Cell Type Specific Genetic Manipulation to Dissect Cholinergic Interneuron Function and Plasticity in a Symptomatic Model of DYT1 Dystonia

Project: Research project

Project Details

Description

As outlined in your proposal, my laboratory will complete all experiments listed in Experiment Sets 2.3 and 2.4. These studies will define the intrinsic properties of surviving cholinergic interneurons, and the response of these cells to excitatory synaptic inputs. Included in this work, we will map the synaptic inputs to surviving cholinergic interneurons using mono-synaptic rabies virus methods. The ability to map a form of structural plasticity in a dystonia model is particularly exciting, as aberrant plasticity has long been hypothesized as a mechanism of dystonic physiology. All methods proposed are currently being pursued in my laboratory, so I do not anticipate any technical obstacles to the completion of the work.
Beyond the specific experiments proposed, we look forward to pursuing the logical future steps based on initial results, and to providing input on other experiments you are pursuing (e.g., DREADDs) that we also have experience pursuing. To facilitate these interactions and amplify the power of the multidisciplinary studies proposed, I will also participate in regular Skype conferences with other principals to share data and insights.
StatusActive
Effective start/end date2/1/211/31/26

Funding

  • University of Texas Southwestern Medical Center (GMO210305 PO 0000002283//1R01NS110853-01A1)
  • National Institute of Neurological Disorders and Stroke (GMO210305 PO 0000002283//1R01NS110853-01A1)

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