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2020

A cell-free system for production of 2,3-butanediol is robust to growth-toxic compounds

Kay, J. E. & Jewett, M. C., Jun 2020, In : Metabolic Engineering Communications. 10, e00114.

Research output: Contribution to journalArticle

Open Access

Apparent size and morphology of bacterial microcompartments varies with technique

Kennedy, N. W., Hershewe, J. M., Nichols, T. M., Roth, E. W., Wilke, C. D., Mills, C. E., Jewett, M. C. & Tullman-Ercek, D., Jan 1 2020, In : PloS one. 15, 3, e0226395.

Research output: Contribution to journalArticle

Open Access

Design of a Transcriptional Biosensor for the Portable, On-Demand Detection of Cyanuric Acid

Liu, X., Silverman, A. D., Alam, K. K., Iverson, E., Lucks, J. B., Jewett, M. C. & Raman, S., Jan 1 2020, (Accepted/In press) In : ACS synthetic biology.

Research output: Contribution to journalArticle

High-Throughput Synthesis and Analysis of Intact Glycoproteins Using SAMDI-MS

Techner, J. M., Kightlinger, W., Lin, L., Hershewe, J., Ramesh, A., Delisa, M. P., Jewett, M. C. & Mrksich, M., Jan 21 2020, In : Analytical Chemistry. 92, 2, p. 1963-1971 9 p.

Research output: Contribution to journalArticle

1 Scopus citations

In vitro ribosome synthesis and evolution through ribosome display

Hammerling, M. J., Fritz, B. R., Yoesep, D. J., Kim, D. S., Carlson, E. D. & Jewett, M. C., Dec 1 2020, In : Nature communications. 11, 1, 1108.

Research output: Contribution to journalArticle

Open Access

Mutational characterization and mapping of the 70S ribosome active site

d'Aquino, A. E., Azim, T., Aleksashin, N. A., Hockenberry, A. J., Krüger, A. & Jewett, M. C., Mar 18 2020, In : Nucleic acids research. 48, 5, p. 2777-2789 13 p.

Research output: Contribution to journalArticle

Open Access
1 Scopus citations

Point-of-Use Detection of Environmental Fluoride via a Cell-Free Riboswitch-Based Biosensor

Thavarajah, W., Silverman, A. D., Verosloff, M. S., Kelley-Loughnane, N., Jewett, M. C. & Lucks, J. B., Jan 17 2020, In : ACS synthetic biology. 9, 1, p. 10-18 9 p.

Research output: Contribution to journalArticle

1 Scopus citations

Sequential Glycosylation of Proteins with Substrate-Specific N-Glycosyltransferases

Lin, L., Kightlinger, W., Prabhu, S. K., Hockenberry, A. J., Li, C., Wang, L. X., Jewett, M. C. & Mrksich, M., Feb 26 2020, In : ACS Central Science. 6, 2, p. 144-154 11 p.

Research output: Contribution to journalArticle

Open Access
2019

A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

Kightlinger, W., Duncker, K. E., Ramesh, A., Thames, A. H., Natarajan, A., Stark, J. C., Yang, A., Lin, L., Mrksich, M., DeLisa, M. P. & Jewett, M. C., Dec 1 2019, In : Nature communications. 10, 1, 5404.

Research output: Contribution to journalArticle

Open Access
4 Scopus citations

Assembly and functionality of the ribosome with tethered subunits

Aleksashin, N. A., Leppik, M., Hockenberry, A. J., Klepacki, D., Vázquez-Laslop, N., Jewett, M. C., Remme, J. & Mankin, A. S., Dec 1 2019, In : Nature communications. 10, 1, 930.

Research output: Contribution to journalArticle

Open Access
6 Scopus citations

BioBits Health: Classroom Activities Exploring Engineering, Biology, and Human Health with Fluorescent Readouts

Stark, J. C., Huang, A., Hsu, K. J., Dubner, R. S., Forbrook, J., Marshalla, S., Rodriguez, F., Washington, M., Rybnicky, G. A., Nguyen, P. Q., Hasselbacher, B., Jabri, R., Kamran, R., Koralewski, V., Wightkin, W., Martinez, T. & Jewett, M. C., May 17 2019, In : ACS synthetic biology. 8, 5, p. 1001-1009 9 p.

Research output: Contribution to journalArticle

4 Scopus citations

Cell-free biosynthesis of limonene using enzyme-enriched Escherichia coli lysates

Dudley, Q. M., Nash, C. J. & Jewett, M. C., May 21 2019, In : Synthetic Biology. 4, 1, ysz003.

