Grants per year
Personal profile
Research Interests
My group’s interests overlap chemistry, biology and engineering, with an emphasis on the design and synthesis of materials that are biologically active and in applications of the materials to relevant problems in the biological and medical sciences. Much of our work uses self-assembled monolayers of alkanethiolates on gold to prepare model surfaces that are structurally defined, yet that can have complex compositions and present the ligands in spatially-organized patterns. We pioneered the design of ‘dynamic substrates’ that present ligands whose activities can be switched on and off in response to electrical or optical signals, particularly for studies that address the responses of adherent cells to changes in the extracellular matrix. These mimics of the extracellular matrix have led the way to the discovery of novel ligands that mediate cell adhesion. We have also developed robust surface chemistries for preparing biochip arrays and that are compatible with new analytical methods for analyzing the arrays. For example, we have developed the SAMDI method, which uses mass spectrometry to analyze the arrays, and we have extended this method to the first label-free approach for high throughput screening, to the functional annotation of recently sequenced genes and towards an understanding of the networks that regulate protein acetylation. Finally, a recent program is creating defined systems for exploring biochemical reactions to understand the role that localization of enzymes and substrates play in controlling reaction networks.
Education/Academic qualification
Chemical Biology, PhD, Organic Chemistry, California Institute of Technology
… → 1994
Chemistry, BS, University of Illinois at Urbana-Champaign
… → 1989
Research interests
- Bio-inspired nanostructured materials and biochips
- Biomaterials and interfaces
- Cell adhesion and migration
- Electroactive interfaces
- Physical organic chemistry of interfacial reactions
Fingerprint
- 1 Similar Profiles
Network
-
Mrksich subproject - Cell-free Biomanufacturing Institute
U.S. Army Contracting Command - Rock Island
9/29/21 → 9/28/24
Project: Research project
-
Subproject for Dr. Mrksich
Defense Threat Reduction Agency
9/15/21 → 11/14/26
Project: Research project
-
Acquisition of a K2 Camera for Soft Matter Transmission Electron Microscopy
Gianneschi, N., Dichtel, W., Dravid, V. P., Farha, O. K., Joester, D., Meade, T. J., Mirkin, C. A., Mrksich, M., Scott, E. A., Stupp, S., Weiss, E. A. & Wu, J.
Office of the Director, National Institutes of Health
8/12/19 → 8/11/24
Project: Research project
-
Cell-free prototyping enables implementation of optimized reverse β-oxidation pathways in heterotrophic and autotrophic bacteria
Vögeli, B., Schulz, L., Garg, S., Tarasava, K., Clomburg, J. M., Lee, S. H., Gonnot, A., Moully, E. H., Kimmel, B. R., Tran, L., Zeleznik, H., Brown, S. D., Simpson, S. D., Mrksich, M., Karim, A. S., Gonzalez, R., Köpke, M. & Jewett, M. C., Dec 2022, In: Nature communications. 13, 1, 3058.Research output: Contribution to journal › Article › peer-review
Open Access4 Scopus citations -
Characterizing Enzyme Cooperativity with Imaging SAMDI-MS
Grant, J., Kimmel, B. R., Szymczak, L. C., Roll, J. & Mrksich, M., Feb 24 2022, In: Chemistry - A European Journal. 28, 12, e202103807.Research output: Contribution to journal › Article › peer-review
-
Efficient Enzymatic Incorporation of Dehydroalanine Based on SAMDI-Assisted Identification of Optimized Tags for OspF/SpvC
Yang, A., Tao, H., Szymczak, L. C., Lin, L., Song, J., Wang, Y., Bai, S., Modica, J., Huang, S. Y., Mrksich, M. & Feng, X., Feb 18 2022, In: ACS chemical biology. 17, 2, p. 414-425 12 p.Research output: Contribution to journal › Article › peer-review
-
High-Throughput Microfluidics Platform for Intracellular Delivery and Sampling of Biomolecules from Live Cells
Patino, C. A., Mukherjee, P., Berns, E. J., Moully, E. H., Stan, L., Mrksich, M. & Espinosa, H. D., 2022, (Accepted/In press) In: ACS nano.Research output: Contribution to journal › Article › peer-review
1 Scopus citations -
Scanning Transmission Electron Microscopy in a Scanning Electron Microscope for the High-Throughput Imaging of Biological Assemblies
Parker, K. A., Ribet, S., Kimmel, B. R., Dos Reis, R., Mrksich, M. & Dravid, V. P., Aug 8 2022, In: Biomacromolecules. 23, 8, p. 3235-3242 8 p.Research output: Contribution to journal › Article › peer-review
Datasets
-
CCDC 895622: Experimental Crystal Structure Determination
Cabrera-Pardo, J. R. (Creator), Chai, D. I. (Creator), Liu, S. (Creator), Mrksich, M. (Creator) & Kozmin, S. A. (Creator), Cambridge Crystallographic Data Centre, 2013
DOI: 10.5517/ccz1z10, http://www.ccdc.cam.ac.uk/services/structure_request?id=doi:10.5517/ccz1z10&sid=DataCite
Dataset
-
CCDC 895727: Experimental Crystal Structure Determination
Cabrera-Pardo, J. R. (Creator), Chai, D. I. (Creator), Liu, S. (Creator), Mrksich, M. (Creator) & Kozmin, S. A. (Creator), Cambridge Crystallographic Data Centre, 2013
DOI: 10.5517/ccz22fj, http://www.ccdc.cam.ac.uk/services/structure_request?id=doi:10.5517/ccz22fj&sid=DataCite
Dataset
-
CCDC 895621: Experimental Crystal Structure Determination
Cabrera-Pardo, J. R. (Creator), Chai, D. I. (Creator), Liu, S. (Creator), Mrksich, M. (Creator) & Kozmin, S. A. (Creator), Cambridge Crystallographic Data Centre, 2013
DOI: 10.5517/ccz1z0z, http://www.ccdc.cam.ac.uk/services/structure_request?id=doi:10.5517/ccz1z0z&sid=DataCite
Dataset
-
CCDC 895726: Experimental Crystal Structure Determination
Cabrera-Pardo, J. R. (Creator), Chai, D. I. (Creator), Liu, S. (Creator), Mrksich, M. (Creator) & Kozmin, S. A. (Creator), Cambridge Crystallographic Data Centre, 2013
DOI: 10.5517/ccz22dh, http://www.ccdc.cam.ac.uk/services/structure_request?id=doi:10.5517/ccz22dh&sid=DataCite
Dataset