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Research Output

2020

A cell-free system for production of 2,3-butanediol is robust to growth-toxic compounds

Kay, J. E. & Jewett, M. C., Jun 2020, In : Metabolic Engineering Communications. 10, e00114.

Research output: Contribution to journalArticle

Open Access

Apparent size and morphology of bacterial microcompartments varies with technique

Kennedy, N. W., Hershewe, J. M., Nichols, T. M., Roth, E. W., Wilke, C. D., Mills, C. E., Jewett, M. C. & Tullman-Ercek, D., Jan 1 2020, In : PloS one. 15, 3, e0226395.

Research output: Contribution to journalArticle

Open Access

Cell-free gene expression: an expanded repertoire of applications

Silverman, A. D., Karim, A. S. & Jewett, M. C., Mar 1 2020, In : Nature Reviews Genetics. 21, 3, p. 151-170 20 p.

Research output: Contribution to journalReview article

6 Scopus citations

Design of a Transcriptional Biosensor for the Portable, On-Demand Detection of Cyanuric Acid

Liu, X., Silverman, A. D., Alam, K. K., Iverson, E., Lucks, J. B., Jewett, M. C. & Raman, S., Jan 1 2020, (Accepted/In press) In : ACS synthetic biology.

Research output: Contribution to journalArticle

High-Throughput Synthesis and Analysis of Intact Glycoproteins Using SAMDI-MS

Techner, J. M., Kightlinger, W., Lin, L., Hershewe, J., Ramesh, A., Delisa, M. P., Jewett, M. C. & Mrksich, M., Jan 21 2020, In : Analytical Chemistry. 92, 2, p. 1963-1971 9 p.

Research output: Contribution to journalArticle

1 Scopus citations

In vitro ribosome synthesis and evolution through ribosome display

Hammerling, M. J., Fritz, B. R., Yoesep, D. J., Kim, D. S., Carlson, E. D. & Jewett, M. C., Dec 1 2020, In : Nature communications. 11, 1, 1108.

Research output: Contribution to journalArticle

Open Access

Mutational characterization and mapping of the 70S ribosome active site

d'Aquino, A. E., Azim, T., Aleksashin, N. A., Hockenberry, A. J., Krüger, A. & Jewett, M. C., Mar 18 2020, In : Nucleic acids research. 48, 5, p. 2777-2789 13 p.

Research output: Contribution to journalArticle

Open Access
1 Scopus citations

Point-of-Use Detection of Environmental Fluoride via a Cell-Free Riboswitch-Based Biosensor

Thavarajah, W., Silverman, A. D., Verosloff, M. S., Kelley-Loughnane, N., Jewett, M. C. & Lucks, J. B., Jan 17 2020, In : ACS synthetic biology. 9, 1, p. 10-18 9 p.

Research output: Contribution to journalArticle

1 Scopus citations

Sequential Glycosylation of Proteins with Substrate-Specific N-Glycosyltransferases

Lin, L., Kightlinger, W., Prabhu, S. K., Hockenberry, A. J., Li, C., Wang, L. X., Jewett, M. C. & Mrksich, M., Feb 26 2020, In : ACS Central Science. 6, 2, p. 144-154 11 p.

Research output: Contribution to journalArticle

Open Access
2019

A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

Kightlinger, W., Duncker, K. E., Ramesh, A., Thames, A. H., Natarajan, A., Stark, J. C., Yang, A., Lin, L., Mrksich, M., DeLisa, M. P. & Jewett, M. C., Dec 1 2019, In : Nature communications. 10, 1, 5404.

Research output: Contribution to journalArticle

Open Access
4 Scopus citations

Assembly and functionality of the ribosome with tethered subunits

Aleksashin, N. A., Leppik, M., Hockenberry, A. J., Klepacki, D., Vázquez-Laslop, N., Jewett, M. C., Remme, J. & Mankin, A. S., Dec 1 2019, In : Nature communications. 10, 1, 930.

