If you made any changes in Pure these will be visible here soon.

Research Output

2020

A high-throughput SAMDI-mass spectrometry assay for isocitrate dehydrogenase 1

Anderson, S. E., Fahey, N. S., Park, J., O'Kane, P. T., Mirkin, C. A. & Mrksich, M., Jun 7 2020, In : The Analyst. 145, 11, p. 3899-3908 10 p.

Research output: Contribution to journalArticle

Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells

Schumann, B., Malaker, S. A., Wisnovsky, S. P., Debets, M. F., Agbay, A. J., Fernandez, D., Wagner, L. J. S., Lin, L., Li, Z., Choi, J., Fox, D. M., Peh, J., Gray, M. A., Pedram, K., Kohler, J. J., Mrksich, M. & Bertozzi, C. R., Jun 4 2020, In : Molecular cell. 78, 5, p. 824-834.e15

Research output: Contribution to journalArticle

Open Access
3 Scopus citations

Design and Synthesis of Megamolecule Mimics of a Therapeutic Antibody

Modica, J. A., Iderzorig, T. & Mrksich, M., Aug 12 2020, In : Journal of the American Chemical Society. 142, 32, p. 13657-13661 5 p.

Research output: Contribution to journalArticle

High-throughput photocapture approach for reaction discovery

Bayly, A. A., McDonald, B. R., Mrksich, M. & Scheidt, K. A., Jun 16 2020, In : Proceedings of the National Academy of Sciences of the United States of America. 117, 24, p. 13261-13266 6 p.

Research output: Contribution to journalArticle

3 Scopus citations

High-Throughput Synthesis and Analysis of Intact Glycoproteins Using SAMDI-MS

Techner, J. M., Kightlinger, W., Lin, L., Hershewe, J., Ramesh, A., Delisa, M. P., Jewett, M. C. & Mrksich, M., Jan 21 2020, In : Analytical Chemistry. 92, 2, p. 1963-1971 9 p.

Research output: Contribution to journalArticle

3 Scopus citations

Outer-Membrane Protease (OmpT) Based E. coli Sensing with Anionic Polythiophene and Unlabeled Peptide Substrate

Sinsinbar, G., Gudlur, S., Wood, S. E., Ammanath, G., Yildiz, H. U., Alagappan, P., Mrksich, M. & Liedberg, B., 2020, (Accepted/In press) In : Angewandte Chemie - International Edition.

Research output: Contribution to journalArticle

Outer-Membrane Protease (OmpT) Based E. coli Sensing with Anionic Polythiophene and Unlabeled Peptide Substrate

Sinsinbar, G., Gudlur, S., Wood, S. E., Ammanath, G., Yildiz, H. U., Alagappan, P., Mrksich, M. & Liedberg, B., 2020, (Accepted/In press) In : Angewandte Chemie.

Research output: Contribution to journalArticle

Role of lipopolysaccharide in protecting OmpT from autoproteolysis during in vitro refolding

Sinsinbar, G., Gudlur, S., Metcalf, K. J., Mrksich, M., Nallani, M. & Liedberg, B., Jun 2020, In : Biomolecules. 10, 6, p. 1-16 16 p., 922.

Research output: Contribution to journalArticle

Open Access

Sequential Glycosylation of Proteins with Substrate-Specific N-Glycosyltransferases

Lin, L., Kightlinger, W., Prabhu, S. K., Hockenberry, A. J., Li, C., Wang, L. X., Jewett, M. C. & Mrksich, M., Feb 26 2020, In : ACS Central Science. 6, 2, p. 144-154 11 p.

Research output: Contribution to journalArticle

Open Access
4 Scopus citations

Solid-Phase Synthesis of Megamolecules

Kimmel, B. R., Modica, J. A., Parker, K., Dravid, V. & Mrksich, M., Mar 11 2020, In : Journal of the American Chemical Society. 142, 10, p. 4534-4538 5 p.

Research output: Contribution to journalArticle

2 Scopus citations

Temporal Sampling of Enzymes from Live Cells by Localized Electroporation and Quantification of Activity by SAMDI Mass Spectrometry

Mukherjee, P., Berns, E. J., Patino, C. A., Hakim Moully, E., Chang, L., Nathamgari, S. S. P., Kessler, J. A., Mrksich, M. & Espinosa, H. D., Jul 1 2020, In : Small. 16, 26, 2000584.

Research output: Contribution to journalArticle

Using Peptide Arrays to Profile Phosphatase Activity in Cell Lysates

Szymczak, L. C., Sykora, D. J. & Mrksich, M., Jan 2 2020, In : Chemistry - A European Journal. 26, 1, p. 165-170 6 p.

