
- Cancer Biology
- Endocrinology
- Molecular Biology
- Proteomics
- Transgenics
Fingerprint
- 1 Similar Profiles
Network
Grants
-
Orthogonal Ubiquitin Transfer to Profile E3 Substrate Specificity
Georgia State University, National Institute of General Medical Sciences
7/1/19 → 6/30/21
Project: Research project
-
Orthogonal Ubiquitin Transfer to Profile E3 Substrate Specificity
Georgia State University, National Institute of General Medical Sciences
9/1/18 → 6/30/22
Project: Research project
-
RHLCCC: Program Leaders
Backman, V., Benson III, A. B., Carthew, R. W., Chakravarti, D., Cheng, C., Eklund, E. A., Gius, D. R., Gordon, L. I., Green, K. J., Horvath, C. M., Hou, L., Khan, S. A., Kiyokawa, H., LaBonne, C., Penedo, F. J., Platanias, L. C., Simon, M. A., Spring, B. & Woloschak, G. E.
8/1/16 → 7/31/18
Project: Research project
-
Training Program in Oncogenesis and Developmental Biology
Eklund, E. A., Kiyokawa, H., LaBonne, C. & Leikin, R. G.
9/24/14 → 10/31/19
Project: Research project
-
Targeting a Novel Signaling Network Against Lung Metastasis of Breast Cancer
Northwestern Memorial Hospital
9/1/14 → 8/31/16
Project: Research project
Research Output
-
Erratum: Cdk2 and Cdk4 regulate the centrosome cycle and are critical mediators of centrosome amplification in p53-null cells (Molecular and Cellular Biology (2010) 30:3 (694-710) DOI: 10.1128/MCB.00253-09)
Adon, A. M., Zeng, X., Harrison, M. K., Sannem, S., Kiyokawa, H., Kaldis, P. & Saavedra, H. I., Dec 2020, In: Molecular and cellular biology. 40, 23, e00488.Research output: Contribution to journal › Comment/debate › peer-review
Open Access -
The HECT E3 ligase E6AP/UBE3A as a therapeutic target in cancer and neurological disorders
Owais, A., Mishra, R. K. & Kiyokawa, H., Aug 2020, In: Cancers. 12, 8, p. 1-19 19 p., 2108.Research output: Contribution to journal › Review article › peer-review
Open Access -
Identifying the substrate proteins of U-box E3s E4B and CHIP by orthogonal ubiquitin transfer
Bhuripanyo, K., Wang, Y., Liu, X., Zhou, L., Liu, R., Duong, D., Zhao, B., Bi, Y., Zhou, H., Chen, G., Seyfried, N. T., Chazin, W. J., Kiyokawa, H. & Yin, J., Jan 2018, In: Science Advances. 4, 1, e1701393.Research output: Contribution to journal › Article › peer-review
7 Scopus citations -
The balance of PI3K and ERK signaling is dysregulated in prolactinoma and modulated by dopamine
Roof, A. K., Jirawatnotai, S., Trudeau, T., Kuzyk, C., Wierman, M. E., Kiyokawa, H. & Gutierrez-Hartmann, A., Jun 1 2018, In: Endocrinology. 159, 6, p. 2421-2434 14 p.Research output: Contribution to journal › Article › peer-review
6 Scopus citations -
Identifying the ubiquitination targets of E6AP by orthogonal ubiquitin transfer
Wang, Y., Liu, X., Zhou, L., Duong, D., Bhuripanyo, K., Zhao, B., Zhou, H., Liu, R., Bi, Y., Kiyokawa, H. & Yin, J., Dec 1 2017, In: Nature communications. 8, 1, 2232.Research output: Contribution to journal › Article › peer-review
8 Scopus citations