• 2581 Citations
19992024

Research output per year

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Research Output

2020

Macrophages employ quorum licensing to regulate collective activation

Muldoon, J. J., Chuang, Y., Bagheri, N. & Leonard, J. N., Dec 1 2020, In : Nature communications. 11, 1, 878.

Research output: Contribution to journalArticle

Open Access

The COMET toolkit for composing customizable genetic programs in mammalian cells

Donahue, P. S., Draut, J. W., Muldoon, J. J., Edelstein, H. I., Bagheri, N. & Leonard, J. N., Dec 1 2020, In : Nature communications. 11, 1, 779.

Research output: Contribution to journalArticle

Open Access
1 Scopus citations
2018

Advances, challenges, and opportunities in extracellular RNA biology: insights from the NIH exRNA Strategic Workshop

Li, K., Rodosthenous, R. S., Kashanchi, F., Gingeras, T., Gould, S. J., Kuo, L. S., Kurre, P., Lee, H., Leonard, J. N., Liu, H., Lombo, T. B., Momma, S., Nolan, J. P., Ochocinska, M. J., Pegtel, D. M., Sadovsky, Y., Sánchez-Madrid, F., Valdes, K. M., Vickers, K. C., Weaver, A. M. & 5 others, Witwer, K. W., Zeng, Y., Das, S., Raffai, R. L. & Howcroft, T. K., Apr 5 2018, In : JCI Insight. 3, 7

Research output: Contribution to journalReview article

Open Access
17 Scopus citations

Development of novel metabolite-responsive transcription factors via transposon-mediated protein fusion

Younger, A. K. D., Su, P. Y., Shepard, A. J., Udani, S. V., Cybulski, T. R., Tyo, K. E. J. & Leonard, J. N., Feb 1 2018, In : Protein Engineering, Design and Selection. 31, 2, p. 55-63 9 p.

Research output: Contribution to journalArticle

9 Scopus citations

Enrichment of extracellular vesicle subpopulations via affinity chromatography

Hung, M. E., Lenzini, S. B., Stranford, D. M. & Leonard, J. N., Jan 1 2018, Methods in Molecular Biology. Humana Press Inc., p. 109-124 16 p. (Methods in Molecular Biology; vol. 1740).

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Highly Stable, Ultrasmall Polymer-Grafted Nanobins (usPGNs) with Stimuli-Responsive Capability

Hong, B. J., Iscen, A., Chipre, A. J., Li, M. M., Lee, O. S., Leonard, J. N., Schatz, G. C. & Nguyen, S. T., Mar 1 2018, In : Journal of Physical Chemistry Letters. 9, 5, p. 1133-1139 7 p.

Research output: Contribution to journalArticle

2 Scopus citations

Synthetic biology: Reframing cell therapy for cancer

Dolberg, T. B., Donahue, P. S. & Leonard, J. N., Feb 14 2018, In : Nature Chemical Biology. 14, 3, p. 204-205 2 p.

Research output: Contribution to journalComment/debate

2 Scopus citations
2017

A Systematic Evaluation of Factors Affecting Extracellular Vesicle Uptake by Breast Cancer Cells

Stranford, D. M., Hung, M. E., Gargus, E. S., Shah, R. N. & Leonard, J. N., Nov 2017, In : Tissue Engineering - Part A. 23, 21-22, p. 1274-1282 9 p.

Research output: Contribution to journalArticle

3 Scopus citations

Building with intent: Technologies and principles for engineering mammalian cell-based therapies to sense and respond

Muldoon, J. J., Donahue, P. S., Dolberg, T. B. & Leonard, J. N., Dec 2017, In : Current Opinion in Biomedical Engineering. 4, p. 127-133 7 p.

Research output: Contribution to journalReview article

3 Scopus citations

Delivery of Biomolecules via Extracellular Vesicles: A Budding Therapeutic Strategy

Stranford, D. M. & Leonard, J. N., Jan 1 2017, In : Advances in Genetics. 98, p. 155-175 21 p.

Research output: Contribution to journalArticle

5 Scopus citations

Engineering Modular Biosensors to Confer Metabolite-Responsive Regulation of Transcription

Younger, A. K. D., Dalvie, N. C., Rottinghaus, A. G. & Leonard, J. N., Feb 17 2017, In : ACS synthetic biology. 6, 2, p. 311-325 15 p.

Research output: Contribution to journalArticle

19 Scopus citations

Extended Concerted Rotation Technique Enhances the Sampling Efficiency of the Computational Peptide-Design Algorithm

Xiao, X., Wang, Y., Leonard, J. N. & Hall, C. K., Nov 14 2017, In : Journal of Chemical Theory and Computation. 13, 11, p. 5709-5720 12 p.