Research output: Contribution to journalArticle

Open Access
6 Scopus citations

BioBits Health: Classroom Activities Exploring Engineering, Biology, and Human Health with Fluorescent Readouts

Stark, J. C., Huang, A., Hsu, K. J., Dubner, R. S., Forbrook, J., Marshalla, S., Rodriguez, F., Washington, M., Rybnicky, G. A., Nguyen, P. Q., Hasselbacher, B., Jabri, R., Kamran, R., Koralewski, V., Wightkin, W., Martinez, T. & Jewett, M. C., May 17 2019, In : ACS synthetic biology. 8, 5, p. 1001-1009 9 p.

Research output: Contribution to journalArticle

4 Scopus citations

Cell-free biosynthesis of limonene using enzyme-enriched Escherichia coli lysates

Dudley, Q. M., Nash, C. J. & Jewett, M. C., May 21 2019, In : Synthetic Biology. 4, 1, ysz003.

Research output: Contribution to journalArticle

Open Access
7 Scopus citations

Computational design of three-dimensional RNA structure and function

Yesselman, J. D., Eiler, D., Carlson, E. D., Gotrik, M. R., d’Aquino, A. E., Ooms, A. N., Kladwang, W., Carlson, P. D., Shi, X., Costantino, D. A., Herschlag, D., Lucks, J. B., Jewett, M. C., Kieft, J. S. & Das, R., Sep 1 2019, In : Nature nanotechnology. 14, 9, p. 866-873 8 p.

Research output: Contribution to journalArticle

2 Scopus citations

Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry

Silverman, A. D., Kelley-Loughnane, N., Lucks, J. B. & Jewett, M. C., Feb 15 2019, In : ACS synthetic biology. 8, 2, p. 403-414 12 p.

Research output: Contribution to journalArticle

15 Scopus citations

Engineered ribosomes with tethered subunits for expanding biological function

Carlson, E. D., d’Aquino, A. E., Kim, D. S., Fulk, E. M., Hoang, K., Szal, T., Mankin, A. S. & Jewett, M. C., Dec 1 2019, In : Nature communications. 10, 1, 3920.

Research output: Contribution to journalArticle

Open Access
2 Scopus citations

Engineering Sensor Proteins

Merkx, M., Smith, B. & Jewett, M., Dec 27 2019, In : ACS Sensors. 4, 12, p. 3089-3091 3 p.

Research output: Contribution to journalReview article

Open Access

Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology

Levine, M. Z., Gregorio, N. E., Jewett, M. C., Watts, K. R. & Oza, J. P., Feb 25 2019, In : Journal of visualized experiments : JoVE. 144

Research output: Contribution to journalArticle

3 Scopus citations

Expanding the limits of the second genetic code with ribozymes

Lee, J., Schwieter, K. E., Watkins, A. M., Kim, D. S., Yu, H., Schwarz, K. J., Lim, J., Coronado, J., Byrom, M., Anslyn, E. V., Ellington, A. D., Moore, J. S. & Jewett, M. C., Dec 1 2019, In : Nature communications. 10, 1, 5097.

Research output: Contribution to journalArticle

Open Access
3 Scopus citations

High-throughput mapping of CoA metabolites by SAMDI-MS to optimize the cell-free biosynthesis of HMG-CoA

O’Kane, P. T., Dudley, Q. M., McMillan, A. K., Jewett, M. C. & Mrksich, M., Jun 5 2019, In : Science Advances. 5, 6, eaaw9180.

Research output: Contribution to journalArticle

Open Access
3 Scopus citations

Organism Engineering for the Bioproduction of the Triaminotrinitrobenzene (TATB) Precursor Phloroglucinol (PG)

Meyer, A., Saaem, I., Silverman, A., Varaljay, V. A., Mickol, R., Blum, S., Tobias, A. V., Schwalm, N. D., Mojadedi, W., Onderko, E., Bristol, C., Liu, S., Pratt, K., Casini, A., Eluere, R., Moser, F., Drake, C., Gupta, M., Kelley-Loughnane, N., Lucks, J. P. & 7 others, Akingbade, K. L., Lux, M. P., Glaven, S., Crookes-Goodson, W., Jewett, M. C., Gordon, D. B. & Voigt, C. A., Dec 20 2019, In : ACS synthetic biology. 8, 12, p. 2746-2755 10 p.

