• 22770 Citations
1970 …2019
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Research Output 1970 2019

2019

Thomas A. Steitz (1940-2018)

Uhlenbeck, O. C., Feb 1 2019, In : RNA (New York, N.Y.). 25, 2, p. 169-172 4 p.

Research output: Contribution to journalArticle

2018
3 Citations (Scopus)

Evolutionary tuning impacts the design of bacterial tRNAs for the incorporation of unnatural amino acids by ribosomes

Uhlenbeck, O. C. & Schrader, J. M., Oct 2018, In : Current Opinion in Chemical Biology. 46, p. 138-145 8 p.

Research output: Contribution to journalReview article

Transfer RNA
Ribosomes
Tuning
Amino Acids
Peptide Elongation Factor Tu
2015
10 Citations (Scopus)

In vitro selection of DNAs with an increased propensity to form small circles

Rosanio, G., Widom, J. & Uhlenbeck, O. C., Jan 1 2015, In : Biopolymers. 103, 6, p. 303-320 18 p.

Research output: Contribution to journalArticle

DNA
Base Pairing
Molecules
Nucleosomes
Libraries
2014
2 Citations (Scopus)

Labeled EF-Tus for rapid kinetic studies of pretranslocation complex formation

Liu, W., Kavaliauskas, D., Schrader, J. M., Poruri, K., Birkedal, V., Goldman, E., Jakubowski, H., Mandecki, W., Uhlenbeck, O. C., Knudsen, C. R., Goldman, Y. E. & Cooperman, B. S., Oct 17 2014, In : ACS chemical biology. 9, 10, p. 2421-2431 11 p.

Research output: Contribution to journalArticle

Peptide Elongation Factor Tu
Transfer RNA
Kinetics
Assays
GTP Phosphohydrolases
8 Citations (Scopus)

The interface between Escherichia coli elongation factor Tu and aminoacyl-tRNA

Yikilmaz, E., Chapman, S. J., Schrader, J. M. & Uhlenbeck, O. C., Sep 9 2014, In : Biochemistry. 53, 35, p. 5710-5720 11 p.

Research output: Contribution to journalArticle

Peptide Elongation Factor Tu
Transfer RNA
Escherichia coli
Ribosomes
RNA, Transfer, Tyr
2013
53 Citations (Scopus)

Chemical map of Schizosaccharomyces pombe reveals species-specific features in nucleosome positioning

Moyle-Heyrman, G., Zaichuk, T., Xi, L., Zhang, Q., Uhlenbeck, O. C., Holmgren, R., Widom, J. & Wang, J. P., Dec 10 2013, In : Proceedings of the National Academy of Sciences of the United States of America. 110, 50, p. 20158-20163 6 p.

Research output: Contribution to journalArticle

Schizosaccharomyces
Nucleosomes
Transcription Initiation Site
Saccharomyces cerevisiae
DNA
10 Citations (Scopus)

TRNA residues evolved to promote translational accuracy

Shepotinovskaya, I. & Uhlenbeck, O. C., Apr 1 2013, In : RNA. 19, 4, p. 510-516 7 p.

Research output: Contribution to journalArticle

Codon
Escherichia coli
Mutation
Anticodon
Transfer RNA
2012
13 Citations (Scopus)

Duplex destabilization by four ribosomal DEAD-box proteins

Garcia, I., Albring, M. J. & Uhlenbeck, O. C., Dec 18 2012, In : Biochemistry. 51, 50, p. 10109-10118 10 p.

Research output: Contribution to journalArticle

Adenosine Triphosphate
Proteins
RNA
Hydrolysis
Nucleotides
9 Citations (Scopus)

Histidine 66 in Escherichia coli elongation factor Tu selectively stabilizes aminoacyl-tRNAs

Chapman, S. J., Schrader, J. M. & Uhlenbeck, O. C., Jan 6 2012, In : Journal of Biological Chemistry. 287, 2, p. 1229-1234 6 p.

