The proposed project provides us a unique opportunity to identify specific genes modifiers after bariatric surgery in breast tissue and elucidate molecular pathways associated with breast cancer reduction. We will perform exome capture RNA-seq on matched breast tissue samples obtained before and after bariatric surgery resulting in loss of 25% excess body weight. We hypothesize that the gene regulation pattern in the breast will be consistent with an overall antiestrogenic effect, with identification of possible key changes in expression of estrogen modulating and/or inflammatory markers, including ERα, CYP19, IGF-1, APN, TNF-α, IL-6, and leptin. From this approach we can also identify novel genes that may be altered due to bariatric surgery, thereby allowing us to explore previously unknown pathways related to breast cancer risk reduction. Additionally, we will able to compare differential gene expression in premenopausal and postmenopausal patients after bariatric surgery. Lastly, we can also measure changes in gene expression after bariatric surgery between those patients who had a purely restrictive procedure and those who had both a restrictive and malabsorptive procedure. These results will contribute to a better understanding of the molecular pathogenesis of breast cancer risk reduction through weight loss in obese patients across several different variables.
|Effective start/end date||9/1/17 → 8/31/19|
- Northwestern Memorial Hospital (Agmt 10/17/17)
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