• 7151 Citations
1987 …2019

Research output per year

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Personal profile

Research Interests

We are interested in the basic mechanisms of cell cycle control, cellular senescence/immortalization and malignant transformation, with a focus on protein regulation by ubiquitination. We previously demonstrated that cell cycle regulators such as p27Kip1, CDK4 and CDC25A play highly tissue-specific roles in development and oncogenesis. Ubiquitination, the covalent modification of substrate proteins with the small 76-residue protein ubiquitin, exerts diverse regulation of the fate of substrates, including the cell cycle regulators, e.g, promoting proteolysis, altering subcellular localization and modulating enzymatic activities. Our current research is aimed at revealing novel functions of ubiquitination enzymes and their substrates in development and cancer, which is expected to identify new therapeutic targets against human diseases. The laboratory uses a combination of protein engineering, proteomics, bioinformatics, cell biological techniques such as time-lapse microscopy and 3-D culture, and genetically engineered mouse models. Keywords: cell cycle, ubiquitin, ubiquitination, cancer initiation, cancer progression, knockout mice, transgenic mice, breast cancer, cyclin, diabetes, pituitary, development.

Training Experience

1994Postdoctoral Fellowship, Memorial Sloan-Kettering Cancer Center
1995Postdoctoral Fellowship, Memorial Sloan-Kettering Cancer Center

Education/Academic qualification

PhD, Osaka University

… → 1990

MD, Osaka University

… → 1986

Research interests

  • Biochemistry: Proteins
  • Bioinformatics
  • Breast Cancer
  • Cancer Biology
  • Endocrinology
  • Molecular Biology
  • Proteomics
  • Transgenics

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  • Research Output

    • 7151 Citations
    • 100 Article
    • 6 Review article
    • 1 Chapter
    • 1 Conference contribution

    Identifying the substrate proteins of U-box E3s E4B and CHIP by orthogonal ubiquitin transfer

    Bhuripanyo, K., Wang, Y., Liu, X., Zhou, L., Liu, R., Duong, D., Zhao, B., Bi, Y., Zhou, H., Chen, G., Seyfried, N. T., Chazin, W. J., Kiyokawa, H. & Yin, J., Jan 1 2018, In : Science Advances. 4, 1, e1701393.

    Research output: Contribution to journalArticle

  • 5 Scopus citations

    The balance of PI3K and ERK signaling is dysregulated in prolactinoma and modulated by dopamine

    Roof, A. K., Jirawatnotai, S., Trudeau, T., Kuzyk, C., Wierman, M. E., Kiyokawa, H. & Gutierrez-Hartmann, A., Jun 1 2018, In : Endocrinology. 159, 6, p. 2421-2434 14 p.

    Research output: Contribution to journalArticle

  • 5 Scopus citations

    Identifying the ubiquitination targets of E6AP by orthogonal ubiquitin transfer

    Wang, Y., Liu, X., Zhou, L., Duong, D., Bhuripanyo, K., Zhao, B., Zhou, H., Liu, R., Bi, Y., Kiyokawa, H. & Yin, J., Dec 1 2017, In : Nature communications. 8, 1, 2232.

    Research output: Contribution to journalArticle

  • 6 Scopus citations

    Orthogonal ubiquitin transfer identifies ubiquitination substrates under differential control by the two ubiquitin activating enzymes

    Liu, X., Zhao, B., Sun, L., Bhuripanyo, K., Wang, Y., Bi, Y., Davuluri, R. V., Duong, D. M., Nanavati, D., Yin, J. & Kiyokawa, H., Jan 30 2017, In : Nature communications. 8, 14286.

    Research output: Contribution to journalArticle

  • 19 Scopus citations

    Somatotroph-specific Aip-deficient mice display pretumorigenic alterations in cell-cycle signaling

    Gillam, M. P., Ku, C. R., Lee, Y. J., Kim, J., Kim, S. H., Lee, S. J., Hwang, B., Koo, J. H., Kineman, R. D., Kiyokawa, H. & Lee, E. J., Jan 1 2017, In : Journal of the Endocrine Society. 1, 2, p. 78-95 18 p.

    Research output: Contribution to journalArticle

    Open Access
  • 3 Scopus citations