Center for 3D Structure and Physics of the Genome

The Marko lab is subcontracted to carry out theoretical modeling of the structure and mechanics of chromosomes and cell nuclei, and to carry out micromechanical experiments on isolated chromosomes, using unique facilities at Northwestern, and following the experimental approach described in the proposal grant. Micropipette-based micromanipulation and force measurement will be used to analyze the mechanics and structure of chromosomes isolated from the cells described in the proposal. The subcontract budget will support one graduate student at Northwestern who will be responsible for carrying out micromanipulation and imaging experiments, plus some analysis and modeling. The budget will also provide a small amount of academic year salary support for Prof. Marko and a small materials budget to cover the cost of materials for the experiments.