Pamela J Focia

  • 1855 Citations
19952018
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Research Output 1995 2016

  • 1855 Citations
  • 37 Article
  • 2 Comment/debate
2016
14 Citations (Scopus)

Structural insights into Parkin substrate lysine targeting from minimal Miro substrates

Klosowiak, J. L., Park, S., Smith, K. P., French, M. E., Focia, P. J., Freymann, D. M. & Rice, S. E., Sep 8 2016, In : Scientific Reports. 6, 33019.

Research output: Contribution to journalArticle

Lysine
Ubiquitination
GTP Phosphohydrolases
Ubiquitin
Inborn Genetic Diseases
2014
12 Citations (Scopus)

Structures of KcsA in complex with symmetrical quaternary ammonium compounds reveal a hydrophobic binding site

Lenaeus, M. J., Burdette, D., Wagner, T., Focia, P. J. & Gross, A., Aug 19 2014, In : Biochemistry. 53, 32, p. 5365-5373 9 p.

Research output: Contribution to journalArticle

Quaternary Ammonium Compounds
Potassium Channels
Binding Sites
Membranes
Sodium Channels
2013
31 Citations (Scopus)

Structural coupling of the EF hand and C-terminal GTPase domains in the mitochondrial protein Miro

Klosowiak, J. L., Focia, P. J., Chakravarthy, S., Landahl, E. C., Freymann, D. M. & Rice, S. E., Nov 1 2013, In : EMBO Reports. 14, 11, p. 968-974 7 p.

Research output: Contribution to journalArticle

EF Hand Motifs
GTP Phosphohydrolases
Mitochondrial Proteins
Phosphorylation
Nucleotides
2012
13 Citations (Scopus)

Structure of a tetrameric galectin from Cinachyrella sp. (ball sponge)

Freymann, D. M., Nakamura, Y., Focia, P. J., Sakai, R. & Swanson, G. T., Sep 1 2012, In : Acta Crystallographica Section D: Biological Crystallography. 68, 9, p. 1163-1174 12 p.

Research output: Contribution to journalArticle

Galectin 1
Galectins
Ionotropic Glutamate Receptors
Porifera
Aptitude
2011
31 Citations (Scopus)

Homophilic adhesion mechanism of neurofascin, a member of the L1 family of neural cell adhesion molecules

Liu, H., Focia, P. J. & He, X., Jan 7 2011, In : Journal of Biological Chemistry. 286, 1, p. 797-805 9 p.

Research output: Contribution to journalArticle

Neural Cell Adhesion Molecule L1
Neural Cell Adhesion Molecules
Adhesion
Cell Adhesion Molecules
Mutagenesis
2010
26 Citations (Scopus)

Electron spin-echo envelope modulation (ESEEM) reveals water and phosphate interactions with the KcsA potassium channel

Cieslak, J. A., Focia, P. J. & Gross, A., Feb 23 2010, In : Biochemistry. 49, 7, p. 1486-1494 9 p.

Research output: Contribution to journalArticle

Potassium Channels
Phosphates
Modulation
Electrons
Water
87 Citations (Scopus)

Structural Basis of Semaphorin-Plexin Recognition and Viral Mimicry from Sema7A and A39R Complexes with PlexinC1

Liu, H., Juo, Z. S., Shim, A. H. R., Focia, P. J., Chen, X., Garcia, K. C. & He, X., Sep 3 2010, In : Cell. 142, 5, p. 749-761 13 p.

Research output: Contribution to journalArticle

Semaphorins
Propellers
Variola virus
Cardiovascular system
Dimerization
83 Citations (Scopus)

Structures of a platelet-derived growth factor/propeptide complex and a platelet-derived growth factor/receptor complex

Shim, A. H. R., Liu, H., Focia, P. J., Chen, X., Lin, P. C. & He, X., Jun 22 2010, In : Proceedings of the National Academy of Sciences of the United States of America. 107, 25, p. 11307-11312 6 p.

Research output: Contribution to journalArticle

Platelet-Derived Growth Factor Receptors
Platelet-Derived Growth Factor
Proto-Oncogene Proteins c-sis
Cystine
Growth Factor Receptors
2008
156 Citations (Scopus)

Cu(I) recognition via cation-π and methionine interactions in CusF

Xue, Y., Davis, A. V., Balakrishnan, G., Stasser, J. P., Staehlin, B. M., Focia, P. J., Spiro, T. G., Penner-Hahn, J. E. & O'Halloran, T. V., Jan 1 2008, In : Nature Chemical Biology. 4, 2, p. 107-109 3 p.