Research output: Contribution to journalArticle

Point-of-care biomarker quantification enabled by sample-specific calibration

McNerney, M. P., Zhang, Y., Steppe, P., Silverman, A. D., Jewett, M. C. & Styczynski, M. P., Sep 25 2019, In : Science Advances. 5, 9, eaax4473.

Research output: Contribution to journalArticle

Open Access
5 Scopus citations
2018

A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases

Schoborg, J. A., Hershewe, J. M., Stark, J. C., Kightlinger, W., Kath, J. E., Jaroentomeechai, T., Natarajan, A., DeLisa, M. P. & Jewett, M. C., Mar 2018, In : Biotechnology and Bioengineering. 115, 3, p. 739-750 12 p.

Research output: Contribution to journalArticle

20 Scopus citations

A novel framework for evaluating the performance of codon usage bias metrics

Liu, S. S., Hockenberry, A. J., Jewett, M. C. & Amaral, L. A. N., Jan 1 2018, In : Journal of the Royal Society Interface. 15, 138, 20170667.

Research output: Contribution to journalArticle

A Pressure Test to Make 10 Molecules in 90 Days: External Evaluation of Methods to Engineer Biology

Casini, A., Chang, F. Y., Eluere, R., King, A. M., Young, E. M., Dudley, Q. M., Karim, A., Pratt, K., Bristol, C., Forget, A., Ghodasara, A., Warden-Rothman, R., Gan, R., Cristofaro, A., Borujeni, A. E., Ryu, M. H., Li, J., Kwon, Y. C., Wang, H., Tatsis, E. & 7 others, Rodriguez-Lopez, C., O'Connor, S., Medema, M. H., Fischbach, M. A., Jewett, M. C., Voigt, C. & Gordon, D. B., Mar 28 2018, In : Journal of the American Chemical Society. 140, 12, p. 4302-4316 15 p.

Research output: Contribution to journalArticle

29 Scopus citations

BioBits™ Bright: A fluorescent synthetic biology education kit

Stark, J. C., Huang, A., Nguyen, P. Q., Dubner, R. S., Hsu, K. J., Ferrante, T. C., Anderson, M., Kanapskyte, A., Mucha, Q., Packett, J. S., Patel, P., Patel, R., Qaq, D., Zondor, T., Burke, J., Martinez, T., Miller-Berry, A., Puppala, A., Reichert, K., Schmid, M. & 20 others, Brand, L., Hill, L. R., Chellaswamy, J. F., Faheem, N., Fetherling, S., Gong, E., Gonzalzles, E. M., Granito, T., Koritsaris, J., Nguyen, B., Ottman, S., Palffy, C., Patel, A., Skweres, S., Slaton, A., Woods, T. R., Donghia, N., Pardee, K., Collins, J. J. & Jewett, M. C., Aug 1 2018, In : Science Advances. 4, 8, eaat5107.

Research output: Contribution to journalArticle

19 Scopus citations

Biobits™ explorer: A modular synthetic biology education kit

Huang, A., Nguyen, P. Q., Stark, J. C., Takahashi, M. K., Donghia, N., Ferrante, T., Dy, A. J., Hsu, K. J., Dubner, R. S., Pardee, K., Jewett, M. C. & Collins, J. J., Aug 1 2018, In : Science Advances. 4, 8, eaat5105.

Research output: Contribution to journalArticle

21 Scopus citations

Cell-free biomanufacturing

Bundy, B. C., Hunt, J. P., Jewett, M. C., Swartz, J. R., Wood, D. W., Frey, D. D. & Rao, G., Dec 2018, In : Current Opinion in Chemical Engineering. 22, p. 177-183 7 p.