Research output: Contribution to journalArticle

1 Scopus citations
2019

A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

Kightlinger, W., Duncker, K. E., Ramesh, A., Thames, A. H., Natarajan, A., Stark, J. C., Yang, A., Lin, L., Mrksich, M., DeLisa, M. P. & Jewett, M. C., Dec 1 2019, In : Nature communications. 10, 1, 5404.

Research output: Contribution to journalArticle

Open Access
18 Scopus citations

Exploration of the nanomedicine-design space with high-throughput screening and machine learning

Yamankurt, G., Berns, E. J., Xue, A., Lee, A., Bagheri, N., Mrksich, M. & Mirkin, C. A., Apr 1 2019, In : Nature Biomedical Engineering. 3, 4, p. 318-327 10 p.

Research output: Contribution to journalArticle

19 Scopus citations

High-throughput mapping of CoA metabolites by SAMDI-MS to optimize the cell-free biosynthesis of HMG-CoA

O’Kane, P. T., Dudley, Q. M., McMillan, A. K., Jewett, M. C. & Mrksich, M., Jun 5 2019, In : Science Advances. 5, 6, eaaw9180.

Research output: Contribution to journalArticle

Open Access
9 Scopus citations

Label-Free Assay of Protein Tyrosine Phosphatase Activity in Single Cells

Moully, E. H., Berns, E. J. & Mrksich, M., Oct 15 2019, In : Analytical Chemistry. 91, 20, p. 13206-13212 7 p.

Research output: Contribution to journalArticle

Plasmonic Microneedle Arrays for in Situ Sensing with Surface-Enhanced Raman Spectroscopy (SERS)

Park, J. E., Yonet-Tanyeri, N., Vander Ende, E., Henry, A. I., Perez White, B. E., Mrksich, M. & Van Duyne, R. P., Oct 9 2019, In : Nano letters. 19, 10, p. 6862-6868 7 p.

Research output: Contribution to journalArticle

7 Scopus citations

Sequential Photoactivation of Self-Assembled Monolayers to Direct Cell Adhesion and Migration

Bugga, P. & Mrksich, M., Apr 30 2019, In : Langmuir. 35, 17, p. 5937-5943 7 p.

Research output: Contribution to journalArticle

3 Scopus citations

Storage of Information Using Small Organic Molecules

Cafferty, B. J., Ten, A. S., Fink, M. J., Morey, S., Preston, D. J., Mrksich, M. & Whitesides, G. M., May 22 2019, In : ACS Central Science. 5, 5, p. 911-916 6 p.

Research output: Contribution to journalArticle

Open Access
22 Scopus citations

Subcellular Control over Focal Adhesion Anisotropy, Independent of Cell Morphology, Dictates Stem Cell Fate

Cabezas, M. D., Meckes, B., Mirkin, C. A. & Mrksich, M., Oct 22 2019, In : ACS nano. 13, 10, p. 11144-11152 9 p.

Research output: Contribution to journalArticle

3 Scopus citations

Using Microfluidics and Imaging SAMDI-MS to Characterize Reaction Kinetics

Grant, J., O'Kane, P. T., Kimmel, B. R. & Mrksich, M., Mar 27 2019, In : ACS Central Science. 5, 3, p. 486-493 8 p.

Research output: Contribution to journalArticle

Open Access

Using Peptide Arrays to Discover the Sequence-Specific Acetylation of the Histidine-Tyrosine Dyad

Szymczak, L. C. & Mrksich, M., Apr 2 2019, In : Biochemistry. 58, 13, p. 1810-1817 8 p.

Research output: Contribution to journalArticle

2018

An Immobilized Enzyme Reactor for Spatiotemporal Control over Reaction Products

Grant, J., Modica, J. A., Roll, J., Perkovich, P. & Mrksich, M., Aug 2 2018, In : Small. 14, 31, 1800923.

Research output: Contribution to journalArticle

5 Scopus citations

An Unusual Salt Effect in an Interfacial Nucleophilic Substitution Reaction

Li, S. & Mrksich, M., Jun 12 2018, In : Langmuir. 34, 23, p. 6713-6718 6 p.

Research output: Contribution to journalArticle

1 Scopus citations

Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase Activities

Szymczak, L. C., Huang, C. F., Berns, E. J. & Mrksich, M., Jan 1 2018, Methods in Enzymology. Allen, K. N. (ed.). Academic Press Inc, p. 389-403 15 p. (Methods in Enzymology; vol. 607).

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases article

Kightlinger, W., Lin, L., Rosztoczy, M., Li, W., Delisa, M. P., Mrksich, M. & Jewett, M. C., Jun 1 2018, In : Nature Chemical Biology. 14, 6, p. 627-635 9 p.