Research output: Contribution to journalArticle

Peptide Elongation Factor Tu
RNA, Transfer, Gly
Transfer RNA
Histidine
Escherichia coli
2011
2 Citations (Scopus)

Functional consequences of T-stem mutations in E. coli tRNA ThrUGU in vitro and in vivo

Saks, M. E., Sanderson, L. E., Choi, D. S., Crosby, C. M. & Uhlenbeck, O. C., Jun 1 2011, In : RNA. 17, 6, p. 1038-1047 10 p.

Research output: Contribution to journalArticle

Transfer RNA
RNA, Transfer, Thr
Escherichia coli
Mutation
Peptide Elongation Factor Tu
14 Citations (Scopus)

Is the sequence-specific binding of aminoacyl-tRNAs by EF-Tu universal among bacteria?

Schrader, J. M. & Uhlenbeck, O. C., Dec 1 2011, In : Nucleic acids research. 39, 22, p. 9746-9758 13 p.

Research output: Contribution to journalArticle

Peptide Elongation Factor Tu
Transfer RNA
Bacteria
Amino Acids
Base Pairing
54 Citations (Scopus)

Tuning the affinity of aminoacyl-tRNA to elongation factor Tu for optimal decoding

Schrader, J. M., Chapman, S. J. & Uhlenbeck, O. C., Mar 29 2011, In : Proceedings of the National Academy of Sciences of the United States of America. 108, 13, p. 5215-5220 6 p.

Research output: Contribution to journalArticle

Peptide Elongation Factor Tu
Transfer RNA
Ribosomes
Codon
Base Pairing
2010
7 Citations (Scopus)

Enhanced product stability in the hammerhead ribozyme

Shepotinovskaya, I. & Uhlenbeck, O. C., Jun 1 2010, In : Biochemistry. 49, 21, p. 4494-4500 7 p.

Research output: Contribution to journalArticle

Magnesium Chloride
Assays
Catalytic Domain
Stabilization
Phosphates
2009
41 Citations (Scopus)

A dominant negative mutant of the E. coli RNA helicase DbpA blocks assembly of the 50S ribosomal subunit

Sharpe Elles, L. M., Sykes, M. T., Williamson, J. R. & Uhlenbeck, O. C., Nov 16 2009, In : Nucleic acids research. 37, 19, p. 6503-6514 12 p.

Research output: Contribution to journalArticle

Peptidyl Transferases
RNA Helicases
Ribosome Subunits
Ribosomes
23S Ribosomal RNA
53 Citations (Scopus)

A sequence element that tunes Escherichia coli tRNAGGC Ala to ensure accurate decoding

Ledoux, S., Olejniczak, M. & Uhlenbeck, O. C., Apr 1 2009, In : Nature Structural and Molecular Biology. 16, 4, p. 359-364 6 p.

Research output: Contribution to journalArticle

Codon
Escherichia coli
Transfer RNA
Anticodon
Conserved Sequence
Biochemistry
Processing
Proteins
RNA-dependent ATPase
6 Citations (Scopus)

RNA biophysics has come of age

Uhlenbeck, O. C., Oct 26 2009, In : Biopolymers. 91, 10, p. 811-814 4 p.

Research output: Contribution to journalArticle

Biophysics
X ray crystallography
RNA
21 Citations (Scopus)

Specificity of the ribosomal A site for aminoacyl-tRNAs

Dale, T., Fahlman, R. P., Olejniczak, M. & Uhlenbeck, O. C., Mar 24 2009, In : Nucleic acids research. 37, 4, p. 1202-1210 9 p.

Research output: Contribution to journalArticle

Transfer RNA
Anticodon
Ribosomes
Amino Acids
Amino Acid-Specific Transfer RNA
42 Citations (Scopus)

Understanding the Sequence Specificity of tRNA Binding to Elongation Factor Tu using tRNA Mutagenesis

Schrader, J. M., Chapman, S. J. & Uhlenbeck, O. C., Mar 13 2009, In : Journal of Molecular Biology. 386, 5, p. 1255-1264 10 p.