Research output: Contribution to journalReview article

7 Scopus citations

Cell-free protein synthesis: An emerging technology for understanding, harnessing, and expanding the capabilities of biological systems

Schoborg, J. A. & Jewett, M. C., Mar 15 2018, Synthetic Biology: Parts, Devices and Applications. Wiley-VCH Verlag, p. 309-330 22 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids

Martin, R. W., Des Soye, B. J., Kwon, Y. C., Kay, J., Davis, R. G., Thomas, P. M., Majewska, N. I., Chen, C. X., Marcum, R. D., Weiss, M. G., Stoddart, A. E., Amiram, M., Ranji Charna, A. K., Patel, J. R., Isaacs, F. J., Kelleher, N. L., Hong, S. H. & Jewett, M. C., Dec 1 2018, In : Nature communications. 9, 1, 1203.

Research output: Contribution to journalArticle

39 Scopus citations

Cell-Free Synthetic Biology for Pathway Prototyping

Karim, A. S. & Jewett, M. C., Jan 1 2018, Methods in Enzymology. Scrutton, N. (ed.). Academic Press Inc, p. 31-57 27 p. (Methods in Enzymology; vol. 608).

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Controlling cell-free metabolism through physiochemical perturbations

Karim, A. S., Heggestad, J. T., Crowe, S. A. & Jewett, M. C., Jan 2018, In : Metabolic Engineering. 45, p. 86-94 9 p.

Research output: Contribution to journalArticle

21 Scopus citations

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases article

Kightlinger, W., Lin, L., Rosztoczy, M., Li, W., Delisa, M. P., Mrksich, M. & Jewett, M. C., Jun 1 2018, In : Nature Chemical Biology. 14, 6, p. 627-635 9 p.

Research output: Contribution to journalArticle

32 Scopus citations

Diversity of translation initiation mechanisms across bacterial species is driven by environmental conditions and growth demands

Hockenberry, A. J., Stern, A. J., Amaral, L. A. N. & Jewett, M. C., Jan 1 2018, In : Molecular biology and evolution. 35, 3, p. 582-592 11 p.

Research output: Contribution to journalArticle

4 Scopus citations

Engineered ribosomes for basic science and synthetic biology

D'Aquino, A. E., Kim, D. S. & Jewett, M. C., Jan 1 2018, In : Annual Review of Chemical and Biomolecular Engineering. 9, p. 311-340 30 p.

Research output: Contribution to journalReview article

13 Scopus citations

Erratum to: Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery (Nature Communications, (2018), 9, 1, (2686), 10.1038/s41467-018-05110-x)

Jaroentomeechai, T., Stark, J. C., Natarajan, A., Glasscock, C. J., Yates, L. E., Hsu, K. J., Mrksich, M., Jewett, M. C. & DeLisa, M. P., Dec 1 2018, In : Nature communications. 9, 1, 3396.

Research output: Contribution to journalComment/debate

1 Scopus citations

Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens

Des Soye, B. J., Davidson, S. R., Weinstock, M. T., Gibson, D. G. & Jewett, M. C., Sep 21 2018, In : ACS synthetic biology. 7, 9, p. 2245-2255 11 p.

Research output: Contribution to journalArticle

16 Scopus citations

Expanding the palette of Streptomyces-based cell-free protein synthesis systems with enhanced yields

Li, J., Wang, H. & Jewett, M. C., Feb 15 2018, In : Biochemical Engineering Journal. 130, p. 29-33 5 p.

Research output: Contribution to journalArticle

10 Scopus citations

High-Throughput Optimization Cycle of a Cell-Free Ribosome Assembly and Protein Synthesis System

Caschera, F., Karim, A. S., Gazzola, G., D'Aquino, A. E., Packard, N. H. & Jewett, M. C., Oct 24 2018, In : ACS synthetic biology. 7, 12, p. 2841-2853 13 p.

Research output: Contribution to journalArticle

4 Scopus citations

How many human proteoforms are there?

