Grants per year
Personal profile
Research Interests
Autopsy pathology, Cardiac pathology
Lipids are important regulators of the activity of many proteins including those involved in cardiac, vascular, pulmonary, and neural regulation, yet little is known about the molecular mechanisms mediating these effects. My laboratory staff members are engaged in a program of basic research to elucidate the molecular mechanisms by which lipids act as specific ligands to regulate cellular responses, and to investigate in select areas of cardiovascular disease how aberrations of signaling pathways may play a vital role in the pathogenesis of human disease.
We have used the family of phosphoinositide-specific phospholipase C isoforms as a model to study protein-lipid interactions. Upon stimulation by various hormones these membrane associated enzymes hydrolyze polyphosphoinositides to yield second messengers such diacylglycerol and inositol 1,4,5-trisphosphate. The determinants of enzyme function are complex and can be broken down into several primary components: substrate binding and catalysis, enzyme translocation, and regulation. During the past several years we have identified the structural motifs in the PLC delta 1 isoform that mediate each of the three primary functions. All three motifs require the binding of a specific lipid for function. The structural motif mediating translocation also modulates the rate catalysis, and is encoded by a unique domain termed the pleckstrin homology or PH domain. This newly discovered protein module of 100 amino acids exists in many molecules (including PLC d1) that participate in signal transduction. Our research is among the first to demonstrate a clear function in signal transduction for the PH domain, and serves a paradigm for the activation and regulation of many signaling molecules.
Certifications and Licenses
Anatomic Pathology |
Training Experience
1991 | Fellowship, Duke University Medical Center |
1992 | Residency, Duke University Medical Center |
Education/Academic qualification
MD, Dartmouth Medical School
… → 1987
Research interests
- Biochemistry: Lipids
- Cardiovascular Diseases
- Molecular Pharmacology
- Signal Transduction
- Vascular Biology
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Grants
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The identification and pathophysiology of non-infarcted but injured myocardium in the post-ischemic heart
Arora, R. K., Jin, J., Kume, T., Kwasny, M. J., Zhao, M. & Lomasney, J. W.
National Heart, Lung, and Blood Institute
1/1/21 → 12/31/24
Project: Research project
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Pathophysiological Significance of Atrial Fibrillation Electrogram Patterns
Arora, R. K., Ciolino, J. D., Ng, J., Arora, R. K. & Lomasney, J. W.
National Heart, Lung, and Blood Institute
8/1/15 → 7/31/21
Project: Research project
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The size of myocardial injury as a new criterion for assessing the pathological state of the heart
Arora, R. K., Zhao, M. & Lomasney, J. W.
American Heart Association Midwest Affiliate
7/1/16 → 6/30/18
Project: Research project
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Comprehensive Cardiac Structure-Function Analysis in Heart Transplantation
Benzuly, K. H., Carr, J., Carroll, T. J., Hazen, G. B., Lee, D. C., Lin, K., Markl, M., Ragin, A. B., Rigolin, V. H., Yancy, C. W. & Lomasney, J. W.
National Heart, Lung, and Blood Institute
2/4/14 → 1/31/20
Project: Research project
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Mechanisms of Erythrocytic Infection and Anemia in Malaria: Project 1
National Heart, Lung, and Blood Institute
9/1/05 → 8/31/10
Project: Research project
Research Output
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Lung transplantation for patients with severe COVID-19
Bharat, A., Querrey, M., Markov, N. S., Kim, S., Kurihara, C., Garza-Castillon, R., Manerikar, A., Shilatifard, A., Tomic, R., Politanska, Y., Abdala-Valencia, H., Yeldandi, A. V., Lomasney, J. W., Misharin, A. V. & Budinger, G. R. S., Dec 16 2020, In: Science translational medicine. 12, 574, eabe4282.Research output: Contribution to journal › Article › peer-review
Open Access -
Retraction: Withdrawal: High glucose stimulates synthesis of fibronectin via a novel protein kinase C, Rap1b, and B-Raf signaling pathway (The Journal of biological chemistry (2002) 277 44 (41725-41735))
Lin, S., Sahai, A., Chugh, S. S., Pan, X., Wallner, E. I., Danesh, F. R., Lomasney, J. W. & Kanwar, Y. S., Jun 28 2019, In: The Journal of biological chemistry. 294, 26, p. 10381 1 p.Research output: Contribution to journal › Comment/debate › peer-review
Open Access -
Constitutive expression of a dominant-negative TGF-β type II receptor in the posterior left atrium leads to beneficial remodeling of atrial fibrillation substrate
Kunamalla, A., Ng, J., Parini, V., Yoo, S., Mcgee, K. A., Tomson, T. T., Gordon, D., Thorp, E. B., Lomasney, J., Zhang, Q., Shah, S., Browne, S., Knight, B. P., Passman, R., Goldberger, J. J., Aistrup, G. & Arora, R., Jun 24 2016, In: Circulation research. 119, 1, p. 69-82 14 p.Research output: Contribution to journal › Article › peer-review
20 Scopus citations -
Fulminant capillary leak syndrome in a patient with systemic sclerosis treated with imatinib mesylate
Hinchcliff, M. E., Lomasney, J., Johnson, J. A. & Varga, J., Oct 1 2016, In: Rheumatology (United Kingdom). 55, 10, p. 1916-1918 3 p.Research output: Contribution to journal › Letter › peer-review
3 Scopus citations -
Reproducibility and observer variability of tissue phase mapping for the quantification of regional myocardial velocities
Lin, K., Chowdhary, V., Benzuly, K. H., Yancy, C. W., Lomasney, J. W., Rigolin, V. H., Anderson, A. S., Wilcox, J., Carr, J. & Markl, M., Aug 1 2016, In: International Journal of Cardiovascular Imaging. 32, 8, p. 1227-1234 8 p.Research output: Contribution to journal › Article › peer-review
13 Scopus citations