Research output: Contribution to journalArticle

Peptide Elongation Factor Tu
Transfer RNA
Mutagenesis
RNA, Transfer, Phe
Base Pairing
2008
57 Citations (Scopus)

[3′-32P]-labeling tRNA with nucleotidyltransferase for assaying aminoacylation and peptide bond formation

Ledoux, S. & Uhlenbeck, O. C., Feb 1 2008, In : Methods. 44, 2, p. 74-80 7 p.

Research output: Contribution to journalArticle

Nucleotidyltransferases
Aminoacylation
Transfer RNA
Labeling
Amino Acids
30 Citations (Scopus)

Catalytic diversity of extended hammerhead ribozymes

Shepotinovskaya, I. V. & Uhlenbeck, O. C., Jul 8 2008, In : Biochemistry. 47, 27, p. 7034-7042 9 p.

Research output: Contribution to journalArticle

Satellite RNAs
Viroids
Magnesium
Rate constants
Assays
66 Citations (Scopus)

Different aa-tRNAs Are Selected Uniformly on the Ribosome

Ledoux, S. & Uhlenbeck, O. C., Jul 11 2008, In : Molecular cell. 31, 1, p. 114-123 10 p.

Research output: Contribution to journalArticle

Transfer RNA
Ribosomes
Thermodynamics
Escherichia coli
Codon
12 Citations (Scopus)

Differential RNA-dependent ATPase activities of four rRNA processing yeast DEAD-box proteins

Garcia, I. & Uhlenbeck, O. C., Nov 25 2008, In : Biochemistry. 47, 47, p. 12562-12573 12 p.

Research output: Contribution to journalArticle

Yeast
Yeasts
Processing
Proteins
Ribosomes
44 Citations (Scopus)

Hammerhead redux: Does the new structure fit the old biochemical data?

Nelson, J. A. & Uhlenbeck, O. C., Apr 1 2008, In : RNA. 14, 4, p. 605-615 11 p.

Research output: Contribution to journalReview article

Dexfenfluramine
Schistosoma mansoni
hammerhead ribozyme
36 Citations (Scopus)

Minimal and extended hammerheads utilize a similar dynamic reaction mechanism for catalysis

Nelson, J. A. & Uhlenbeck, O. C., Jan 1 2008, In : RNA. 14, 1, p. 43-54 12 p.

Research output: Contribution to journalArticle

Catalysis
Base Pairing
Mutation
Schistosoma mansoni
Catalytic Domain
35 Citations (Scopus)
Staphylococcal Protein A
Fingers
Adenosine Triphosphatases
Arginine
Catalytic Domain
2007
20 Citations (Scopus)

Directed Mutagenesis Identifies Amino Acid Residues Involved in Elongation Factor Tu Binding to yeast Phe-tRNAPhe

Sanderson, L. E. & Uhlenbeck, O. C., Apr 20 2007, In : Journal of Molecular Biology. 368, 1, p. 119-130 12 p.

Research output: Contribution to journalArticle

Peptide Elongation Factor Tu
RNA, Transfer, Phe
Transfer RNA
Mutagenesis
Yeasts
16 Citations (Scopus)

Exploring the specificity of bacterial elongation factor Tu for different tRNAs

Sanderson, L. E. & Uhlenbeck, O. C., May 29 2007, In : Biochemistry. 46, 21, p. 6194-6200 7 p.

Research output: Contribution to journalArticle

Peptide Elongation Factor Tu
Transfer RNA
Amino Acid-Specific Transfer RNA
Amino Acids
Thermus thermophilus
29 Citations (Scopus)

The 51-63 base pair of tRNA confers specificity for binding by EF-Tu

Sanderson, L. E. & Uhlenbeck, O. C., Jun 1 2007, In : RNA. 13, 6, p. 835-840 6 p.

Research output: Contribution to journalArticle

Peptide Elongation Factor Tu
Transfer RNA
Base Pairing
Guanine
Thermus thermophilus
2006
6 Citations (Scopus)

Alanine scanning of MS2 coat protein reveals protein-phosphate contacts involved in thermodynamic hot spots

Hobson, D. & Uhlenbeck, O. C., Feb 24 2006, In : Journal of Molecular Biology. 356, 3, p. 613-624 12 p.