Research output: Contribution to journalArticle

38 Scopus citations

Dynamic substrates for cell biology

Bugga, P. & Mrksich, M., Nov 2018, In : Current Opinion in Colloid and Interface Science. 38, p. 80-87 8 p.

Research output: Contribution to journalReview article

5 Scopus citations

Efficient Syntheses of Diverse, Medicinally Relevant Targets Planned by Computer and Executed in the Laboratory

Klucznik, T., Mikulak-Klucznik, B., McCormack, M. P., Lima, H., Szymkuć, S., Bhowmick, M., Molga, K., Zhou, Y., Rickershauser, L., Gajewska, E. P., Toutchkine, A., Dittwald, P., Startek, M. P., Kirkovits, G. J., Roszak, R., Adamski, A., Sieredzińska, B., Mrksich, M., Trice, S. L. J. & Grzybowski, B. A., Mar 8 2018, In : Chem. 4, 3, p. 522-532 11 p.

Research output: Contribution to journalArticle

73 Scopus citations

Erratum to: Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery (Nature Communications, (2018), 9, 1, (2686), 10.1038/s41467-018-05110-x)

Jaroentomeechai, T., Stark, J. C., Natarajan, A., Glasscock, C. J., Yates, L. E., Hsu, K. J., Mrksich, M., Jewett, M. C. & DeLisa, M. P., Dec 1 2018, In : Nature communications. 9, 1, 3396.

Research output: Contribution to journalComment/debate

5 Scopus citations

High-Throughput Enzyme Kinetics with 3D Microfluidics and Imaging SAMDI Mass Spectrometry

Grant, J., Goudarzi, S. H. & Mrksich, M., Nov 6 2018, In : Analytical Chemistry. 90, 21, p. 13096-13103 8 p.

Research output: Contribution to journalArticle

3 Scopus citations

How many human proteoforms are there?

Aebersold, R., Agar, J. N., Amster, I. J., Baker, M. S., Bertozzi, C. R., Boja, E. S., Costello, C. E., Cravatt, B. F., Fenselau, C., Garcia, B. A., Ge, Y., Gunawardena, J., Hendrickson, R. C., Hergenrother, P. J., Huber, C. G., Ivanov, A. R., Jensen, O. N., Jewett, M. C., Kelleher, N. L., Kiessling, L. L. & 33 others, Krogan, N. J., Larsen, M. R., Loo, J. A., Ogorzalek Loo, R. R., Lundberg, E., Maccoss, M. J., Mallick, P., Mootha, V. K., Mrksich, M., Muir, T. W., Patrie, S. M., Pesavento, J. J., Pitteri, S. J., Rodriguez, H., Saghatelian, A., Sandoval, W., Schlüter, H., Sechi, S., Slavoff, S. A., Smith, L. M., Snyder, M. P., Thomas, P. M., Uhlén, M., Van Eyk, J. E., Vidal, M., Walt, D. R., White, F. M., Williams, E. R., Wohlschlager, T., Wysocki, V. H., Yates, N. A., Young, N. L. & Zhang, B., Feb 14 2018, In : Nature Chemical Biology. 14, 3, p. 206-214 9 p.

Research output: Contribution to journalReview article

132 Scopus citations

Long-Range Energy Transfer in Protein Megamolecules

Taylor, E. L., Metcalf, K. J., Carlotti, B., Lai, C. T., Modica, J. A., Schatz, G. C., Mrksich, M. & Goodson, T., Nov 21 2018, In : Journal of the American Chemical Society. 140, 46, p. 15731-15743 13 p.

Research output: Contribution to journalArticle

2 Scopus citations

Peptide Arrays: Development and Application

Szymczak, L. C., Kuo, H. Y. & Mrksich, M., Jan 2 2018, In : Analytical Chemistry. 90, 1, p. 266-282 17 p.

Research output: Contribution to journalReview article

26 Scopus citations

Photoactivatable Reaction for Covalent Nanoscale Patterning of Multiple Proteins

Zhou, S., Metcalf, K. J., Bugga, P., Grant, J. & Mrksich, M., Nov 28 2018, In : ACS Applied Materials and Interfaces. 10, 47, p. 40452-40459 8 p.

Research output: Contribution to journalArticle

1 Scopus citations
29 Scopus citations

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

Jaroentomeechai, T., Stark, J. C., Natarajan, A., Glasscock, C. J., Yates, L. E., Hsu, K. J., Mrksich, M., Jewett, M. C. & Delisa, M. P., Dec 1 2018, In : Nature communications. 9, 1, 2686.