Research output: Contribution to journalArticle

2 Scopus citations

Multiplexing Engineered Receptors for Multiparametric Evaluation of Environmental Ligands

Hartfield, R. M., Schwarz, K. A., Muldoon, J. J., Bagheri, N. & Leonard, J. N., Nov 17 2017, In : ACS synthetic biology. 6, 11, p. 2042-2055 14 p.

Research output: Contribution to journalArticle

9 Scopus citations

Predicting the Dynamics and Heterogeneity of Genomic DNA Content within Bacterial Populations across Variable Growth Regimes

Du Lac, M., Scarpelli, A. H., Younger, A. K. D., Bates, D. G. & Leonard, J. N., Jul 21 2017, In : ACS synthetic biology. 6, 7, p. 1131-1139 9 p.

Research output: Contribution to journalArticle

Rewiring human cellular input-output using modular extracellular sensors

Schwarz, K. A., Daringer, N. M., Dolberg, T. B. & Leonard, J. N., Feb 1 2017, In : Nature Chemical Biology. 13, 2, p. 202-209 8 p.

Research output: Contribution to journalArticle

51 Scopus citations
2016

Adding energy minimization strategy to peptide-design algorithm enables better search for RNA-binding peptides: Redesigned λ N peptide binds boxB RNA

Xiao, X., Hung, M. E., Leonard, J. N. & Hall, C. K., Oct 15 2016, In : Journal of computational chemistry. 37, 27, p. 2423-2435 13 p.

Research output: Contribution to journalArticle

5 Scopus citations

A platform for actively loading cargo RNA to elucidate limiting steps in EV-mediated delivery

Hung, M. E. & Leonard, J. N., May 13 2016, In : Journal of Extracellular Vesicles. 5, 13 p., 31027.

Research output: Contribution to journalArticle

41 Scopus citations

Engineering cell-based therapies to interface robustly with host physiology

Schwarz, K. A. & Leonard, J. N., Oct 1 2016, In : Advanced Drug Delivery Reviews. 105, p. 55-65 11 p.

Research output: Contribution to journalReview article

8 Scopus citations

Regulation of the IL-10-driven macrophage phenotype under incoherent stimuli

Chuang, Y., Hung, M. E., Cangelose, B. K. & Leonard, J. N., Nov 1 2016, In : Innate Immunity. 22, 8, p. 647-657 11 p.

Research output: Contribution to journalArticle

10 Scopus citations

Transforming growth factor-beta 1 delivery from microporous scaffolds decreases inflammation post-implant and enhances function of transplanted islets

Liu, J. M. H., Zhang, J., Zhang, X., Hlavaty, K. A., Ricci, C. F., Leonard, J. N., Shea, L. D. & Gower, R. M., Feb 1 2016, In : Biomaterials. 80, p. 11-19 9 p.

Research output: Contribution to journalArticle

37 Scopus citations
2015

Contributions of unique intracellular domains to switchlike biosensing by Toll-like receptor 4

Daringer, N. M., Schwarz, K. A. & Leonard, J. N., Apr 3 2015, In : Journal of Biological Chemistry. 290, 14, p. 8764-8777 14 p.

Research output: Contribution to journalArticle

4 Scopus citations

Developing a strategy for constructing modular biosensors

Younger, A. K. D., Dalvie, N. C., Rottinghaus, A. G. & Leonard, J. N., Jan 1 2015, Materials Engineering and Sciences Division 2015 - Core Programming Area at the 2015 AIChE Annual Meeting. AIChE, p. 175-192 18 p. (Materials Engineering and Sciences Division 2015 - Core Programming Area at the 2015 AIChE Annual Meeting).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Developing a strategy for constructing modular biosensors

Younger, A. K. D., Dalvie, N. C., Rottinghaus, A. G. & Leonard, J. N., Jan 1 2015, Chemical Engineers in Medicine 2015 - Topical Conference at the 2015 AIChE Annual Meeting. AIChE, p. 33-50 18 p. (Chemical Engineers in Medicine 2015 - Topical Conference at the 2015 AIChE Annual Meeting).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Developing a strategy for constructing modular biosensors

Younger, A. K. D., Dalvie, N. C., Rottinghaus, A. G. & Leonard, J. N., Jan 1 2015, Liaison Functions 2015 - Core Programming Area at the 2015 AIChE Annual Meeting. AIChE, p. 105-122 18 p. (Liaison Functions 2015 - Core Programming Area at the 2015 AIChE Annual Meeting).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Developing a strategy for constructing modular biosensors