Research output: Contribution to journalArticle

Capsid Proteins
Thermodynamics
Alanine
Phosphates
RNA
16 Citations (Scopus)

Quantitative analysis of deoxynucleotide substitutions in the codon-anticodon helix

Fahlman, R. P., Olejniczak, M. & Uhlenbeck, O. C., Feb 3 2006, In : Journal of Molecular Biology. 355, 5, p. 887-892 6 p.

Research output: Contribution to journalArticle

Anticodon
Codon
Binding Sites
Transfer RNA
Paromomycin
50 Citations (Scopus)

The domain of the Bacillus subtilis DEAD-box helicase YxiN that is responsible for specific binding of 23S rRNA has an RNA recognition motif fold

Wang, S., Hu, Y., Overgaard, M. T., Karginov, F. V., Uhlenbeck, O. C. & McKay, D. B., Jun 1 2006, In : RNA. 12, 6, p. 959-967 9 p.

Research output: Contribution to journalArticle

Bacillus subtilis
RNA
23S Ribosomal RNA
DEAD-box RNA Helicases
Ribonucleoproteins
45 Citations (Scopus)

tRNA residues that have coevolved with their anticodon to ensure uniform and accurate codon recognition

Olejniczak, M. & Uhlenbeck, O. C., Aug 1 2006, In : Biochimie. 88, 8, p. 943-950 8 p.

Research output: Contribution to journalArticle

Transfer RNA
Codon
Anticodon
Phylogeny
Base Pairing
46 Citations (Scopus)

When to Believe What You See

Nelson, J. A. & Uhlenbeck, O. C., Aug 18 2006, In : Molecular Cell. 23, 4, p. 447-450 4 p.

Research output: Contribution to journalShort survey

Catalytic Domain
Schistosoma mansoni
X-Rays
RNA
hammerhead ribozyme
2005
54 Citations (Scopus)

Amino acid specificity in translation

Dale, T. & Uhlenbeck, O. C., Dec 2005, In : Trends in Biochemical Sciences. 30, 12, p. 659-665 7 p.

Research output: Contribution to journalArticle

Transfer RNA
Amino Acids
Amino Acid-Specific Transfer RNA
Peptide Elongation Factor Tu
Substrates
11 Citations (Scopus)

Binding of misacylated tRNAs to the ribosomal a site

Dale, T. & Uhlenbeck, O. C., Nov 1 2005, In : RNA. 11, 11, p. 1610-1615 6 p.

Research output: Contribution to journalArticle

Transfer RNA
Amino Acid-Specific Transfer RNA
Ribosomes
Anticodon
Valine
27 Citations (Scopus)

Escherichia coli DbpA Is a 3′ → 5′ RNA helicase

Diges, C. M. & Uhlenbeck, O. C., May 31 2005, In : Biochemistry. 44, 21, p. 7903-7911 9 p.

Research output: Contribution to journalArticle

RNA Helicases
Escherichia coli
RNA
Substrates
38 Citations (Scopus)

Hammerheads derived from sTRSV show enhanced cleavage and ligation rate constants

Nelson, J. A., Shepotinovskaya, I. & Uhlenbeck, O. C., Nov 8 2005, In : Biochemistry. 44, 44, p. 14577-14585 9 p.

Research output: Contribution to journalArticle

Magnesium
Ligation
Rate constants
Genes
Magnesium Chloride
70 Citations (Scopus)

Idiosyncratic tuning of tRNAs to achieve uniform ribosome binding

Olejniczak, M., Dale, T., Fahlman, R. P. & Uhlenbeck, O. C., Sep 1 2005, In : Nature Structural and Molecular Biology. 12, 9, p. 788-793 6 p.

Research output: Contribution to journalArticle

Transfer RNA
Ribosomes
Anticodon
Codon
Mutagenesis
37 Citations (Scopus)

Predicting the binding affinities of misacylated tRNAs for Thermus thermophilus EF-Tu·GTP

Asahara, H. & Uhlenbeck, O. C., Aug 23 2005, In : Biochemistry. 44, 33, p. 11254-11261 8 p.