Research output: Contribution to journalArticle

52 Scopus citations

Synthesis of Cyclic Megamolecules

Modica, J. A., Lin, Y. & Mrksich, M., May 23 2018, In : Journal of the American Chemical Society. 140, 20, p. 6391-6399 9 p.

Research output: Contribution to journalArticle

9 Scopus citations

Traceless Immobilization of Analytes for High-Throughput Experiments with SAMDI Mass Spectrometry

Helal, K. Y., Alamgir, A., Berns, E. J. & Mrksich, M., Jul 5 2018, In : Journal of the American Chemical Society. 140, 26, p. 8060-8063 4 p.

Research output: Contribution to journalArticle

4 Scopus citations
2017

A Bottom-Up Proteomic Approach to Identify Substrate Specificity of Outer-Membrane Protease OmpT

Wood, S. E., Sinsinbar, G., Gudlur, S., Nallani, M., Huang, C. F., Liedberg, B. & Mrksich, M., Dec 22 2017, In : Angewandte Chemie - International Edition. 56, 52, p. 16531-16535 5 p.

Research output: Contribution to journalArticle

17 Scopus citations

Active Site Metal Identity Alters Histone Deacetylase 8 Substrate Selectivity: A Potential Novel Regulatory Mechanism

Castaneda, C. A., Lopez, J. E., Joseph, C. G., Scholle, M. D., Mrksich, M. & Fierke, C. A., Oct 24 2017, In : Biochemistry. 56, 42, p. 5663-5670 8 p.

Research output: Contribution to journalArticle

10 Scopus citations

An Assay Based on SAMDI Mass Spectrometry for Profiling Protein Interaction Domains

O'Kane, P. T. & Mrksich, M., Aug 2 2017, In : Journal of the American Chemical Society. 139, 30, p. 10320-10327 8 p.

Research output: Contribution to journalArticle

7 Scopus citations

Bifunctional conjugates with potent inhibitory activity towards cyclooxygenase and histone deacetylase

Raji, I., Yadudu, F., Janeira, E., Fathi, S., Szymczak, L., Kornacki, J. R., Komatsu, K., Li, J. D., Mrksich, M. & Oyelere, A. K., 2017, In : Bioorganic and Medicinal Chemistry. 25, 3, p. 1202-1218 17 p.

Research output: Contribution to journalArticle

9 Scopus citations

Design of protein glycosylation sites by cell-free protein synthesis and mass spectrometry of self-assembled monolayers

Kightlinger, W., Lin, L., Mrksich, M. & Jewett, M. C., Jan 1 2017, Synthetic Biology Conference, SEED 2017: Engineering, Evolution, and Design. AIChE, p. 10-11 2 p. (Synthetic Biology Conference, SEED 2017: Engineering, Evolution, and Design).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Machine Learning on Signal-to-Noise Ratios Improves Peptide Array Design in SAMDI Mass Spectrometry

Xue, A. Y., Szymczak, L. C., Mrksich, M. & Bagheri, N., Sep 5 2017, In : Analytical Chemistry. 89, 17, p. 9039-9047 9 p.

Research output: Contribution to journalArticle

5 Scopus citations

Nanopatterned Extracellular Matrices Enable Cell-Based Assays with a Mass Spectrometric Readout

Cabezas, M. D., Mirkin, C. A. & Mrksich, M., Mar 8 2017, In : Nano letters. 17, 3, p. 1373-1377 5 p.

Research output: Contribution to journalArticle

11 Scopus citations

Peptide delivery with poly(ethylene glycol) diacrylate microneedles through swelling effect

Liu, S., Yeo, D. C., Wiraja, C., Tey, H. L., Mrksich, M. & Xu, C., Sep 2017, In : Bioengineering & translational medicine. 2, 3, p. 258-267 10 p.

Research output: Contribution to journalArticle

2016

Bisboronic Acids for Selective, Physiologically Relevant Direct Glucose Sensing with Surface-Enhanced Raman Spectroscopy

Sharma, B., Bugga, P., Madison, L. R., Henry, A. I., Blaber, M. G., Greeneltch, N. G., Chiang, N., Mrksich, M., Schatz, G. C. & Van Duyne, R. P., Oct 26 2016, In : Journal of the American Chemical Society. 138, 42, p. 13952-13959 8 p.

Research output: Contribution to journalArticle

56 Scopus citations

Cell-Based Screening: Cellular Assays with a Molecular Endpoint Measured by SAMDI Mass Spectrometry (Small 28/2016)

Berns, E. J., Cabezas, M. D. & Mrksich, M., Jul 27 2016, In : Small. 1 p.

Research output: Contribution to journalComment/debate