• 2997 Citations
1973 …2018
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Personal profile

Research Interests

The Sonic hedgehog signal in vertebrates is mediated by three C2H2 zinc finger transcription factors, GLI1, GLI2, and GLI3. We have shown that near identity of gene sequence exists between mouse and human GLI1 and that GLI1 functions as a transcription factor and that a critical domain in the COOH end of the protein is a transactivator with VP16-like structure. We established that GLI1 protein regulates a set of genes that coordinately control proliferation and may in part explain malignant transformation by misexpression of GLI1. We have established non-canonical activation of GLI1 by c-myc short-circuiting patched and smoothened trans-membrane proteins in lymphoma. We established that GLI1 binds TAF9 at the gene target and competes with P53 for this adapter protein providing an explanation for GLI1 oncogenic activity. We are working out the mechanisms of autoregulation of GLI1 by molecular analysis of cis and trans effectors using gene editing techniques. We have established a strong intronic enhancer region that in part explains GLI autoregulation. We are establishing key protein-protein interactions during assembly of the transcription machinery. We have developed chimeras and a method to identify cell lineage in visceral organs. We established that chemically induced tumors and preneoplastic lesions are clonal, the first lab to do this. The patterns in normal tissue derive from the allocation of stem cells during development. We show that the patterns are fractal, likely arise from iterating simple division rules and do not require cell movement for complex, highly variegated patterns to form. In the cornea epithelial cells are assorted in spirals. Absence of the pattern is associated with Pax6 mutations and keratopathies. Using finite element models we have shown the patterns correlate with path lines of maximum stress/strain, suggesting that shearing forces are responsible for the pattern.

Education/Academic qualification

PhD, University of Oxford

… → 1977

MD, State University of New York

… → 1972

Keywords

  • Cancer Biology
  • Cell Biology
  • Cell Imaging
  • Embryology
  • Gene Regulation
  • Stem Cells

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Grants 2001 2007

Developmental Biology
Carcinogenesis
Education
Neoplasms
Research

Research Output 1973 2018

Application of small molecule CHIR99021 leads to the loss of hemangioblast progenitor and increased hematopoiesis of human pluripotent stem cells

Galat, Y., Elcheva, I., Dambaeva, S., Katukurundage, D., Beaman, K., Iannaccone, P. M. & Galat, V., Sep 1 2018, In : Experimental Hematology. 65, p. 38-48.e1

Research output: Contribution to journalArticle

Hemangioblasts
Pluripotent Stem Cells
Hematopoiesis
Cell Differentiation
Hematopoietic System

The utility of stem cells in pediatric urinary bladder regeneration

Iannaccone, P. M., Galat, V., Bury, M. I., Ma, Y. C. & Sharma, A. K., Jan 1 2018, In : Pediatric Research. 83, 1-2, p. 258-266 9 p.

Research output: Contribution to journalReview article

Regeneration
Urinary Bladder
Stem Cells
Pediatrics
Induced Pluripotent Stem Cells
3 Citations

Cytokine-free directed differentiation of human pluripotent stem cells efficiently produces hemogenic endothelium with lymphoid potential

Galat, Y., Dambaeva, S., Elcheva, I., Khanolkar, A., Beaman, K., Iannaccone, P. M. & Galat, V., Mar 17 2017, In : Stem Cell Research and Therapy. 8, 1, p. 1-11 11 p., 67.

Research output: Contribution to journalArticle

Hemangioblasts
Pluripotent Stem Cells
Stem cells
Cytokines
Hematopoiesis
5 Citations
Induced Pluripotent Stem Cells
Mesenchymal Stromal Cells
Transgenes
Lentivirus
Regenerative Medicine

Mechanics and spiral formation in the rat cornea

Mohammad, N. T., Iannaccone, S., Rutherford, W. & Foster, CD., Jan 2015, In : Biomech Model Mechanobiology. 14, 1, p. 107-122 16 p.

Research output: Contribution to journalArticle