Research output: Contribution to journalArticle

Thermus thermophilus
Peptide Elongation Factor Tu
Transfer RNA
Free energy
Escherichia coli
119 Citations (Scopus)

The structure-function dilemma of the hammerhead ribozyme

Blount, K. F. & Uhlenbeck, O. C., Jun 30 2005, In : Annual Review of Biophysics and Biomolecular Structure. 34, p. 415-440 26 p.

Research output: Contribution to journalReview article

Catalysis
Functional groups
Enzymes
Catalytic RNA
Essential Amino Acids
52 Citations (Scopus)

YxiN is a modular protein combining a DExD/H core and a specific RNA-binding domain

Karginov, F. V., Caruthers, J. M., Hu, Y. X., McKay, D. B. & Uhlenbeck, O. C., Oct 21 2005, In : Journal of Biological Chemistry. 280, 42, p. 35499-35505 7 p.

Research output: Contribution to journalArticle

RNA
Proteins
RNA Helicases
Ribosomal RNA
Bacilli
2004
39 Citations (Scopus)

Contribution of the esterified amino acid to the binding of aminoacylated tRNAs to the ribosomal P- and A-sites

Fahlman, R. P. & Uhlenbeck, O. C., Jun 15 2004, In : Biochemistry. 43, 23, p. 7575-7583 9 p.

Research output: Contribution to journalArticle

Transfer RNA
Amino Acids
Phenylalanine
Amino Acid-Specific Transfer RNA
Ribosomes
1 Citation (Scopus)

Hasty decisions on the ribosome

Sherlin, L. D. & Uhlenbeck, O. C., Mar 1 2004, In : Nature Structural and Molecular Biology. 11, 3, p. 206-208 3 p.

Research output: Contribution to journalShort survey

Transfer RNA
Ribosomes
Proteins
21 Citations (Scopus)

Interaction of Escherichia coli DbpA with 23S rRNA in different functional states of the enzyme

Karginov, F. V. & Uhlenbeck, O. C., Aug 16 2004, In : Nucleic acids research. 32, 10, p. 3028-3032 5 p.

Research output: Contribution to journalArticle

RNA
Escherichia coli
ribonuclease T(2)
Enzymes
Adenylyl Imidodiphosphate
71 Citations (Scopus)

The affinity of elongation factor Tu for an aminoacyl-tRNA is modulated by the esterified amino acid

Dale, T., Sanderson, L. E. & Uhlenbeck, O. C., May 25 2004, In : Biochemistry. 43, 20, p. 6159-6166 8 p.

Research output: Contribution to journalArticle

Peptide Elongation Factor Tu
Transfer RNA
Amino Acids
RNA, Transfer, Phe
Thermus thermophilus
75 Citations (Scopus)

Uniform binding of aminoacylated transfer RNAs to the ribosomal A and P sites

Fahlman, R. P., Dale, T. & Uhlenbeck, O. C., Dec 3 2004, In : Molecular cell. 16, 5, p. 799-805 7 p.

Research output: Contribution to journalArticle

Transfer RNA
Ribosomes
Amino Acid-Specific Transfer RNA
Escherichia coli
Anticodon
2003
18 Citations (Scopus)

Less isn't always more

Uhlenbeck, O. C., Dec 1 2003, In : RNA. 9, 12, p. 1415-1417 3 p.

Research output: Contribution to journalComment/debate

Viroids
Nucleic Acid Conformation
Catalytic RNA
RNA
2002
57 Citations (Scopus)

Cooperative binding of ATP and RNA substrates to the DEAD/H protein DbpA

Polach, K. J. & Uhlenbeck, O. C., Mar 19 2002, In : Biochemistry. 41, 11, p. 3693-3702 10 p.

Research output: Contribution to journalArticle

Adenosine Triphosphate
RNA
Assays
Substrates
Proteins
24 Citations (Scopus)

Internal equilibrium of the hammerhead ribozyme is altered by the length of certain covalent cross-links

Blount, K. F. & Uhlenbeck, O. C., May 28 2002, In : Biochemistry. 41, 21, p. 6834-6841 8 p.

Research output: Contribution to journalArticle

Ligation
RNA
hammerhead ribozyme