Grants per year
Personal profile
Research Interests
My research group works at the interface of systems biology and synthetic biology in order to probe and program the function of complex, multicellular systems to develop transformative biotechnologies and enable a new paradigm of design-driven medicine. Using the tools of synthetic biology, biomolecular engineering, computational systems biology, and gene therapy, we develop technologies including programmable cell-based “devices,” immune therapies for cancer and chronic disease, smart vaccines, biosensors for global health applications, and tools for advanced metabolic engineering. By bringing an engineering approach to the investigation, design, and construction of biological systems, the Leonard group is advancing the frontiers of design-driven medicine to address unmet medical needs and create safe, effective, and long-lasting treatment options that improve both quantity and quality of life.
Education/Academic qualification
Chemical Engineering, PhD, University of California, Berkeley
… → 2006
Chemical Engineering, BS, Stanford University
… → 2000
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Cell- and Tissue-Based Therapy
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Double-Stranded RNA
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RNA Interference
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Systems Biology
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Network
Recent external collaboration on country level. Dive into details by clicking on the dots.
Grants 2009 2022
RoL: EAGER: DESYN-C3 Moving information across synthetic membranes via engineered sensors
12/1/18 → 11/30/20
Project: Research project
Membranes
Sensors
Chemical reactions
Cells
Catalysts
Design-driven engineering of robust mammalian sense-and-respond functions
National Institute of Biomedical Imaging and Bioengineering
8/1/18 → 4/30/22
Project: Research project
Cell- and Tissue-Based Therapy
Synthetic Biology
Cell Engineering
Regenerative Medicine
Therapeutics
Elucidating a novel regulator of inflammation: the TLR4-Inhibitory Complex (TIC)
National Institute of Allergy and Infectious Diseases
12/22/17 → 11/30/19
Project: Research project
Toll-Like Receptor 4
Inflammation
Ligands
Endotoxins
Clustered Regularly Interspaced Short Palindromic Repeats
Collaborative Research: EAGER: Customized cell biosensors for interrogating cancer cell physiology
9/1/17 → 8/31/19
Project: Research project
Cell Physiological Phenomena
Biosensing Techniques
Immunotherapy
Research
Neoplasms
C-083: Targeted Delivery of Biomolecules to the CNS via Extracellular vesicles
Searle Funds at The Chicago Community Trust
1/1/17 → 12/31/21
Project: Research project
Research Output 1999 2018
Development of novel metabolite-responsive transcription factors via transposon-mediated protein fusion
Younger, A. K. D., Su, P. Y., Shepard, A. J., Udani, S. V., Cybulski, T. R., Tyo, K. E. J. & Leonard, J. N., Feb 1 2018, In : Protein Engineering, Design and Selection. 31, 2, p. 55-63 9 p.Research output: Contribution to journal › Article
Transcription factors
Biosensing Techniques
Metabolites
Biosensors
Transcription Factors
Enrichment of extracellular vesicle subpopulations via affinity chromatography
Hung, M. E., Lenzini, S. B., Stranford, D. M. & Leonard, J. N., Jan 1 2018, Methods in Molecular Biology. Humana Press Inc, p. 109-124 16 p. (Methods in Molecular Biology; vol. 1740).Research output: Chapter in Book/Report/Conference proceeding › Chapter
Affinity Chromatography
Extracellular Vesicles
Centrifugation
Proteins
Population
Highly Stable, Ultrasmall Polymer-Grafted Nanobins (usPGNs) with Stimuli-Responsive Capability
Hong, B. J., Iscen, A., Chipre, A. J., Li, M. M., Lee, O. S., Leonard, J. N., Schatz, G. C. & Nguyen, S. T., Mar 1 2018, In : Journal of Physical Chemistry Letters. 9, 5, p. 1133-1139 7 p.Research output: Contribution to journal › Article
carbopol 940
Cholesterol
stimuli
cholesterol
acrylic acid
Synthetic biology: Reframing cell therapy for cancer
Dolberg, T. B., Donahue, P. S. & Leonard, J. N., Feb 14 2018, In : Nature Chemical Biology. 14, 3, p. 204-205 2 p.Research output: Contribution to journal › Comment/debate
Synthetic Biology
Cell- and Tissue-Based Therapy
Neoplasms
A Systematic Evaluation of Factors Affecting Extracellular Vesicle Uptake by Breast Cancer Cells
Stranford, D. M., Hung, M. E., Gargus, E. S., Shah, R. N. & Leonard, J. N., Nov 1 2017, In : Tissue Engineering - Part A. 23, 21-22, p. 1274-1282 9 p.Research output: Contribution to journal › Article
Cells
Biomolecules
Breast Neoplasms
Hexadimethrine Bromide
Display devices