Recently developed capabilities in three dimensional (3D) microsystem technologies enable, for the first time, the realization of 3D mesoscale constructs that incorporate active electronic, optoelectronic and microfluidic functionality in open, filamentary networks. These platforms will make it possible to create 3D in vitro models of dissociated neuron cultures that more closely resemble native cell-to-cell communication and architecture. Lithographically defined patterns of surface chemistry will allow for controlled growth of neurons and 3D neural circuits in ways that support electrophysiological, optogenetic and/or pharmacological interrogation at the single-cell level and across cell populations. The versatility and success of these new technologies will be shown through control of exogenous proteins that define signaling cascades and cell-to-cell communication. The stimulation or inhibition of a targeted signaling pathway within a single cell will be evaluated for its ability to change activity as measured by fluctuations in electrical communication from that cell, and subsequent changes in total network activity.
|Effective start/end date||5/15/19 → 5/14/22|
- Army Research Office (W911NF1920169-P00002)