Bright and switchable fluorophores for highly multiplexed super-resolution microscopy towards molecular interaction imaging

This project aims to develop a palette of bright switchable fluorescent probes for parallel, multiplexed super-resolution imaging at the molecular level. We will concurrently improve the photon budget, decouple fluorescence switching from photochemistry and fine-tune the emission spectra of the dyes in the three specific aims to collectively provide high resolution and multiplexity. It will allow biomedical researchers, for the first time, to achieve simultaneous multi-color imaging of 6 molecular labels at 10-nm spatial resolution. System performance will be investigated by imaging nanoscale engineered samples, and multi-protein interactions in cells.