Research output: Contribution to journalArticle

Methionine
Cations
Metals
Tryptophan
Sulfides
3 Citations (Scopus)

Nucleotide-binding flexibility in ultrahigh-resolution structures of the SRP GTPase Ffh

Ramirez, U. D., Focia, P. J. & Freymann, D. M., Sep 19 2008, In : Acta Crystallographica Section D: Biological Crystallography. 64, 10, p. 1043-1053 11 p.

Research output: Contribution to journalArticle

Signal Recognition Particle
GTP Phosphohydrolases
Nucleotides
Catalytic Domain
Guanine
65 Citations (Scopus)

Structural basis for synaptic adhesion mediated by neuroligin-neurexin interactions

Chen, X., Liu, H., Shim, A. H. R., Focia, P. J. & He, X., Jan 1 2008, In : Nature Structural and Molecular Biology. 15, 1, p. 50-56 7 p.

Research output: Contribution to journalArticle

Alternative Splicing
Sex Hormone-Binding Globulin
Mutation
Cholinesterases
Brain Diseases
50 Citations (Scopus)
Macrophage Colony-Stimulating Factor
Receptor Protein-Tyrosine Kinases
Ligands
Dimerization
Phagocytes
2007
75 Citations (Scopus)

Structural basis for stem cell factor-KIT signaling and activation of class III receptor tyrosine kinases

Liu, H., Chen, X., Focia, P. J. & He, X., Feb 7 2007, In : EMBO Journal. 26, 3, p. 891-901 11 p.

Research output: Contribution to journalArticle

Stem Cell Factor
Receptor Protein-Tyrosine Kinases
Chemical activation
Immunoglobulins
Epitopes
21 Citations (Scopus)

X-ray structure of the T. aquaticus Ftsy:GDP complex suggests functional roles for the C-terminal helix of the SRP GTPases

Gawronski-Salerno, J., Coon V, J. S., Focia, P. J. & Freymann, D. M., Mar 1 2007, In : Proteins: Structure, Function and Genetics. 66, 4, p. 984-995 12 p.

Research output: Contribution to journalArticle

Signal Recognition Particle
GTP Phosphohydrolases
X-Rays
X rays
Protein Transport
2006
24 Citations (Scopus)

Structure of a GDP:AlF4 Complex of the SRP GTPases Ffh and FtsY, and Identification of a Peripheral Nucleotide Interaction Site

Focia, P. J., Gawronski-Salerno, J., Coon V, J. S. & Freymann, D. M., Jul 14 2006, In : Journal of Molecular Biology. 360, 3, p. 631-643 13 p.

Research output: Contribution to journalArticle

Signal Recognition Particle
GTP Phosphohydrolases
Nucleotides
Guanosine Triphosphate
Conserved Sequence
2005
104 Citations (Scopus)

Structural basis of TEA blockade in a model potassium channel

Lenaeus, M. J., Vamvouka, M., Focia, P. J. & Gross, A., May 1 2005, In : Nature Structural and Molecular Biology. 12, 5, p. 454-459 6 p.

Research output: Contribution to journalArticle

Tetraethylammonium
Potassium Channels
Potassium
Dehydration
Membrane Potentials
2004
200 Citations (Scopus)

Heterodimeric GTPase Core of the SRP Targeting Complex

Focia, P. J., Shepotinovskaya, I. V., Seidler, J. A. & Freymann, D. M., Jan 16 2004, In : Science. 303, 5656, p. 373-377 5 p.

Research output: Contribution to journalArticle

Signal Recognition Particle
Guanosine
Catalytic Domain
GTP Phosphohydrolases
Protein Transport
25 Citations (Scopus)

Interactions at the Dimer Interface Influence the Relative Efficiencies for Purine Nucleotide Synthesis and Pyrophosphorolysis in a Phosphoribosyltransferase

Canyuk, B., Medrano, F. J., Wenck, M. A., Focia, P. J., Eakin, A. E. & Craig, S. P., Jan 23 2004, In : Journal of Molecular Biology. 335, 4, p. 905-921 17 p.

Research output: Contribution to journalArticle

Purine Nucleotides
Hypoxanthine Phosphoribosyltransferase
Inosine Monophosphate
Catalysis
Purinones
14 Citations (Scopus)

Novel Protein and Mg2+ Configurations in the Mg2+GDP Complex of the SRP GTPase Ffh

Focia, P. J., Alam, H., Lu, T., Ramirez, U. D. & Freymann, D. M., Feb 1 2004, In : Proteins: Structure, Function and Genetics. 54, 2, p. 222-230 9 p.

