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Personal profile

Research Interests

The research in my lab is centered on the “espins,” a family of multifunctional actin-bundling proteins, and the elucidation of their roles in the organization and function of hair cell stereocilia. Espins are produced in different sized isoforms from a single gene. They are present in the parallel actin bundle of hair cell stereocilia and are the target of mutations that cause deafness and vestibular dysfunction in mice and humans. For example, the jerker mutation in the espin gene of mice causes deafness and vestibular dysfunction accompanied by abnormal stereocilia morphogenesis and accelerated stereocilia degeneration. In particular, the stereocilia of jerker homozygotes, which lack espin proteins, fail to increase in diameter during morphogenesis, suggesting that espins are required for the appositional growth of the stereocilia parallel actin bundle. Espins are also present in the microvilli of taste receptor cells, solitary chemoreceptor cells, vomeronasal sensory neurons and Merkel cells, raising the possibility that these proteins play general roles in the microvillar projections of vertebrate sensory cells. Unlike the mammalian plastin/fimbrin actin-bundling proteins found in stereocilia and microvilli, espins are not inhibited by calcium ion. In addition, espins are much more effective than fascin-1 at over-twisting actin filaments in parallel actin bundles and can do so even at low stoichiometry. In tranfected epithelial cells, espins dramatically elongate microvillar parallel actin bundles and could, therefore, also help determine the length of stereocilia and microvilli. Espins can bind actin monomer via their Wiskott-Aldrich Syndrome protein homology 2 (WH2) domain in vitro and in vivo and can assemble large actin bundles de novo when targeted to specific locations in transfected cells. These remarkable biological activities distinguish espins from other actin-bundling proteins and may make them especially well-suited to sensory cells.

Training Experience

1987Postdoctoral Fellowship, Johns Hopkins University

Education/Academic qualification

PhD, Washington University in St. Louis School of Medicine

… → 1981

Research interests

  • Cell Biology
  • Cytoskeleton
  • Genetics
  • Hearing
  • Molecular Biology
  • Neuroscience

Fingerprint Dive into the research topics where James Richard Bartles is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 2 Similar Profiles
Actins Medicine & Life Sciences
Stereocilia Medicine & Life Sciences
Cell Membrane Medicine & Life Sciences
Proteins Medicine & Life Sciences
Hepatocytes Medicine & Life Sciences
Microvilli Medicine & Life Sciences
Blood Proteins Medicine & Life Sciences
Membrane Proteins Medicine & Life Sciences

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Grants 2003 2019

Research Output 1979 2018

  • 2672 Citations
  • 53 Article
  • 4 Review article
  • 1 Comment/debate
2 Citations (Scopus)

Inframe deletion of human ESPN is associated with deafness, vestibulopathy and vision impairment

Ahmed, Z. M., Jaworek, T. J., Sarangdhar, G. N., Zheng, L., Gul, K., Khan, S. N., Friedman, T. B., Sisk, R. A., Bartles, J. R., Riazuddin, S. & Riazuddin, S., Jul 1 2018, In : Journal of medical genetics. 55, 7, p. 479-488 10 p.

Research output: Contribution to journalArticle

Usher Syndromes
Deafness
Exome
Actins
Organ of Corti
20 Citations (Scopus)

Fascin- and α-Actinin-Bundled Networks Contain Intrinsic Structural Features that Drive Protein Sorting

Winkelman, J. D., Suarez, C., Hocky, G. M., Harker, A. J., Morganthaler, A. N., Christensen, J. R., Voth, G. A., Bartles, J. R. & Kovar, D. R., Oct 24 2016, In : Current Biology. 26, 20, p. 2697-2706 10 p.

Research output: Contribution to journalArticle

microfilament proteins
Actinin
Microfilament Proteins
protein transport
Protein Transport
Stereocilia
Actins
Protein Isoforms
Proteins
14 Citations (Scopus)

Characterization and regulation of an additional actin-filamentbinding site in large isoforms of the stereocilia actin-bundling protein espin

Zheng, L., Beeler, D. M. & Bartles, J. R., Mar 1 2014, In : Journal of cell science. 127, 6, p. 1306-1317 12 p.

Research output: Contribution to journalArticle

Stereocilia
Actins
Myosin Type III
Protein Isoforms
Ankyrin Repeat
47 Citations (Scopus)
Stereocilia
Congenic Mice
morphogenesis
Morphogenesis
hair