Mapping the 3D architecture of native human replisomes

Dr. Yang will collaborate with Dr. Gilbert (the PI) at the San Diego Biomedical Research Institute as a Co-Investigator for the proposed project. Dr. Yang’s group will collect all of the BionanoSaphyr data proposed in the grant application. We estimate that this will entail approximately 1000-fold coverage of the human genome each year. For this, we will receive frozen cell pellets, prepare samples and run approximately 12 Saphyr flow cells per year. We will collect the data and run the Bionano data analysis pipeline to localize fluorescent incorporation signals on mapped DNA molecules. We will send this data to your lab for identification of replication tracks and analysis of origin and fork locations.