Younger, A. K. D., Dalvie, N. C., Rottinghaus, A. G. & Leonard, J. N., Jan 1 2015, Food, Pharmaceutical and Bioengineering Division 2015 - Core Programming Area at the 2015 AIChE Meeting. AIChE, p. 245-262 18 p. (Food, Pharmaceutical and Bioengineering Division 2015 - Core Programming Area at the 2015 AIChE Meeting; vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Developing a strategy for constructing modular biosensors

Younger, A. K. D., Dalvie, N. C., Rottinghaus, A. G. & Leonard, J. N., Jan 1 2015, Nanoscale Science and Engineering Forum 2015 - Core Programming Area at the 2015 AIChE Annual Meeting. AIChE, p. 85-102 18 p. (Nanoscale Science and Engineering Forum 2015 - Core Programming Area at the 2015 AIChE Annual Meeting).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Developing a strategy for constructing modular biosensors

Younger, A. K. D., Dalvie, N. C., Rottinghaus, A. G. & Leonard, J. N., Jan 1 2015, Separations Division 2015 - Core Programming Area at the 2015 AIChE Annual Meeting. AIChE, p. 205-222 18 p. (Separations Division 2015 - Core Programming Area at the 2015 AIChE Annual Meeting; vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Modelling population cell cycle heterogeneity to deconvolute population-level measurements

Du Lac, M., Scarpelli, A., Leonard, J. & Bates, D. G., Jan 1 2015, Synthetic Biology: Engineering, Evolution, and Design Conference 2015, SEED 2015. AIChE, Vol. 2. p. 979-985 7 p.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Regulation of Bacterial Gene Expression by Protease-Alleviated Spatial Sequestration (PASS)

Pitner, R. A., Scarpelli, A. H. & Leonard, J. N., Sep 18 2015, In : ACS synthetic biology. 4, 9, p. 966-974 9 p.

Research output: Contribution to journalArticle

3 Scopus citations

Spatial and Functional Heterogeneities Shape Collective Behavior of Tumor-Immune Networks

Wells, D. K., Chuang, Y., Knapp, L. M., Brockmann, D., Kath, W. L. & Leonard, J. N., Apr 1 2015, In : PLoS computational biology. 11, 4, e1004181.

Research output: Contribution to journalArticle

10 Scopus citations

Stabilization of exosome-targeting peptides via engineered glycosylation

Hung, M. E. & Leonard, J. N., Mar 27 2015, In : Journal of Biological Chemistry. 290, 13, p. 8166-8172 7 p.

Research output: Contribution to journalArticle

85 Scopus citations

Therapeutic applications of extracellular vesicles: Clinical promise and open questions

György, B., Hung, M. E., Breakefield, X. O. & Leonard, J. N., Jan 6 2015, In : Annual Review of Pharmacology and Toxicology. 55, p. 439-464 26 p.

Research output: Contribution to journalReview article

197 Scopus citations
2014

A systems biology approach to blood

Corey, S. J., Kimmel, M. & Leonard, J. N., Jan 1 2014, In : Advances in experimental medicine and biology. 844

Research output: Contribution to journalArticle

1 Scopus citations

Engineered cell-based therapies: A vanguard of design-driven medicine

Dudek, R. M., Chuang, Y. & Leonard, J. N., Jan 1 2014, In : Advances in experimental medicine and biology. 844, p. 369-391 23 p.

Research output: Contribution to journalArticle

2 Scopus citations

Lentivirus delivery of IL-10 to promote and sustain macrophage polarization towards an anti-inflammatory phenotype

Boehler, R. M., Kuo, R., Shin, S., Goodman, A. G., Pilecki, M. A., Leonard, J. N. & Shea, L. D., Jun 2014, In : Biotechnology and Bioengineering. 111, 6, p. 1210-1221 12 p.

Research output: Contribution to journalArticle

53 Scopus citations

Modular Extracellular sensor architecture for engineering mammalian cell-based devices

Daringer, N. M., Dudek, R. M., Schwarz, K. A. & Leonard, J. N., Dec 19 2014, In : ACS synthetic biology. 3, 12, p. 892-902 11 p.

Research output: Contribution to journalArticle

47 Scopus citations

Modulation of leukocyte infiltration and phenotype in microporous tissue engineering scaffolds via vector induced IL-10 expression

Gower, R. M., Boehler, R. M., Azarin, S. M., Ricci, C. F., Leonard, J. N. & Shea, L. D., Feb 1 2014, In : Biomaterials. 35, 6, p. 2024-2031 8 p.