Aebersold, R., Agar, J. N., Amster, I. J., Baker, M. S., Bertozzi, C. R., Boja, E. S., Costello, C. E., Cravatt, B. F., Fenselau, C., Garcia, B. A., Ge, Y., Gunawardena, J., Hendrickson, R. C., Hergenrother, P. J., Huber, C. G., Ivanov, A. R., Jensen, O. N., Jewett, M. C., Kelleher, N. L., Kiessling, L. L. & 33 others, Krogan, N. J., Larsen, M. R., Loo, J. A., Ogorzalek Loo, R. R., Lundberg, E., Maccoss, M. J., Mallick, P., Mootha, V. K., Mrksich, M., Muir, T. W., Patrie, S. M., Pesavento, J. J., Pitteri, S. J., Rodriguez, H., Saghatelian, A., Sandoval, W., Schlüter, H., Sechi, S., Slavoff, S. A., Smith, L. M., Snyder, M. P., Thomas, P. M., Uhlén, M., Van Eyk, J. E., Vidal, M., Walt, D. R., White, F. M., Williams, E. R., Wohlschlager, T., Wysocki, V. H., Yates, N. A., Young, N. L. & Zhang, B., Feb 14 2018, In : Nature Chemical Biology. 14, 3, p. 206-214 9 p.

Research output: Contribution to journalReview article

100 Scopus citations

Incorporation of Nonproteinogenic Amino Acids in Class i and II Lantibiotics

Kakkar, N., Perez, J. G., Liu, W. R., Jewett, M. C. & Van Der Donk, W. A., Apr 20 2018, In : ACS chemical biology. 13, 4, p. 951-957 7 p.

Research output: Contribution to journalArticle

6 Scopus citations

Lost in translation: Mapping ribosomal active site mutations in vitro

D'Aquino, A., Azim, T., Hockenberry, A. J., Aleksashin, N., Mankin, A. & Jewett, M. C., Jan 1 2018, International Conference on Biomolecular Engineering, ICBE Asia 2018. AIChE, p. 5-6 2 p. (International Conference on Biomolecular Engineering, ICBE Asia 2018).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Neisseria gonorrhoeae Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response

Quillin, S. J., Hockenberry, A. J., Jewett, M. C. & Steven Seifert, H., Sep 1 2018, In : mSystems. 3, 5, e0015618.

Research output: Contribution to journalArticle

4 Scopus citations

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

Jaroentomeechai, T., Stark, J. C., Natarajan, A., Glasscock, C. J., Yates, L. E., Hsu, K. J., Mrksich, M., Jewett, M. C. & Delisa, M. P., Dec 1 2018, In : Nature communications. 9, 1, 2686.

Research output: Contribution to journalArticle

35 Scopus citations

Toward an orthogonal central dogma

Liu, C. C., Jewett, M. C., Chin, J. W. & Voigt, C. A., Jan 16 2018, In : Nature Chemical Biology. 14, 2, p. 103-106 4 p.

Research output: Contribution to journalComment/debate

31 Scopus citations

Within-gene shine-dalgarno sequences are not selected for function

Hockenberry, A. J., Jewett, M. C., Amaral, L. A. N. & Wilke, C. O., Jan 1 2018, In : Molecular biology and evolution. 35, 10, p. 2487-2498 12 p.

Research output: Contribution to journalArticle

1 Scopus citations
2017

A Pipeline for Studying and Engineering Single-Subunit Oligosaccharyltransferases

Jaroentomeechai, T., Zheng, X., Hershewe, J., Stark, J. C., Jewett, M. C. & DeLisa, M. P., Jan 1 2017, Methods in Enzymology. Academic Press Inc, p. 55-81 27 p. (Methods in Enzymology; vol. 597).

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Cell-Free Synthetic Systems for Metabolic Engineering and Biosynthetic Pathway Prototyping 125

Karim, A. S., Dudley, Q. M. & Jewett, M. C., 2017, Industrial Biotechnology: Microorganisms. Wittmann, C. & Liao, J. C. (eds.). Wiley Publishers, p. 125-148 24 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Cogenerating Synthetic Parts toward a Self-Replicating System

Li, J., Haas, W., Jackson, K., Kuru, E., Jewett, M. C., Fan, Z. H., Gygi, S. & Church, G. M., Jul 21 2017, In : ACS synthetic biology. 6, 7, p. 1327-1336 10 p.

Research output: Contribution to journalArticle

16 Scopus citations