Research output: Contribution to journalArticle

Open Access
7 Scopus citations

Computational design of three-dimensional RNA structure and function

Yesselman, J. D., Eiler, D., Carlson, E. D., Gotrik, M. R., d’Aquino, A. E., Ooms, A. N., Kladwang, W., Carlson, P. D., Shi, X., Costantino, D. A., Herschlag, D., Lucks, J. B., Jewett, M. C., Kieft, J. S. & Das, R., Sep 1 2019, In : Nature nanotechnology. 14, 9, p. 866-873 8 p.

Research output: Contribution to journalArticle

2 Scopus citations

Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry

Silverman, A. D., Kelley-Loughnane, N., Lucks, J. B. & Jewett, M. C., Feb 15 2019, In : ACS synthetic biology. 8, 2, p. 403-414 12 p.

Research output: Contribution to journalArticle

15 Scopus citations

Engineered ribosomes with tethered subunits for expanding biological function

Carlson, E. D., d’Aquino, A. E., Kim, D. S., Fulk, E. M., Hoang, K., Szal, T., Mankin, A. S. & Jewett, M. C., Dec 1 2019, In : Nature communications. 10, 1, 3920.

Research output: Contribution to journalArticle

Open Access
2 Scopus citations

Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology

Levine, M. Z., Gregorio, N. E., Jewett, M. C., Watts, K. R. & Oza, J. P., Feb 25 2019, In : Journal of visualized experiments : JoVE. 144

Research output: Contribution to journalArticle

3 Scopus citations

Expanding the limits of the second genetic code with ribozymes

Lee, J., Schwieter, K. E., Watkins, A. M., Kim, D. S., Yu, H., Schwarz, K. J., Lim, J., Coronado, J., Byrom, M., Anslyn, E. V., Ellington, A. D., Moore, J. S. & Jewett, M. C., Dec 1 2019, In : Nature communications. 10, 1, 5097.

Research output: Contribution to journalArticle

Open Access
3 Scopus citations

High-throughput mapping of CoA metabolites by SAMDI-MS to optimize the cell-free biosynthesis of HMG-CoA

O’Kane, P. T., Dudley, Q. M., McMillan, A. K., Jewett, M. C. & Mrksich, M., Jun 5 2019, In : Science Advances. 5, 6, eaaw9180.

Research output: Contribution to journalArticle

Open Access
3 Scopus citations

Organism Engineering for the Bioproduction of the Triaminotrinitrobenzene (TATB) Precursor Phloroglucinol (PG)

Meyer, A., Saaem, I., Silverman, A., Varaljay, V. A., Mickol, R., Blum, S., Tobias, A. V., Schwalm, N. D., Mojadedi, W., Onderko, E., Bristol, C., Liu, S., Pratt, K., Casini, A., Eluere, R., Moser, F., Drake, C., Gupta, M., Kelley-Loughnane, N., Lucks, J. P. & 7 others, Akingbade, K. L., Lux, M. P., Glaven, S., Crookes-Goodson, W., Jewett, M. C., Gordon, D. B. & Voigt, C. A., Dec 20 2019, In : ACS synthetic biology. 8, 12, p. 2746-2755 10 p.

Research output: Contribution to journalArticle

Point-of-care biomarker quantification enabled by sample-specific calibration

McNerney, M. P., Zhang, Y., Steppe, P., Silverman, A. D., Jewett, M. C. & Styczynski, M. P., Sep 25 2019, In : Science Advances. 5, 9, eaax4473.

Research output: Contribution to journalArticle

Open Access
5 Scopus citations
2018

A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases

Schoborg, J. A., Hershewe, J. M., Stark, J. C., Kightlinger, W., Kath, J. E., Jaroentomeechai, T., Natarajan, A., DeLisa, M. P. & Jewett, M. C., Mar 2018, In : Biotechnology and Bioengineering. 115, 3, p. 739-750 12 p.

Research output: Contribution to journalArticle

20 Scopus citations

A novel framework for evaluating the performance of codon usage bias metrics

Liu, S. S., Hockenberry, A. J., Jewett, M. C. & Amaral, L. A. N., Jan 1 2018, In : Journal of the Royal Society Interface. 15, 138, 20170667.

Research output: Contribution to journalArticle

A Pressure Test to Make 10 Molecules in 90 Days: External Evaluation of Methods to Engineer Biology

Casini, A., Chang, F. Y., Eluere, R., King, A. M., Young, E. M., Dudley, Q. M., Karim, A., Pratt, K., Bristol, C., Forget, A., Ghodasara, A., Warden-Rothman, R., Gan, R., Cristofaro, A., Borujeni, A. E., Ryu, M. H., Li, J., Kwon, Y. C., Wang, H., Tatsis, E. & 7 others, Rodriguez-Lopez, C., O'Connor, S., Medema, M. H., Fischbach, M. A., Jewett, M. C., Voigt, C. & Gordon, D. B., Mar 28 2018, In : Journal of the American Chemical Society. 140, 12, p. 4302-4316 15 p.