Research output: Contribution to journalArticle

44 Scopus citations

Remote control of engineered cell-based therapies via chemically programmable particles

Schwarz, K. A., Dudek, R. M., Daringer, N. & Leonard, J. N., Jan 1 2014, Food, Pharmaceutical and Bioengineering Division 2014 - Core Programming Area at the 2014 AIChE Annual Meeting. AIChE, Vol. 1. 1 p.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Systems hematology: An introduction

Corey, S. J., Kimmel, M. & Leonard, J. N., Jan 1 2014, In : Advances in experimental medicine and biology. 844, p. 3-10 8 p.

Research output: Contribution to journalArticle

The Rise of Mammals

Leonard, J. N., Dec 19 2014, In : ACS synthetic biology. 3, 12, p. 878-879 2 p.

Research output: Contribution to journalReview article

2013

Disruption of tumor-associated immune dysfunction via coupled multicellular networks

Chuang, Y. & Leonard, J. N., Jan 1 2013, Food, Pharmaceutical and Bioengineering Division 2013 - Core Programming Area at the 2013 AIChE Annual Meeting: Global Challenges for Engineering a Sustainable Future. AIChE, 1 p. (Food, Pharmaceutical and Bioengineering Division 2013 - Core Programming Area at the 2013 AIChE Annual Meeting: Global Challenges for Engineering a Sustainable Future).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

FedExosomes: Engineering therapeutic biological nanoparticles that truly deliver

Marcus, M. E. & Leonard, J. N., May 7 2013, In : Pharmaceuticals. 6, 5, p. 659-680 22 p.

Research output: Contribution to journalReview article

104 Scopus citations
2011

TLR3-specific double-stranded RNA oligonucleotide adjuvants induce dendritic cell cross-presentation, CTL responses, and antiviral protection

Jelinek, I., Leonard, J. N., Price, G. E., Brown, K. N., Meyer-Manlapat, A., Goldsmith, P. K., Wang, Y., Venzon, D., Epstein, S. L. & Segal, D. M., Feb 15 2011, In : Journal of Immunology. 186, 4, p. 2422-2429 8 p.

Research output: Contribution to journalArticle

116 Scopus citations
2010

Biology by design: From top to bottom and back

Leonard, J. N., Fritz, B. R., Timmerman, L. E., Daringer, N. M. & Jewett, M. C., Dec 1 2010, In : Journal of Biomedicine and Biotechnology. 2010, 232016.

Research output: Contribution to journalReview article

17 Scopus citations

Staying on message: Design principles for controlling nonspecific responses to siRNA

Samuel-Abraham, S. & Leonard, J. N., Dec 1 2010, In : FEBS Journal. 277, 23, p. 4828-4836 9 p.

Research output: Contribution to journalReview article

34 Scopus citations
2009

Predicting toll-like receptor structures and characterizing ligand binding

Leonard, J. N., Bell, J. K. & Segal, D. M., Dec 1 2009, Toll-Like Receptors: Methods and Protocols. McCoy, C. & O'Neill, L. (eds.). p. 55-67 13 p. (Methods in Molecular Biology; vol. 517).

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations
2008

HIV Evades RNA Interference Directed at TAR by an Indirect Compensatory Mechanism

Leonard, J. N., Shah, P. S., Burnett, J. C. & Schaffer, D. V., Nov 13 2008, In : Cell Host and Microbe. 4, 5, p. 484-494 11 p.

Research output: Contribution to journalArticle

43 Scopus citations

Mitochondrial DNA damage triggers mitochondrial-superoxide generation and apoptosis

Ricci, C., Pastukh, V., Leonard, J., Turrens, J., Wilson, G., Schaffer, D. & Schaffer, S. W., Feb 1 2008, In : American Journal of Physiology - Cell Physiology. 294, 2

Research output: Contribution to journalArticle

98 Scopus citations

Structural basis of toll-like receptor 3 signaling with double-stranded RNA

Liu, L., Botos, I., Wang, Y., Leonard, J. N., Shiloach, J., Segal, D. M. & Davies, D. R., Apr 18 2008, In : Science. 320, 5874, p. 379-381 3 p.

Research output: Contribution to journalArticle

475 Scopus citations

The TLR3 signaling complex forms by cooperative receptor dimerization

Leonard, J. N., Ghirlando, R., Askins, J., Bell, J. K., Margulies, D. H., Davies, D. R. & Segal, D. M., Jan 8 2008, In : Proceedings of the National Academy of Sciences of the United States of America. 105, 1, p. 258-263 6 p.

Research output: Contribution to journalArticle

153 Scopus citations