• 7023 Citations
1987 …2022
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Personal profile

Research Interests

We are interested in the basic mechanisms of cell cycle control, cellular senescence/immortalization and malignant transformation, with a focus on protein regulation by ubiquitination. We previously demonstrated that cell cycle regulators such as p27Kip1, CDK4 and CDC25A play highly tissue-specific roles in development and oncogenesis. Ubiquitination, the covalent modification of substrate proteins with the small 76-residue protein ubiquitin, exerts diverse regulation of the fate of substrates, including the cell cycle regulators, e.g, promoting proteolysis, altering subcellular localization and modulating enzymatic activities. Our current research is aimed at revealing novel functions of ubiquitination enzymes and their substrates in development and cancer, which is expected to identify new therapeutic targets against human diseases. The laboratory uses a combination of protein engineering, proteomics, bioinformatics, cell biological techniques such as time-lapse microscopy and 3-D culture, and genetically engineered mouse models. Keywords: cell cycle, ubiquitin, ubiquitination, cancer initiation, cancer progression, knockout mice, transgenic mice, breast cancer, cyclin, diabetes, pituitary, development.

Training Experience

1994Postdoctoral Fellowship, Memorial Sloan-Kettering Cancer Center
1995Postdoctoral Fellowship, Memorial Sloan-Kettering Cancer Center

Education/Academic qualification

PhD, Osaka University

… → 1990

MD, Osaka University

… → 1986

Research interests

  • Biochemistry: Proteins
  • Bioinformatics
  • Breast Cancer
  • Cancer Biology
  • Endocrinology
  • Molecular Biology
  • Proteomics
  • Transgenics

Fingerprint Dive into the research topics where Hiroaki Kiyokawa is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

Cyclin-Dependent Kinases Medicine & Life Sciences
Cell Cycle Medicine & Life Sciences
Cyclin-Dependent Kinase 4 Medicine & Life Sciences
Ubiquitin Medicine & Life Sciences
Carcinogenesis Medicine & Life Sciences
Ubiquitination Medicine & Life Sciences
Leukemia, Erythroblastic, Acute Medicine & Life Sciences
Cell Proliferation Medicine & Life Sciences

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Grants 2005 2022

Substrate Specificity
Induced Pluripotent Stem Cells
Ubiquitin-Protein Ligases
Technology Transfer
Developmental Biology
Breast Neoplasms
Neoplasm Metastasis
Triple Negative Breast Neoplasms

Research Output 1987 2018

  • 7023 Citations
  • 99 Article
  • 6 Review article
  • 1 Chapter
  • 1 Conference contribution
4 Citations (Scopus)

Identifying the substrate proteins of U-box E3s E4B and CHIP by orthogonal ubiquitin transfer

Bhuripanyo, K., Wang, Y., Liu, X., Zhou, L., Liu, R., Duong, D., Zhao, B., Bi, Y., Zhou, H., Chen, G., Seyfried, N. T., Chazin, W. J., Kiyokawa, H. & Yin, J., Jan 1 2018, In : Science Advances. 4, 1, e1701393.

Research output: Contribution to journalArticle

HSC70 Heat-Shock Proteins
Cyclin-Dependent Kinase 4
Phosphoric Monoester Hydrolases
4 Citations (Scopus)

The balance of PI3K and ERK signaling is dysregulated in prolactinoma and modulated by dopamine

Roof, A. K., Jirawatnotai, S., Trudeau, T., Kuzyk, C., Wierman, M. E., Kiyokawa, H. & Gutierrez-Hartmann, A., Jun 1 2018, In : Endocrinology. 159, 6, p. 2421-2434 14 p.

Research output: Contribution to journalArticle

Phosphatidylinositol 3-Kinase
Dopamine Agonists
5 Citations (Scopus)

Identifying the ubiquitination targets of E6AP by orthogonal ubiquitin transfer

Wang, Y., Liu, X., Zhou, L., Duong, D., Bhuripanyo, K., Zhao, B., Zhou, H., Liu, R., Bi, Y., Kiyokawa, H. & Yin, J., Dec 1 2017, In : Nature communications. 8, 1, 2232.

Research output: Contribution to journalArticle

15 Citations (Scopus)

Orthogonal ubiquitin transfer identifies ubiquitination substrates under differential control by the two ubiquitin activating enzymes

Liu, X., Zhao, B., Sun, L., Bhuripanyo, K., Wang, Y., Bi, Y., Davuluri, R. V., Duong, D. M., Nanavati, D., Yin, J. & Kiyokawa, H., Jan 30 2017, In : Nature communications. 8, 14286.

Research output: Contribution to journalArticle

Ubiquitin-Activating Enzymes
Ubiquitin-Conjugating Enzymes
3 Citations (Scopus)

The non-canonical ubiquitin activating enzyme UBA6 suppresses epithelial-mesenchymal transition of mammary epithelial cells

Liu, X., Sun, L., Gursel, D. B., Cheng, C., Huang, S., Rademaker, A. W., Khan, S. A., Yin, J. & Kiyokawa, H., Jan 1 2017, In : Oncotarget. 8, 50, p. 87480-87493 14 p.

Research output: Contribution to journalArticle

Ubiquitin-Activating Enzymes
Epithelial-Mesenchymal Transition
Epithelial Cells