Research output: Contribution to journalArticle

29 Scopus citations

BioBits™ Bright: A fluorescent synthetic biology education kit

Stark, J. C., Huang, A., Nguyen, P. Q., Dubner, R. S., Hsu, K. J., Ferrante, T. C., Anderson, M., Kanapskyte, A., Mucha, Q., Packett, J. S., Patel, P., Patel, R., Qaq, D., Zondor, T., Burke, J., Martinez, T., Miller-Berry, A., Puppala, A., Reichert, K., Schmid, M. & 20 others, Brand, L., Hill, L. R., Chellaswamy, J. F., Faheem, N., Fetherling, S., Gong, E., Gonzalzles, E. M., Granito, T., Koritsaris, J., Nguyen, B., Ottman, S., Palffy, C., Patel, A., Skweres, S., Slaton, A., Woods, T. R., Donghia, N., Pardee, K., Collins, J. J. & Jewett, M. C., Aug 1 2018, In : Science Advances. 4, 8, eaat5107.

Research output: Contribution to journalArticle

19 Scopus citations

Biobits™ explorer: A modular synthetic biology education kit

Huang, A., Nguyen, P. Q., Stark, J. C., Takahashi, M. K., Donghia, N., Ferrante, T., Dy, A. J., Hsu, K. J., Dubner, R. S., Pardee, K., Jewett, M. C. & Collins, J. J., Aug 1 2018, In : Science Advances. 4, 8, eaat5105.

Research output: Contribution to journalArticle

21 Scopus citations

Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids

Martin, R. W., Des Soye, B. J., Kwon, Y. C., Kay, J., Davis, R. G., Thomas, P. M., Majewska, N. I., Chen, C. X., Marcum, R. D., Weiss, M. G., Stoddart, A. E., Amiram, M., Ranji Charna, A. K., Patel, J. R., Isaacs, F. J., Kelleher, N. L., Hong, S. H. & Jewett, M. C., Dec 1 2018, In : Nature communications. 9, 1, 1203.

Research output: Contribution to journalArticle

39 Scopus citations

Controlling cell-free metabolism through physiochemical perturbations

Karim, A. S., Heggestad, J. T., Crowe, S. A. & Jewett, M. C., Jan 2018, In : Metabolic Engineering. 45, p. 86-94 9 p.

Research output: Contribution to journalArticle

21 Scopus citations

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases article

Kightlinger, W., Lin, L., Rosztoczy, M., Li, W., Delisa, M. P., Mrksich, M. & Jewett, M. C., Jun 1 2018, In : Nature Chemical Biology. 14, 6, p. 627-635 9 p.

Research output: Contribution to journalArticle

32 Scopus citations

Diversity of translation initiation mechanisms across bacterial species is driven by environmental conditions and growth demands

Hockenberry, A. J., Stern, A. J., Amaral, L. A. N. & Jewett, M. C., Jan 1 2018, In : Molecular biology and evolution. 35, 3, p. 582-592 11 p.

Research output: Contribution to journalArticle

4 Scopus citations

Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens

Des Soye, B. J., Davidson, S. R., Weinstock, M. T., Gibson, D. G. & Jewett, M. C., Sep 21 2018, In : ACS synthetic biology. 7, 9, p. 2245-2255 11 p.

Research output: Contribution to journalArticle

16 Scopus citations

Expanding the palette of Streptomyces-based cell-free protein synthesis systems with enhanced yields

Li, J., Wang, H. & Jewett, M. C., Feb 15 2018, In : Biochemical Engineering Journal. 130, p. 29-33 5 p.

Research output: Contribution to journalArticle

10 Scopus citations

High-Throughput Optimization Cycle of a Cell-Free Ribosome Assembly and Protein Synthesis System

Caschera, F., Karim, A. S., Gazzola, G., D'Aquino, A. E., Packard, N. H. & Jewett, M. C., Oct 24 2018, In : ACS synthetic biology. 7, 12, p. 2841-2853 13 p.

Research output: Contribution to journalArticle

4 Scopus citations

Incorporation of Nonproteinogenic Amino Acids in Class i and II Lantibiotics

Kakkar, N., Perez, J. G., Liu, W. R., Jewett, M. C. & Van Der Donk, W. A., Apr 20 2018, In : ACS chemical biology. 13, 4, p. 951-957 7 p.

Research output: Contribution to journalArticle

6 Scopus citations

Neisseria gonorrhoeae Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response

Quillin, S. J., Hockenberry, A. J., Jewett, M. C. & Steven Seifert, H., Sep 1 2018, In : mSystems. 3, 5, e0015618.