Research output: Contribution to journalArticle

Signal Recognition Particle
GTP Phosphohydrolases
Magnesium
Conformations
Guanosine Triphosphate
2003
69 Citations (Scopus)

An aminopyridazine-based inhibitor of a pro-apoptotic protein kinase attenuates hypoxia-ischemia induced acute brain injury

Velentza, A. V., Wainwright, M., Zasadzki, M., Mirzoeva, S., Schumacher, A. M., Haiech, J., Focia, P. J., Egli, M. & Watterson, D. M., Oct 20 2003, In : Bioorganic and Medicinal Chemistry Letters. 13, 20, p. 3465-3470 6 p.

Research output: Contribution to journalArticle

Death-Associated Protein Kinases
Apoptosis Regulatory Proteins
Brain Injuries
Protein Kinases
Brain
11 Citations (Scopus)

Crystallization of the GMPPCP complex of the NG domains of Thermus aquaticus Ffh and FtsY

Shepotinovskaya, I. V., Focia, P. J. & Freymann, D. M., Oct 1 2003, In : Acta Crystallographica - Section D Biological Crystallography. 59, 10, p. 1834-1837 4 p.

Research output: Contribution to journalArticle

Thermus
GTP Phosphohydrolases
Crystallization
Guanosine Triphosphate
Signal Recognition Particle
94 Citations (Scopus)

Nanomolar inhibitors of AmpC β-lactamase

Morandi, F., Caselli, E., Morandi, S., Focia, P. J., Blázquez, J., Shoichet, B. K. & Prati, F., Jan 22 2003, In : Journal of the American Chemical Society. 125, 3, p. 685-695 11 p.

Research output: Contribution to journalArticle

Lactams
X ray crystallography
X Ray Crystallography
Antibiotics
Cephalosporins
2002
1 Citation (Scopus)
Cephalothin
Hydrolases
Amides
AmpC beta-lactamases
32 Citations (Scopus)

Structural basis for mobility in the 1.1 Å crystal structure of the NG domain of Thermus aquaticus Ffh

Ramirez, U. D., Minasov, G., Focia, P. J., Stroud, R. M., Walter, P., Kuhn, P. & Freymann, D. M., Jan 1 2002, In : Journal of Molecular Biology. 320, 4, p. 783-799 17 p.

Research output: Contribution to journalArticle

Thermus
GTP Phosphohydrolases
Conserved Sequence
Signal Recognition Particle
Protein Transport
94 Citations (Scopus)

Structural milestones in the reaction pathway of an amide hydrolase: Substrate, acyl, and product complexes of cephalothin with AmpC β-lactamase

Beadle, B. M., Trehan, I., Focia, P. J. & Shoichet, B. K., Mar 26 2002, In : Structure. 10, 3, p. 413-424 12 p.

Research output: Contribution to journalArticle

Cephalothin
Hydrolases
Amides
Lactams
Enzymes
2001
87 Citations (Scopus)

Structures of ceftazidime and its transition-state analogue in complex with AmpC β-lactamase: Implications for resistance mutations and inhibitor design

Powers, R. A., Caselli, E., Focia, P. J., Prati, F. & Shoichet, B. K., Aug 7 2001, In : Biochemistry. 40, 31, p. 9207-9214 8 p.

Research output: Contribution to journalArticle

Ceftazidime
Cephalosporins
Mutation
Conformations
Lactams
19 Citations (Scopus)
Hypoxanthine Phosphoribosyltransferase
Mutagenesis
Functional analysis
X ray crystallography
X Ray Crystallography
2000
58 Citations (Scopus)

Efficient identification of inhibitors targeting the closed active site conformation of the HPRT from Trypanosoma cruzi

Freymann, D. M., Wenck, M. A., Engel, J. C., Feng, J., Focia, P. J., Eakin, A. E. & Craig, S. P., Jan 1 2000, In : Chemistry and Biology. 7, 12, p. 957-968 12 p.

Research output: Contribution to journalArticle

Hypoxanthine Phosphoribosyltransferase
Trypanosoma cruzi
Conformations
Catalytic Domain
Parasites
1999
1 Citation (Scopus)

Erratum: Ternary complex structure of human HGPRTase, PRPP, Mg2+, and the inhibitor HPP reveals the involvement of the flexible loop in substrate binding (Protein Science (1999) 8:5 (1023-1031))

Balendiran, G. K., Molina, J. A., Xu, Y., Torres-Martinez, J., Stevens, R., Focia, P. J., Eakin, A. E., Sacchettini, J. C. & Craig, S. P., Dec 1 1999, In : Protein Science. 8, 12, 1 p.

