Project Details
Description
a. Objectives and research hypothesis
a1. Objectives
The RedEnuc project aims to decipher the physical mechanisms at play in enucleation during mammalian erythropoiesis: the process red blood cells (RBCs) are generated. Our general objective is to elucidate the role of the mechanical constraints exerted on the erythroblasts / nascent reticulocytes on the extrusion and detachment of nuclei. Our specific objectives are:
1. To develop an in vitro approach to quantitatively measure the physical forces experienced by erythroblasts and nascent reticulocytes,
2. To highlight the role of the forces experienced in a crowded environment and triggered mechanosensitive response in the cells on nuclear extrusion,
3. To elucidate how external squeezing and shearing forces contribute to nucleus detachment,
4. To develop a multiscale computational model to predict the acto-myosin generated internal active force that drives nucleus extrusion and its dependence on mechanosensing, and to predict the external squeezing and shearing forces that promote nucleus detachment.
Status | Active |
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Effective start/end date | 4/1/23 → 3/31/26 |
Funding
- National Science Foundation (PHY-2210369-002)
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