Research output: Contribution to journalArticle

4 Scopus citations

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

Jaroentomeechai, T., Stark, J. C., Natarajan, A., Glasscock, C. J., Yates, L. E., Hsu, K. J., Mrksich, M., Jewett, M. C. & Delisa, M. P., Dec 1 2018, In : Nature communications. 9, 1, 2686.

Research output: Contribution to journalArticle

35 Scopus citations

Within-gene shine-dalgarno sequences are not selected for function

Hockenberry, A. J., Jewett, M. C., Amaral, L. A. N. & Wilke, C. O., Jan 1 2018, In : Molecular biology and evolution. 35, 10, p. 2487-2498 12 p.

Research output: Contribution to journalArticle

1 Scopus citations
2017

Cogenerating Synthetic Parts toward a Self-Replicating System

Li, J., Haas, W., Jackson, K., Kuru, E., Jewett, M. C., Fan, Z. H., Gygi, S. & Church, G. M., Jul 21 2017, In : ACS synthetic biology. 6, 7, p. 1327-1336 10 p.

Research output: Contribution to journalArticle

16 Scopus citations

Development of a CHO-Based Cell-Free Platform for Synthesis of Active Monoclonal Antibodies

Martin, R. W., Majewska, N. I., Chen, C. X., Albanetti, T. E., Jimenez, R. B. C., Schmelzer, A. E., Jewett, M. C. & Roy, V., Jul 21 2017, In : ACS synthetic biology. 6, 7, p. 1370-1379 10 p.

Research output: Contribution to journalArticle

30 Scopus citations

Establishing a high yielding streptomyces-based cell-free protein synthesis system

Li, J., Wang, H., Kwon, Y. C. & Jewett, M. C., Jun 2017, In : Biotechnology and Bioengineering. 114, 6, p. 1343-1353 11 p.

Research output: Contribution to journalArticle

23 Scopus citations
28 Scopus citations
10 Scopus citations

Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins

Gan, R., Perez, J. G., Carlson, E. D., Ntai, I., Isaacs, F. J., Kelleher, N. L. & Jewett, M. C., May 1 2017, In : Biotechnology and Bioengineering. 114, 5, p. 1074-1086 13 p.

Research output: Contribution to journalArticle

18 Scopus citations
2016

A cell-free expression and purification process for rapid production of protein biologics

Sullivan, C. J., Pendleton, E. D., Sasmor, H. H., Hicks, W. L., Farnum, J. B., Muto, M., Amendt, E. M., Schoborg, J. A., Martin, R. W., Clark, L. G., Anderson, M. J., Choudhury, A., Fior, R., Lo, Y. H., Griffey, R. H., Chappell, S. A., Jewett, M. C., Mauro, V. P. & Dresios, J., Feb 1 2016, In : Biotechnology Journal. 11, 2, p. 238-248 11 p.

Research output: Contribution to journalArticle

30 Scopus citations

A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery

Karim, A. S. & Jewett, M. C., Jul 1 2016, In : Metabolic Engineering. 36, p. 116-126 11 p.

Research output: Contribution to journalArticle

67 Scopus citations

Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with: In vitro synthesized and assembled ribosomes

Caschera, F., Lee, J. W., Ho, K. K. Y., Liu, A. P. & Jewett, M. C., Jan 1 2016, In : Chemical Communications. 52, 31, p. 5467-5469 3 p.

Research output: Contribution to journalArticle

26 Scopus citations

Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis

Dudley, Q. M., Anderson, K. C. & Jewett, M. C., Dec 16 2016, In : ACS synthetic biology. 5, 12, p. 1578-1588 11 p.

Research output: Contribution to journalArticle

48 Scopus citations

Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase

Li, J., Lawton, T. J., Kostecki, J. S., Nisthal, A., Fang, J., Mayo, S. L., Rosenzweig, A. C. & Jewett, M. C., Feb 1 2016, In : Biotechnology Journal. 11, 2, p. 212-218 7 p.

Research output: Contribution to journalArticle

20 Scopus citations

Cell-free synthetic biology: Engineering beyond the cell

Perez, J. G., Stark, J. C. & Jewett, M. C., Jan 1 2016, In : Cold Spring Harbor Perspectives in Biology. 8, 12, a023853.

Research output: Contribution to journalArticle

34 Scopus citations

Depletion of Shine-Dalgarno sequences within bacterial coding regions is expression dependent

Yang, C., Hockenberry, A. J., Jewett, M. C. & Amaralv, L. A. N., Jan 1 2016, In : G3: Genes, Genomes, Genetics. 6, 11, p. 3467-3474 8 p.

Research output: Contribution to journalArticle

8 Scopus citations