Research output: Contribution to journalComment/debate

Carrier Proteins
Substrates
N-(4'-fluorobutyrophenone)-4-(4-chlorophenyl)pyridinium
54 Citations (Scopus)

Ternary complex structure of human HGPRTase, PRPP, Mg2+, and the inhibitor HPP reveals the involvement of the flexible loop in substrate binding

Balendiran, G. K., Molina, J. A., Xu, Y., Torres-Martinez, J., Stevens, R., Focia, P. J., Eakin, A. E., Sacchettini, J. C. & Craig, S. P., Jan 1 1999, In : Protein Science. 8, 5, p. 1023-1031 9 p.

Research output: Contribution to journalArticle

Phosphoribosyl Pyrophosphate
Substrates
Enzymes
Gouty Arthritis
Hypoxanthine Phosphoribosyltransferase
1998
53 Citations (Scopus)

A 1.4 Å Crystal structure for the hypoxanthine phosphoribosyltransferase of Trypanosoma cruzi

Focia, P. J., Craig, S. P., Nieves-Alicea, R., Fletterick, R. J. & Eakin, A. E., Oct 27 1998, In : Biochemistry. 37, 43, p. 15066-15075 10 p.

Research output: Contribution to journalArticle

Hypoxanthine Phosphoribosyltransferase
Trypanosoma cruzi
Catalytic Domain
Crystal structure
Dimers
71 Citations (Scopus)

Approaching the transition state in the crystal structure of a phosphoribosyltransferase

Focia, P. J., Craig, S. P. & Eakin, A. E., Dec 8 1998, In : Biochemistry. 37, 49, p. 17120-17127 8 p.

Research output: Contribution to journalArticle

Hypoxanthine Phosphoribosyltransferase
Crystal structure
Trypanosoma cruzi
Ions
Purinones
6 Citations (Scopus)
Proteolysis
Hypoxanthine Phosphoribosyltransferase
X-Rays
X rays
Crystals
1997

Crystallographic studies of parasite hgprtases pamela

Focia, J., Somoza, J. R. & Freymann, D. M., Dec 1 1997, In : FASEB Journal. 11, 9

Research output: Contribution to journalArticle

Hypoxanthine Phosphoribosyltransferase
Parasites
Enzymes
Crystal structure
Purine Nucleotides
50 Citations (Scopus)

Hypoxanthine phosphoribosyltransferase from Trypanosoma cruzi as a target for structure-based inhibitor design: Crystallization and inhibition studies with purine analogs

Eakin, A. E., Guerra, A., Focia, P. J., Torres-Martinez, J. & Craig, S. P., Aug 1 1997, In : Antimicrobial Agents and Chemotherapy. 41, 8, p. 1686-1692 7 p.

Research output: Contribution to journalArticle

Hypoxanthine Phosphoribosyltransferase
Trypanosoma cruzi
Crystallization
Enzymes
Purine Nucleotides
6 Citations (Scopus)
Phosphoribosyl Pyrophosphate
Hypoxanthine Phosphoribosyltransferase
Lysine
Arginine
Catalytic Domain

The crystal structure of the hypoxanthine-guanine phosphoribosyltransferase (HGPRT) from trypanosoma cruzi

Eakin, A. E., Focia, P. J., Nieves-Alicea, R., Craig, S. P. & Fletterick, R. J., Dec 1 1997, In : Protein Engineering. 10, 3333, 1 p.

Research output: Contribution to journalArticle

Hypoxanthine Phosphoribosyltransferase
Trypanosoma cruzi
Crystal structure
1996
101 Citations (Scopus)

Crystal structure of the hypoxanthine-guanine-xanthine phosphoribosyltransferase from the protozoan parasite Tritrichomonas foetus

Somoza, J. R., Chin, M. S., Focia, P. J., Wang, C. C. & Fletterick, R. J., Jun 4 1996, In : Biochemistry. 35, 22, p. 7032-7040 9 p.

Research output: Contribution to journalArticle

Tritrichomonas foetus
Parasites
Crystal structure
Hinges
Molecules
1995
14 Citations (Scopus)

Identification of the Active Sites of Human and Schistosomal Hypoxanthine-Guanine Phosphoribosyltransferases by GMP-2’,3'-dialdehyde Affinity Labeling

Kanaani, J., Maltby, D., Focia, P. & Wang, C. C., Jan 1 1995, In : Biochemistry. 34, 46, p. 14987-14996 10 p.

Research output: Contribution to journalArticle

Hypoxanthine Phosphoribosyltransferase
Labeling
Catalytic Domain
Enzymes
Iodoacetates