• 12238 Citations
1984 …2020
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Personal profile

Research Interests

Our lab uses biophysical methods, with particular emphasis on micromanipulation of single DNA molecules and single chromosomes, to study the internal structure of chromosomes in vivo, and to study chromosome-organizing proteins and DNA topoisomerases in vitro. We also develop mathematical models relevant to these types of experiments. Projects in progress involve combining fluorescence microscopy and force microscopy in experiments on DNA-protein complexes and whole chromosomes, and in-vivo studies of coupling of chromosome dynamics to gene expression.

Education/Academic qualification

Physics, PhD, Massachusetts Institute of Technology

… → 1989

Physics, BSc, University of Alberta

… → 1984

Fingerprint Dive into the research topics where John F Marko is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 1 Similar Profiles
deoxyribonucleic acid Physics & Astronomy
DNA Medicine & Life Sciences
Chromosomes Medicine & Life Sciences
Chromatin Medicine & Life Sciences
helices Physics & Astronomy
Helix Mathematics
Micromanipulation Medicine & Life Sciences
Mechanics Medicine & Life Sciences

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Grants 2005 2020

Research Output 1984 2019

1 Citation (Scopus)

Chromatin's physical properties shape the nucleus and its functions

Stephens, A. D., Banigan, E. J. & Marko, J. F., Jun 1 2019, In : Current Opinion in Cell Biology. 58, p. 76-84 9 p.

Research output: Contribution to journalReview article

Chromatin
Lamins
Mechanics
Cytoskeleton
Cell Nucleus

DNA-segment-capture model for loop extrusion by structural maintenance of chromosome (SMC) protein complexes

Marko, J. F., De Los Rios, P., Barducci, A. & Gruber, S., Jul 26 2019, In : Nucleic acids research. 47, 13, p. 6956-6972 17 p.

Research output: Contribution to journalArticle

Open Access
Chromosomes
Maintenance
DNA
Proteins
Catalytic DNA
1 Citation (Scopus)

Effects of altering histone posttranslational modifications on mitotic chromosome structure and mechanics

Biggs, R., Liu, P. Z., Stephens, A. D. & Marko, J. F., Jan 1 2019, In : Molecular biology of the cell. 30, 7, p. 820-827 8 p.

Research output: Contribution to journalArticle

Histone Code
Chromosome Structures
Post Translational Protein Processing
Mechanics
Chromosomes

Mechanical unfolding of spectrin reveals a super-exponential dependence of unfolding rate on force

Renn, J. P., Bhattacharyya, S., Bai, H., He, C., Li, H., Oberhauser, A. F., Marko, J. F., Makarov, D. E. & Matouschek, A., Jul 31 2019, In : Scientific reports. 9, 1, 1 p.

Research output: Contribution to journalArticle

Open Access
Spectrin
Protein Unfolding
Atomic Force Microscopy
Proteins

Multimodal interference-based imaging of nanoscale structure and macromolecular motion uncovers UV induced cellular paroxysm

Gladstein, S., Almassalha, L. M., Cherkezyan, L., Chandler, J. E., Eshein, A., Eid, A., Zhang, D., Wu, W., Bauer, G. M., Stephens, A. D., Morochnik, S., Subramanian, H., Marko, J. F., Ameer, G. A., Szleifer, I. G. & Backman, V., Dec 1 2019, In : Nature communications. 10, 1, 1652.

Research output: Contribution to journalArticle

Open Access
interference
Imaging techniques
Light interference
Photobleaching
Cell death