Personal profile

Research Interests

Our research group is motivated by a desire to understand, harness, and expand the capabilities of biological systems for compelling applications in medicine, materials, and energy. Specifically, we focus on designing, constructing, and modifying biological systems involved in protein synthesis and metabolism to (i) understand why nature’s designs work the way they do and (ii) open the way to products that have been impractical, if not impossible, to produce by other means. An innovative feature of our research program is the use of cell-free systems. The foundational principle is that we can conduct precise, complex biomolecular transformations without using intact cells, which provides an unprecedented and otherwise unattainable freedom of design to modify and control biological systems. For example, cell-free systems avoid the need to balance the tug-of-war that exists between the cell’s physiological and evolutionary objectives and the engineer’s process objectives. We are also pioneering new directions to repurpose the translation apparatus for synthetic biology. The goal is to monitor, interrogate, and understand the process of translation, and with this knowledge diversify, evolve and repurpose the ribosome and its peripheral machinery into a re-engineered machine to generate non-natural polymers as new classes of sequence-defined, evolvable matter. 

Our research activities are structured into four thrust areas: (1) cell-free protein synthesis and orthogonal translation systems, (2) engineered ribosomes, (3) metabolic engineering, and (4) glycosylation. These research areas are connected by approach (e.g., cell-free systems) and their focus on biocatalytic systems (e.g., the translation apparatus). They advance new understanding of biological knowledge and develop enabling technologies for the production of therapeutics, biomaterials, and biochemicals. 

 

Education / Academic qualification

Chemical Engineering, PhD, Stanford University

… → 2005

Chemical Engineering, MS, Stanford University

… → 2001

Chemical Engineering, Bioengineering specialization (summa cum laude), BS, University of California, Los Angeles

… → 1999

Keywords

  • Artificial life
  • Biotechnology
  • Metabolic control
  • Transcription factor-metabolite interactions
  • Metabolism
  • Integrated systems biology
  • Cellular physiology
  • Protein synthesis
  • Metabolic engineering
  • Synthetic biology
  • Cell-freebiology
  • Cancer

Fingerprint Fingerprint is based on mining the text of the persons scientific documents to create an index of weighted terms, which defines the key subjects of each individual researcher.

  • 4 Similar Profiles
Synthetic Biology Medicine & Life Sciences
Proteins Medicine & Life Sciences
Metabolic Engineering Medicine & Life Sciences
Escherichia coli Chemical Compounds
Ribosomes Medicine & Life Sciences
Yeasts Medicine & Life Sciences
Cell-Free System Medicine & Life Sciences
Yeast Engineering & Materials Science

Network Recent external collaboration on country level. Dive into details by clicking on the dots.

Research Output 2002 2018

1 Citations

A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases

Schoborg, J. A., Hershewe, J. M., Stark, J. C., Kightlinger, W., Kath, J. E., Jaroentomeechai, T., Natarajan, A., DeLisa, M. P. & Jewett, M. C. Mar 1 2018 In : Biotechnology and Bioengineering. 115, 3, p. 739-750 12 p.

Research output: Contribution to journalArticle

Glycosylation
Proteins
Testing
Enzymes
Polysaccharides

Controlling cell-free metabolism through physiochemical perturbations

Karim, A. S., Heggestad, J. T., Crowe, S. A. & Jewett, M. C. Jan 1 2018 In : Metabolic Engineering. 45, p. 86-94 9 p.

Research output: Contribution to journalArticle

1-Butanol
Butenes
Metabolism
Biosynthesis
Proteins

Expanding the palette of Streptomyces-based cell-free protein synthesis systems with enhanced yields

Li, J., Wang, H. & Jewett, M. C. Feb 15 2018 In : Biochemical Engineering Journal. 130, p. 29-33 5 p.

Research output: Contribution to journalArticle

Streptomyces
Proteins
Biosynthesis
Protein Biosynthesis
Biological Products

Toward an orthogonal central dogma

Liu, C. C., Jewett, M. C., Chin, J. W. & Voigt, C. A. Jan 16 2018 In : Nature Chemical Biology. 14, 2, p. 103-106 4 p.

Research output: Contribution to journalComment/debate

A Pipeline for Studying and Engineering Single-Subunit Oligosaccharyltransferases

Jaroentomeechai, T., Zheng, X., Hershewe, J., Stark, J. C., Jewett, M. C. & DeLisa, M. P. Jan 1 2017 Methods in Enzymology. Academic Press Inc., Vol. 597, p. 55-81 27 p. (Methods in Enzymology; vol. 597)

Research output: Chapter in Book/Report/Conference proceedingChapter

Glycosylation
Pipelines
Asparagine
Bearings (structural)
Dissection