Allergen loaded nanoparticles for food allergy tolerance

Project: Research project

Project Details

Description

This proposal is a continuation of the long-standing collaboration between the Shea and
Miller Laboratories examining tolerogenic therapy in Th1/17-mediated autoimmune diseases. The personnel in
my laboratory listed in the budget justification will carry out the in vivo mechanistic experiments detailed in
Aims 1 and 2 determining the the efficiency of tolerance of PLG particle formulations identified by Dr. Shea’s
laboratory for their ability to prevent and treat ongoing Th2-mediated models of food allergy induced by
immunization with either peanut (PN) or egg albumin (OVA) proteins. In addition, my lab will carry out the in
vivo and in vitro experiments detailed in Aim 2 examining the mechanisms (deletion, anergy, immune deviation
and T cell regulation) by which Th2 tolerance is induced and maintained using Ag-encapsulating PLG
nanoparticles (proliferation, ELISPOT, intracellular cytokine staining, etc.), and assays related to quantitation of
gut inflammation using flow cytometric analyses of peripheral and gut-infiltrating immune inflammatory cells
and staining of frozen gut sections. I will consult regularly with Drs. Shea and O’Konek and their lab personnel
at the University of Michigan via regular skype meetings and twice yearly in person meetings to review data
and plan experiments, as well as meeting daily with my lab personnel to discuss experimental design and
results.
StatusActive
Effective start/end date9/16/208/31/25

Funding

  • University of Michigan (SUBK00012728//1R01AI155678-01)
  • National Institute of Allergy and Infectious Diseases (SUBK00012728//1R01AI155678-01)

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